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Quantifying capital vs. income breeding: new promise with stable isotope measurements of individual amino acids

Citation

Whiteman, John (2020), Quantifying capital vs. income breeding: new promise with stable isotope measurements of individual amino acids, Dryad, Dataset, https://doi.org/10.5061/dryad.j3tx95xcb

Abstract

1. Capital breeders accumulate nutrients prior to egg development, then use these stores to support offspring. In contrast, income breeders rely on local nutrients consumed contemporaneously with offspring development. Understanding such nutrient allocations is critical to assessing wildlife reliance on different habitats.

2. Despite the contrast between these strategies, it remains challenging to trace nutrients from endogenous stores or exogenous food intake into offspring. Here, we tested a new solution to this problem.

3. Using tissue samples collected opportunistically from wild emperor penguins (Aptenodytes forsteri), which exemplify capital breeding, we hypothesized that the stable carbon (δ13C) and nitrogen (δ15N) isotope values of individual amino acids (AA) in endogenous stores (e.g., muscle) and in egg yolk and albumen reflect the nutrient sourcing that distinguishes capital versus income breeding. Unlike other methods, this approach does not require untested assumptions or diet sampling.

4. We found that over half of essential AA had δ13C values that did not differ between muscle and yolk or albumen, suggesting that most of these AA were directly routed from muscle into eggs. In contrast, almost all non-essential AA differed in δ13C values between muscle and yolk or between muscle and albumen, suggesting de novo synthesis. Over half of AA that have labile nitrogen atoms (i.e., “trophic” AA) had higher δ15N values in yolk and albumen than in muscle, suggesting that they were transaminated during their routing into egg tissue. This effect was smaller for AA with less labile nitrogen atoms (i.e., “source” AA).

5. Our results indicate that the δ15N offset between trophic-source AA (Δ15Ntrophic-source) may provide an index of the extent of capital breeding. The value of emperor penguin Δ15NPro-Phe was higher in yolk and albumen than in muscle, reflecting the mobilization of endogenous stores; in comparison, the value of Δ15NPro-Phe was similar across muscle and egg tissue in previously-published data for income-breeding herring gulls (Larus argentatus smithsonianus). Our results provide a quantitative basis for using AA δ13C and δ15N, and isotopic offsets among AA (e.g., Δ15NPro-Phe), to explore the allocation of endogenous versus exogenous nutrients across the capital versus income spectrum of avian reproduction.

Methods

These are stable carbon isotope and stable nitrogen isotope values measured in amino acids from hydrolyzed proteins in samples of emperor penguin egg yolk, egg albumen, and skeletal muscle.

Funding

Institut Polaire Français Paul Emile Victor, Award: 109

Natural Sciences and Engineering Research Council of Canada, Award: 2017-04430

Institut Universitaire de France

University of New Mexico