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Automated total and heterotrophic soil respiration in semi-arid shrubland and annual invasive patches

Citation

Mauritz, Marguerite; Lipson, David A (2021), Automated total and heterotrophic soil respiration in semi-arid shrubland and annual invasive patches, Dryad, Dataset, https://doi.org/10.5061/dryad.jdfn2z387

Abstract

Soil respiration (Rs) is the largest terrestrial source of carbon (C) flux to the atmosphere but our understanding of Rs controls with shifts in plant-community composition remains limited. We used high frequency soil respiration measurements and root exclusion to evaluate how Rs component fluxes, autotrophic respiration (Ra) and heterotrophic respiration (Rh), vary between a perennial semi-arid shrub community and annual invasive community. 

Methods

This study was conducted at the San Diego State University Santa Margarita Ecological Reserve in Riverside County, CA, USA (33°26’29.88” N, 117°9’51.38” W, Elevation: 393m). Disturbance is low at the reserve and the area has not burned in at least the last 50 years. The site where the study was conducted was flat with the central area was dominated by invasive annuals and native shrubs around the perimeter of the area. The climate is Mediterranean; summers are hot and dry, winters are cool and receive the majority of rainfall. In the two growing seasons that the study was conducted, total precipitation for the area was 380mm in 2009/2010 and 600mm in 2010/2011 with a mean temperature of 17°C in both years (www.ncdc.noaa.gov), compared to a regional long term average of 17°C and 420mm since 1895 (www.ncdc.noaa.gov). The soil is Las Posas rocky loam (NRCS Web Soil Survey), which tends to be moderately deep and well drained; weathered bedrock is at 50-60 cm depth (https://soilseries.sc.egov.usda.gov/OSD_Docs/L/LAS_POSAS.html). Coastal Sage Scrub vegetation is dominated by the native shrubs Artemisia californicaEriogonum fasciculatum and Salvia mellifera; intershrub spaces contain invasive annual grasses Bromus madritensis var. rubens and forbs Centaurea solstitialis and Hirschfeldia incana. 

The site where this study was conducted was flat with the central area was dominated by invasive annuals and native shrubs around the perimeter of the area. Invasive annuals established at the site following disturbance from installation of a water pipeline. Pre-invasion, the inter-shrub patches would have consisted of bare soils, biological soil crusts, native bunch grasses and spring-flowering native annual forbs. Edaphic factors were similar between Shrubs and Annual patches with no significant differences in extractable carbon pools, organic matter, texture, pH and cation concentrations 

Soil respiration was measured under the canopy of 4 replicate shrub and 4 inter-shrub patches, dominated by invasive Annuals. Shrubs were paired with an inter-shrub patch within 2m and replicate Shrub and Annual patches were 4-5m apart to avoid spatial auto-correlation and ensure random sampling of the ecosystem (Strahm 2005). In each Shrub and Annual patch total respiration (Rs) was partitioned into heterotrophic (Rh) and autotrophic (Ra) with paired root exclusion and non-exclusion treatments immediately adjacent to each other (total of 16 collars). Root exclusion cores consisted of 20cm diameter PVC pipe installed to 30cm depth. Total respiration was measured in 20cm diameter collars installed to 5cm depth to minimize disturbance of vegetation and root structure. All collars were installed following the first rainfall in December 2009 and soils allowed to acclimate before measurements were initiated on 9 February 2010. For each pair of intact and root-exclusion chambers Ra contributions could be estimated as the difference between Rs and Rh (Ra = Rs – Rh).

 

Soil respiration was measured continuously with an LI-8100 Automated Soil CO2 flux system (LI-COR) on cycle that re-measured a chamber every four hours. Every flux measurement lasted two minutes with 10 second logging interval. Soil moisture (percent volumetric water content (% VWC), EC-5, Decagon Devices Inc.) and soil temperature (°C, LI-8100 thermistors) at 0-5cm depth inside each collar, and chamber air temperature (°C, LI-8100 thermistors) were measured concurrent with respiration measurements. Soil moisture probes were calibrated using a linear regression with monthly gravimetric water samples (0-15cm) collected near each collar (relationship between gravimetric and volumetric samples, R2 = 0.472) (Czarnomski et al. 2005).

Soil respiration data was recorded for one-and-a-half growing seasons, February 2010-June 2010 and September 2010 – July 2011. In September 2010 measurements were resumed slightly before the first rainfall event. Measurements in both years terminated at the end of the growing season after invasive annuals senesced, midday temperatures approached 50°C, further rain was unlikely, and all respiration rates were close to 1μmol COm-2s-1.

 

Fluxes were estimated with exponential curves fit to each two-minute flux measurement using Licor 8100 FileViewer software (Version 2.0.0, LI-COR Inc. 2004-2006). Flux values with an exponential R2 fit less than 0.97 or a coefficient of variation (CV) > 2 were removed (approximately 15% of all measurements). Further quality control was conducted using Matlab (Version 7.4.0 R2007a, The MathWorks Inc. 1984-2007) and fluxes were removed if values in one chamber deviated substantially from correlation with all the other chambers (Savage et al. 2008). For most chambers less than 4% of the data was removed this way, but for one chamber up to 20% of the data was removed. 

Soil moisture, soil temperature and chamber temperature data were also quality controlled and erratic values removed. When soil moisture or temperature was missing due to prolonged sensor failure, data was filled using averages from the 3 other chambers in the same treatment. Gap-filled soil temperature and moisture data were used in statistical analyses.

Soil respiration measurements depend on accurate chamber temperature measurements. In chambers with failed thermistors flux rates were therefore corrected with a correction formula from Licor using the average chamber temperature from the three other chambers in the same vegetation and root exclusion treatment. In one chamber the thermistor never recorded accurate temperatures and all fluxes were corrected this way. Most other chambers only had episodic thermistor failure and approximately 16% of all flux measurements were corrected for bad chamber temperatures.

Daily average fluxes were calculated for Rs and Rh and expressed as µmol CO2 m-2s-1. Ra was calculated as an absolute flux and as a percent of Rs. In July 2011 some chambers developed leaks due to UV degradation of tubing connections, particularly in the Annual patches. Ra was only calculated when there was at least one Rs-Rh pair.

Usage Notes

Missing values are marked by NA and exist due to bad flux measurements, instrument failure, or power outage