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Wing: A suitable non-lethal tissue type for repeatable and rapid telomere length estimates in bats

Citation

Power, Megan L. et al. (2020), Wing: A suitable non-lethal tissue type for repeatable and rapid telomere length estimates in bats, Dryad, Dataset, https://doi.org/10.5061/dryad.jdfn2z38v

Abstract

Telomeres are used increasingly in ecology and evolution as biomarkers for ageing and environmental stress, and are typically measured from DNA extracted from non-lethally sampled blood. However, obtaining blood is not always possible in field conditions and only limited amounts can be taken from small mammals, such as bats, which moreover lack nucleated red blood cells and hence yield relatively low amounts of DNA. As telomere length can vary within species according to age and tissue, it is important to determine which tissues serve best as a representation of the organism as a whole. Here, we investigated whether wing tissue biopsies, a rapid and relatively non-invasive tissue collection method, could serve as a proxy for other tissues when measuring relative telomere length (rTL) in the Egyptian fruit bat (Rousettus aegyptiacus). Telomeres were measured from blood, brain, heart, kidney, liver lung, muscle and wing, and multiple wing biopsies were taken from the same individuals to determine intra-individual repeatability of rTL measured by using qPCR. Wing rTL correlated with rTL estimates from most tissues apart from blood. Blood rTL was not significantly correlated with rTL from any other tissue. Blood and muscle rTL were significantly longer compared with other tissues, while lung displayed the shortest rTLs. Individual repeatability of rTL measures from wing tissue was high (>76%). Here we show the relationships between tissue telomere dynamics for the first time in a bat, and our results provide support for the use of wing tissue for rTL measurements.

Methods

Please see Materials & Methods of this paper for further details.

Usage Notes

Individual_Tissues_DataDryad.csv

Individual tissue z-score normalised telomere data. 

Wing_Repeatability_DataDryad.csv

Wing repeatability z-score normalised telomere data.