Description of the Data and file structure

This dataset includes raw OCT data files of the animals and morphological structures presented in the manuscript published.

Files are provided in two formats:

1. in the original raw .OCT data files as generated using the ThorImageOCT version 5.1.2.0 software provided for users of the Thorlabs Ganymede OCT system.
2. in 32-bit .tiff file format to be opened in ImageJ. This file format is for people that do not have access to the ThorImageOCT software, and contains all the raw data information as the original files, but lacks the complete 3D view function and additional features of the ThorImageOCT software.

All files uploaded here represent the raw data of 2D and 3D scans of intact Cassiopea jellyfish, as well as cut-out regions of select tissue regions, used for this study (figures 1-5 and supplementary figure 3). All files are named with their respective figure number and panel (if applicable). A brief description of each figure is found below, but for more detail of the anatomy of Cassiopea please see the supplementary material provided by Proceedings B, as well as the main text for further details on each figure and methods applied:
Figure 1 | 3D scan of an entire juvenile Cassiopea sp. medusa, using a FOV of 7×7×2.8 mm (X-Y-Z), a resolution of 350×350×1024 pixels, corresponding to a voxel size of 20×20×2.74 µm. A pre-made, animated 3D representation of this figure can be found in the supplementary material provided by Proceedings B (Movie 1).
Figure 2 | Rhopalia of Cassiopea medusae of ~4 cm (a) and ~1 cm (c) in diameter. (b) 2D scan of the rhopalium observed in panel (a). Panel (c-e) is of a smaller medusae, where the rhopalia canal is observed forking out into two parts that surround the rhopalium.
Figure 3 | (a) 3D scan of an oral arm with clearly formed oral groove\, as well as oral vesicles and secondary mouths covered in frigid digitata. (b) closeup of vesicle with a developing cluster of cassiosomes.
Figure 4 | (a) OCT scan of bell tissue with part of the subumbrella epidermis and mesoglea removed to improve scan penetration depth and clarity in this area. (b) Overview of bell tissue with subumbrella layer removed. The remaining gastrodermis from the radial canals was still attached to the exumbrella mesoglea showing how these canals branch out over the entire bell. A dense carpet of white granules was found in the exumbrella mesoglea underneath radial canals.
Figure 5 | (a) 3D scan of a rhopalia canal with the subumbrella layer removed\, revealing the density of symbiont clusters in anastomosing tissue vs. white granules in this tissue area. Panel (b) is derived from panel (a) and represents a single tomographic section to illustrate the different densities of symbiont clusters and white granules. (c) closeup-scan of symbiont clusters. (d) closeup-scan of white granules. A pre-made, animated 3D representation of figure 5a can be found in the supplementary material provided by Proceedings B (Movie 2).
Fig. S3 | (a) Overview of an intact vesicular lappet submerged in pure glycerol. (b) Cross-sectional scan of the lappet.