Dataset for: Ultrarapid detection of SARS-CoV-2 RNA using a reverse transcription-free exponential amplification reaction, RTF-EXPAR
Data files
Jul 19, 2022 version files 554.24 KB
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Figure_2.txt
394 B
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Figure_3_EXPAR_10_Sigma_Time.txt
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Figure_3_EXPAR_Normalised_Data.txt
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Figure_3_EXPAR_Raw_Data.txt
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Figure_3_LAMP_10_Sigma_Time.txt
443 B
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Figure_3_LAMP_Raw_Data.txt
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Figure_3_PCR_10_Sigma_Time.txt
443 B
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Figure_3_PCR_Normalised_Data.txt
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Figure_3_PCR_Raw_Data.txt
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Figure_4_EXPAR_10_Sigma_Time.txt
492 B
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Figure_4_EXPAR_Normalised_Data.txt
33.06 KB
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Figure_4_EXPAR_Raw_Data.txt
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Figure_4_LAMP_10_Sigma_Time.txt
529 B
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Figure_4_LAMP_Raw_Data.txt
59.67 KB
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Figure_4_PCR_10_Sigma_Time.txt
467 B
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Figure_4_PCR_Raw_Data.txt
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Figure_5_10_Sigma_Time.txt
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Figure_5_Normalised_Data.txt
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Figure_5_Raw_Data.txt
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Figure_S1_10_Sigma_Time.txt
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Figure_S1_Normalised_Data.txt
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Figure_S1_Raw_Data.txt
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Figure_S2_10_Sigma_Time.txt
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Figure_S2_Normalised_Data.txt
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Figure_S2_Raw_Data.txt
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Figure_S3_10_Sigma_Time.txt
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Figure_S3_Normalised_Data.txt
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Figure_S3_Raw_Data.txt
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Figure_S4_10_Sigma_Time.txt
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Figure_S4_Normalised_Data.txt
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Figure_S4_Raw_Data.txt
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Figure_S5_10_Sigma_Time.txt
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Figure_S5_Normalised_Data.txt
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Figure_S5_Raw_Data.txt
10.62 KB
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Figure_S6_10_Sigma_Time.txt
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Figure_S6_Normalised_Data.txt
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README.txt
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Abstract
A dataset is reported for a rapid isothermal method for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for COVID-19. The procedure uses an unprecedented reverse transcription–free (RTF) approach for converting genomic RNA into DNA. This involves the formation of an RNA/DNA heteroduplex whose selective cleavage generates a short DNA trigger strand, which is then rapidly amplified using the exponential amplification reaction (EXPAR). Deploying the RNA-to-DNA conversion and amplification stages of the RTF-EXPAR assay in a single step results in the detection, via a fluorescence read-out, of single figure copy numbers per microliter of SARS-CoV-2 RNA in under 10 min. In direct three-way comparison studies the assay has been found to be faster than both PCR and loop-mediated isothermal amplification (LAMP), while being just as sensitive. The assay protocol involves the use of standard laboratory equipment and is readily adaptable for the detection of other RNA-based agents.
Fluorescence data was collected and exported as a plain text file for data processing (primary data files). Normalised data was determined using normalise function in GraphPad Prism 9 (normalised data files). To analyze the data, a program in C# was developed (cs format files). Each run time was calculated to be the point at which the fluorescence signal was greater than 10 SDs from the baseline signal (10-sigma time).
No additional information is required but corresponding authors (Dafforn and Tucker) can be contacted if neccesary.