Quantitative assessment of trace and macro element compositions of Cassava (Manihot esculenta) storage roots enriched with Β-Carotene as influenced by genotypes and growing locations
Citation
Alamu, Emmanuel; Maziya-Dixon, Busie; Dixon, Alfred Gilbert (2022), Quantitative assessment of trace and macro element compositions of Cassava (Manihot esculenta) storage roots enriched with Β-Carotene as influenced by genotypes and growing locations, Dryad, Dataset, https://doi.org/10.5061/dryad.k3j9kd59r
Abstract
Methods
There was a selection of three storage roots (large—900–2300 g, medium—500–899 g, and small—200–499 g) and washed thoroughly with potable water to remove dirt and adhered sand particles before air-dried on a clean concrete floor. The peeling of the storage roots was done manually using a stainless-steel knife, and the peeled roots were rinsed with deionized water to remove any contaminants. After peeling, the roots were cut longitudinally (from the proximal end to the distal end) into four equal parts. Two opposite sections from each root of each genotype were taken, combined, manually chopped into small pieces, and mixed thoroughly. A batch of samples from this lot was chosen for the mineral profiling analysis.
The processed samples were placed in a petri dish for three days and dried in an uncorroded conventional oven at 40 oC. After drying, the samples were packed in labelled, mineral-free paper envelopes. The trace and the macro element content were determined using Inductively Coupled Optical Emission Spectrometry (ICP-OES). About 0.30 g of the dried sample was weighed into 50 mL screw-cap polypropylene tubes, and added 2.0 mL HNO3 and 0.5 mL H2O2 to initiate the sample digestion. The samples were made to a final volume of 25 mL with 18 MW.cm water before injection. The sample flow rate was 2.0 mL/min, and the total analysis time per sample was approximately 2.5 min. Each piece was run in duplicate. Al concentrations of more than 5–10 mg/kg are frequently associated with contaminant Fe. Thus, the concentration of Al of less than 10 mg/kg was used to determine the quality of the data, and all samples had an Al level of less than 10 mg/kg. The trace elements identified in all the genotypes and varieties investigated were Fe, Mn, B, Cu, Mo, Co, Ni, Zn, and Al, respectively. Besides, the macro elements identified in all the genotypes and varieties investigated were Ca, Mg, Na, K, P, and S, respectively.
Usage notes
There is no additional information needed for the usage of this dataset. There were no missing values.
Funding
Bill and Melinda Gates Foundation, Award: OPP1019962