Data for: A novel and ubiquitous miRNA-involved regulatory module ensures precise phosphorylation of RNA polymerase II and proper transcription
Data files
Apr 02, 2024 version files 36.34 KB
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README.md
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S1_Data.xlsx
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Abstract
Proper transcription orchestrated by RNA polymerase II (RNPII) is crucial for cellular development, which relies on the phosphorylation state of RNPII’s carboxyl-terminal domain (CTD). Sporangia, developed from mycelia, are essential for the destructive oomycetes Phytophthora, remarkable transcriptional changes are observed during the morphological transition. However, how these changes are rapidly triggered and their relationship with the versatile RNPII-CTD phosphorylation remain enigmatic. Herein, we found that Phytophthora capsici had undergone an elevation of Ser5-phosphorylation in its uncanonical heptapeptide repeats of RNPII-CTD during sporangia development, which subsequently changed the chromosomal occupation of RNPII and primarily activated transcription of certain genes. A cyclin-dependent kinase, PcCDK7, was highly induced and phosphorylated RNPII-CTD during this morphological transition. Mechanistically, a novel DCL1-dependent microRNA, pcamiR1, was found to be a feedback modulator for the precise phosphorylation of RNPII-CTD by complexing with PcAGO1 and regulating the accumulation of PcCDK7. Moreover, this study revealed that the pcamiR1-CDK7-RNPII regulatory module is evolutionarily conserved and the impairment of the balance between pcamiR1 and PcCDK7 could efficiently reduce the growth and virulence of P. capsici. Collectively, this study uncovers a novel and evolutionarily conserved mechanism of transcription regulation that could facilitate correct development and identifies pcamiR1 as a promising target for disease control.
README: Data for: A novel and ubiquitous miRNA-involved regulatory module ensures precise phosphorylation of RNA polymerase II and proper transcription
Access this dataset on Dryad, DOI:10.5061/dryad.k3j9kd5gk
The dataset is about the study of a microRNA’s biological function in Phytophthora, which is derived from genetic study and phenotype analysis. The dataset containing, in separate sheets, the underlying numerical data and statistical analysis for Figure panels 1F, 2B, 2C, 2G, 3C, 3D, 3E, 3G, 4B, 4C, 4E, 4H, 5C, 5D, 5F, 5H, 6G, 8A, 8B, 8C, 8D, 9A, 9B and 9F in the manuscript titled “A novel and ubiquitous miRNA-involved regulatory module ensures precise phosphorylation of RNA polymerase II and proper transcription” (DOI: https://doi.org/10.1371/journal.ppat.1012138.
Description of the data and file structure
- Fig. 1F/2G/3G/5F/5H/6G/9F: The relative levels of different proteins in different samples. Units: 1.
- Fig. 2B/3C/3D/4B/4C/9A: The relative expression levels of different genes in different samples. Units: 1.
- Fig. 2C/3E/5C/8B/8D/9B: The sporangia number in different isolates. Units: per 0.24 mm^2.
- Fig. 4E: The GFP fluorescence signal under different treatments. Units: per unit.
- Fig. 4H: The ribosome occupancy distribution of different isolates, which is tested by the relative gene amount of each genomic fragment. Units: 1.
- Fig. 5D/8A: The colony diameter of different Phytophthra capsici isolates under different treatments. Units: mm.
- Fig. 8C: The lesion size caused by Phytophthora capsici under different treatments. Units: mm^2.
All the isolates or samples mentioned above are wild-type Phytophthora capsici isolates or different mutants. The “null” in the dataset means the data was missing in the single experimental repeat.
Sharing/Access information
Links to other publicly accessible locations of the data:
Code/Software
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