Data from: Establishment and maintenance of aphid endosymbionts after horizontal transfer is dependent on host genotype
Parker, Benjamin James, University of Oxford
McLean, Ailsa H.C., Emory University
Hrcek, Jan, University of Oxford
Gerardo, Nicole M., Emory University
Godfray, H. Charles J., University of Oxford
McLean, Ailsa H. C., University of Oxford
Published May 09, 2017 on Dryad.
Cite this dataset
Parker, Benjamin James et al. (2017). Data from: Establishment and maintenance of aphid endosymbionts after horizontal transfer is dependent on host genotype [Dataset]. Dryad. https://doi.org/10.5061/dryad.k50h1
Animal-associated microbial communities have important effects on host phenotypes. Individuals within and among species differ in the strains and species of microbes that they harbour, but how natural selection shapes the distribution and abundance of symbionts in natural populations is not well understood. Symbionts can be beneficial in certain environments but also impose costs on their hosts. Consequently, individuals that can or cannot associate with symbionts will be favoured under different ecological circumstances. As a result, we predict that individuals within a species vary in terms of how well they accept and maintain symbionts. In pea aphids, the frequency of endosymbionts varies among host-plant-associated populations (‘biotypes’). We show that aphid genotypes from different biotypes vary in how well they accept and maintain symbionts after horizontal transfer. We find that aphids from biotypes that frequently harbour symbionts are better able to associate with novel symbionts than those from biotypes that less frequently harbour symbionts. Intraspecific variation in the ability of hosts to interact with symbionts is an understudied factor explaining patterns of host–symbiont association.
The data file (in .csv format) contains 6 columns. The first column indicates which needle was used to inject symbionts (included as a random effect in the model). The second column indicates the genotype of the injected aphid (also included as a random effect). The third column shows which symbiont species (Regiella or Serratia) was injected into each aphid, and the fourth column gives the biotype of each aphid. The 5th column is the binary response variable that indicates whether an injected aphid's 10th offspring had a positive symbiont infection (as determined by PCR amplification). The 6th column is also a binary response variable indicating PCR amplification of the 10th offspring of a subset of aphids 3 generations after injection.