Based on molecular and morphological phylogenetic analyses a new genus of Theraphosidae is described, Pseudoclamoris gen. n. Tapinauchenius gigas and Tapinauchenius elenae are transferred to Pseudoclamoris and a new species of Pseudoclamoris from the Amazon Region is described: P. burgessi sp. n. Two new species of Tapinauchenius from the Caribbean are described: T. rasti sp. n. and T. polybotes sp. n. Tapinauchenius subcaeruleus is considered a nomen dubium. Psalmopoeinae subfamily is diagnosed based on molecular and morphological phylogenies, and Pseudoclamoris gen. n. and Ephebopus Simon, 1892 are included. A taxonomic key for Psalmopoeinae genera Tapinauchenius, Pseudoclamoris, Psalmopoeus, and Ephebopus is provided.