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Data from: Jasmonic acid regulation of the anti-herbivory mechanism conferred by fungal endophytes in grasses

Citation

Bastías, Daniel A. et al. (2019), Data from: Jasmonic acid regulation of the anti-herbivory mechanism conferred by fungal endophytes in grasses, Dryad, Dataset, https://doi.org/10.5061/dryad.k87m52h

Abstract

1. The most studied mechanism of protection against herbivores in grasses associated with Epichloë fungal endophytes has been the fungal production of alkaloids. However, the contribution of the plant immune responses on the level of resistance to herbivores in symbiotic grasses has been poorly explored. We studied the relationship between the plant hormone, jasmonic acid (JA), and Epichloë fungal endophytes on herbivore defenses in symbiotic grasses. We hypothesized that an exogenous application of methyl jasmonate (MeJA), an activator of the plant JA defense response, would increase the level of resistance of endophyte-symbiotic and non-symbiotic plants to a chewing insect. As Epichloë endophytes produce alkaloids, an enhancement of the JA defense would complement to the resistance given by these alkaloids. 2. Lolium multiflorum plants symbiotic and non-symbiotic with the endophyte Epichloë occultans were subjected to an exogenous application of MeJA followed by a challenge with the generalist chewing insect Spodoptera frugiperda. We measured the level of plant resistance to chewing insects, and the defenses conferred by host plants and fungal endophytes. 3. Symbiotic plants were more resistant to S. frugiperda than their non-symbiotic counterparts. However, despite the fact that the JA concentration significantly increased in all plants with the MeJA exposure, neither endophyte-symbiotic nor non-symbiotic plants showed an enhanced resistance to insects. Unexpectedly, the exposure of endophyte-symbiotic plants to MeJA led to a reduction in the concentration of loline alkaloids (i.e. N-formyllolines and N-acetylnorlolines), consequently decreasing the level of plant resistance to the herbivore. 4. Synthesis. Our results suggest that, rather than complementing the alkaloid-based defense, the JA hormone weakens the anti-herbivore mechanism conferred by endophytes. The present study highlights that the interaction between the JA hormone and the presence of leaf fungal endophytes can be of importance for the effectiveness of the anti-herbivore defenses of symbiotic plants.

Usage Notes

Location

Argentina
Buenos Aires