Microsatellite profile of Plasmopara viticola isolates collected in Italy
Cite this dataset
Toffolatti, Silvia Laura et al. (2022). Microsatellite profile of Plasmopara viticola isolates collected in Italy [Dataset]. Dryad. https://doi.org/10.5061/dryad.kh189328s
Downy mildew, caused by the oomycete Plasmopara viticola, is one of the most serious threat to viticulture. P. viticola is native to North America and causes the most severe damages to the Eurasian grapevine, Vitis vinifera. In this work, we investigated the genetic structure of the pathogen in Italy by collecting 96 P. viticola strains in twelve different regions. Based on microsatellite characterization (19 microsatellites), we confirmed that the Italian population of P. viticola is composed of two separate subpopulations and determined that these two cluster differ for geographic and climatic conditions. These populations could have been originated from the introduction of two separate populations or, more likely, from the evolution of the original population that reached Europe at the end of the XIX century. The pathogen showed a great evolutionary potential, as highlighted by the regular occurrence of sexual reproduction, panmictic structure within cluster and absence of clones. These results confirm that the P. viticola has a strong potential for adaptation and suggest the integration of all the tools (genetic, agronomic and chemical) for achieving a proper disease management.
In this file, you can find information on the origin of the 96 strains analysed and their microsatellite profiles (19 microsatellites). Detailed information on the isolate and their characterization are provided in the paper (https://doi.org/10.1111/aab.12567). Briefly, the 96 isolates analysed in this study were collected from 96 different vineyards located in twelve different Italian regions. The isolates, derived from single lesions on a randomly chosen leaf, were maintained in the culture collection of the Department of Agricultural and Environmental Sciences (DiSAA, University of Milan, Italy). Following DNA extraction, the isolates were genotyped at INRA Centre de Bordeaux-Aquitaine, UMR Santé et Agroécologie du Vignoble, France with 31 microsatellites but only the results of 19 of them (PV14, ISA, PV17, PV39, PV31, PV16, PV91, PV147, PV148, PV93, PV141, PV65, PV104, PV88, PV83, PV142, PV139, PV127, PV101) were retained due to missing data problems. The software GENEMAPPER 4.0 (Applied Biosystems) was used to analyse the chromatograms and record the allele sizes in bp.
The dataset consists of a csv format file that can be opened with excel, where the isolate N, geographic origin, grapevine variety, cluster and length of the 19 microsatellite markers are reported.
Università degli Studi di Milano