This version of the README file was generated on 07-22-24 by Jessamyn Perlmutter.

General Information

1. Title of Dataset: A single amino acid polymorphism in natural Metchnikowin alleles of Drosophila results in systemic immunity and life history tradeoffs
2. Author Information:

   1. Principal investigator contact information

      Name: Robert Unckless

      Institution: University of Kansas

      Address: Lawrence, KS, USA

      Email: unckless@ku.edu

   2. First author contact information

      Name: Jessamyn Perlmutter

      Institution: University of Kansas

      Address: Lawrence, KS, USA

      Email: jessie.perlmutter@gmail.com

3. Dates of data collection: 2020-2023
4. Location of data collection: University of Kansas, Lawrence
5. Funding sources that supported the collection of the data: NIH R01 AI139154, P30 GM110761, K-INBRE P20 GM103418, NSF DBI 2109772
6. General description of data from this data set (https://doi.org/10.5061/dryad.kkwh70sb5). This dataset contains the supplemental information associated with the manuscript publishes in PLOS Genetics (10.1371/journal.pgen.1011155), with an earlier preprint on bioRxiv (https://doi.org/10.1101/2023.01.16.524277). The contents include raw data underlying graphs, R code used to analyze results, and tables with statistical outputs of all analyses. All R code is accompanied by corresponding .csv files with the data.

Sharing/Access information

1. Licenses/restrictions placed on this data: CC0 1.0 Universal (CC0 1.0) Public Domain

2. Links to publications that cite or use the data:

   Perlmutter, J. I., Chapman, J. R., Wilkinson, M. C., Nevarez-Saenz, I., & Unckless, R. L. (2024). A single amino acid polymorphism in natural Metchnikowin alleles of Drosophila results in systemic immunity and life history tradeoffs. PLOS Genetics. (https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1011155)

3. Links to other publicly accessible locations of the data: None

4. Was data derived from another source? No

   A. If yes, list source(s): N/A

5. Recommended citation for this dataset:

Perlmutter, J. I., Chapman, J. R., Wilkinson, M. C., Nevarez-Saenz, I., & Unckless, R. L. (2024). Data from: A single amino acid polymorphism in natural Metchnikowin alleles of Drosophila results in systemic immunity and life history tradeoffs. Dryad Digital Repository. https://doi.org/10.5061/dryad.kkwh70sb5

Data & File Overview

1. File List:

A) Figures1BS2A-D.LifeHistory.R

B) Figures1CS3A_B.Longevity.R

C) Figures2A-F3A-D.MaleInfections.R

D) Figures4A_B.Male_FemaleInfections.R

E) Figures5A-C.MicrobialLoad.R

F) FiguresS4_S6_S10.SurvivalPlot.R

G) FigureS5.AdditionalInfections.R

H) FigureS7.FemaleInfections.R

I) FigureS8.FemaleSurvivalPlots.R

J) FigureS9.Male_FemaleSurvivalCurves.R

K) FigureS11B.GeneExpression.R

L) Figures1BS2A-D.LifeHistory.csv

M) Figures1CS3A.LongevityBlockA.csv

N) Figures1CS3B.LongevityBlockB.csv

O) MtkLongevityDates.BlockA.csv

P) MtkLongevityDates.BlockB.csv

Q) Figures2A-F3A-D.MaleInfections.csv

R) Figures4A_B.Male_FemaleInfections.csv

S) Figures5A-C.MicrobialLoad.csv

T) FigureS5.AdditionalInfections.csv

U) FigureS7.FemaleInfections.csv

V) FigureS11B.GeneExpression.csv

2. Relationship between files, if important:

A) Data underlying all figures is included in .csv files (Files L-V, above).

B) R code used to analyze all of the data .csv files is included (Files A-K, above).

C) List of corresponding data and analysis files:

1. Figures 1B and S2: files A and L
2. Figures 1C and S3: files B, M-P
3. Figures 2 & 3: files C & Q
4. Figure 4: files D & R
5. Figure 5: files E & S
6. Figures S4, S6, & S10: files F and C
7. Figure S5: files G & T
8. Figure S7: files H & U
9. Figure S8: files I & U
10. Figure S9: files J & R
11. Figure S11B: files K & V

3. Additional related data collected that was not included in the current data package: None

4. Are there multiple versions of the dataset? No

Data-Specific Information

Software and packages used: Data analysis and figure generation were performed in R v4.2.2, using several packages: ggplot2 (version 3.4.0), cowplot (version 1.1.1), car (version 3.1.1), SurvMiner (version 0.4.9), MultComp (version 1.4.20), ggExtra (version 0.10.0), ggtext (version 0.1.2), emmeans (version 1.8.3), stringr (version 1.5.0), and SurvMisc (version 0.5.6). Exact packages used for each figure may be found in the corresponding R file. Refer to PLOS Genetics publication for citations of these packages.

Information for Files A-K: Data analysis code in .R files

1. Description: All .R files used to analyze data from .csv files, including creation of figures and statistical analysis.
2. Variables, data codes, formats, & abbreviations:
   1. For data corresponding to each .R file, see “Data and File Overview” section, part 2.
   2. Variables are either created and defined in the code or details can be read below in the Information sections below for corresponding .csv data files.

Information for File L: Figures1BS2A-D.LifeHistory.csv

1. Description: Life history data underlying Figures 1B and S2, including sample information, life history data, and calculations of survival rates and adult sex ratios.
2. Variables, data codes, formats, & abbreviations:
   1. Strain: Unique strain code, with 3 strain codes for each genotype grouping: Unedit (P allele), Edit (R allele), deletion (null alleles)
   2. StrainType: genotype groupings of the individual strains: Unedit (P allele), Edit (R allele), deletion (null alleles)
   3. Vial: Biological replicate number within each strain and block. Each replicate follows all of the offspring of a single female from egg to death.
   4. Eggs: Number of eggs laid by a single female
   5. Hatched: Number of eggs hatched into larvae
   6. Block: Experimental replicates (A or B), with each replicate performed on different days
   7. hatchrate: Percent of eggs hatched into larvae (Hatched/Eggs*100)
   8. pupaeD8: Number of pupae formed in a vial
   9. L-P_Rate: Percent survival from larval to pupal stages (pupaeD8/Hatched*100)
   10. E-P_Rate: Percent survival from egg to pupal stages (pupae D8/Eggs*100)
   11. Adults: Total number of offspring reaching adulthood.
   12. M: Of the adults, the number that are male.
   13. F: Of the adults, the number that are female.
   14. P-A_Rate: Percent survival from pupal to adult stages (Adults/pupaeD8*100)
   15. L-A_Rate: Percent survival from larval to adult stages (Adults/Hatched*100)
   16. E-A_Rate: Percent survival from egg to adult stages (Adults/Eggs*100)
   17. NotHatched: Percent of eggs not hatched (100-hatchrate)
   18. NotPupated: Percent of hatched larvae that did not pupate ((Hatched-pupaeD8)/Eggs*100)
   19. NotEclosed: Percent of pupae that did not emerge as adults ((pupaeD8-Adults)/pupaeD8*100)
   20. PropAdult: Percent of eggs that reached adulthood (Adults/Eggs*100)
   21. sum: Percent of offspring that either survived to adulthood or died earlier, to check prior numbers (NotHatched+NotPupated+NotEclosed+PropAdult)
   22. SexRatio: Male to female sex ratio among adults (M/F)
   23. PropFem: Proportion of adults that are female (F/Adults)

Information for File M: Figures1CS3A.LongevityBlockA.csv

1. Description: Life history data underlying Block A of offspring adult longevity, from day of emergence from pupae to death. Corresponds to figures 1C and S3A.
2. Variables, data codes, formats, & abbreviations:
   1. Strain: Unique strain code, with 3 strain codes for each genotype grouping: Unedit (P allele), Edit (R allele), deletion (null alleles)
   2. StrainType: genotype groupings of the individual strains: Unedit (P allele), Edit (R allele), deletion (null alleles)
   3. Vial: Biological replicate number within each strain and block. Each replicate follows all of the offspring of a single female.
   4. Remaining Columns: The remaining columns are in pairs of matching numbers indicating number of days into adulthood. For example, “0” indicates the starting number of adults that had just emerged on day 0, and “2” indicates 2 days into adulthood. This goes until day 122, when all flies were finally confirmed as dead. Columns are paired, with two columns for each day. The left column of each pair is the number of living males. The right column of each pair is the number of living females. Numbers were recorded until the first day that all flies were dead from a given replicate. Remaining days are left blank for a given replicate once all flies are dead.
   5. Empty Data Cells: The data for these cells is empty as all flies were already recorded as dead. Code in the .R files for analyzing the data accounts for these empty cells, but could still work if the cells were filled with “0”.

Information for File N: Figures1CS3B.LongevityBlockB.csv

1. Description: Life history data underlying Block B of offspring adult longevity, from day of emergence from pupae to death. Corresponds to figures 1C and S3B.
2. Variables, data codes, formats, & abbreviations:
   1. Strain: Unique strain code, with 3 strain codes for each genotype grouping: Unedit (P allele), Edit (R allele), deletion (null alleles)
   2. StrainType: genotype groupings of the individual strains: Unedit (P allele), Edit (R allele), deletion (null alleles)
   3. Vial: Biological replicate number within each strain and block. Each replicate follows all of the offspring of a single female.
   4. Remaining Columns: The remaining columns are in pairs of matching numbers indicating number of days into adulthood. For example, “0” indicates the starting number of adults that had just emerged on day 0, and “7” indicates 7 days into adulthood. This goes until day 82, when all flies were finally confirmed as dead. Columns are paired, with two columns for each day. The left column of each pair is the number of living males. The right column of each pair is the number of living females. Numbers were recorded until the first day that all flies were dead from a given replicate. Remaining days were filled in with “0”.

Information for File O: MtkLongevityDates.BlockA.csv

1. Description: File with “Remaining Columns” From File P copied over into additional file for ease of coding. Corresponds to Figures 1C and S3A, and Block A of the experiment.
2. Variables, data codes, formats, & abbreviations:
   1. See “Remaining Columns” section for “Information for File M” for further information

Information for File P: MtkLongevityDates.BlockB.csv

1. Description: File with “Remaining Columns” From File P copied over into additional file for ease of coding. Corresponds to Figures 1C and S3B, and Block B of the experiment.
2. Variables, data codes, formats, & abbreviations:
   1. See “Remaining Columns” section for “Information for File Q” for further information

Information for File Q: Figures2A-F3A-D.MaleInfections.csv

1. Description: Data for infections of male flies underlying Figures 2 & 3. Data indicates the number of surviving males in each replicate from a starting number of 10 over time. Leftmost columns indicate important data for each sample.
2. Variables, data codes, formats, & abbreviations:
   1. Vial: Replicate number, out of total on spreadsheet, indicating a single unique biological replicate out of all data.
   2. Gt: Genotype, grouped by general descriptor of Mtk or other relevant genotype
      1. 1 bp del: Mtk null allele strain with single base pair deletion
      2. 6 bp del: Mtk null allele strain with 6 base pair deletion
      3. 17 bp del: Mtk null allele strain with 17 bp deletion
      4. R1/R2/R3: Independently-derived CRISPR Mtk arginine (R, or edit) allele strains, 3 independent strains total (R1/R2/R3)
      5. P1/P2/P3: Independently-derived CRISPR Mtk proline (P, or unedit) allele strains, 3 independent strains total (P1/P2/P3)
      6. Spz: Strain with null allele for spatzle gene
      7. MyD88: Strain with null allele for myd88 gene
   3. Line: Unique number identifiers corresponding to each independent Mtk fly strain tested, or Spz or MyD88 strains.
   4. Treatment: Infection or control treatment used for a given sample
      1. JU2- not used in this study, indicates a likely Penicillium fungal strain isolated from a fly.
      2. B. bassiana= Beauveria bassiana filamentous fungus
      3. 20% Glycerol= 20% Glycerol negative infection control for filmentous fungi
      4. F. oxysporum= Fusarium oxysporum filamentous fungus
      5. A. fumigatus= Apergillus fumigatus filamentous fungus
      6. A. flavus= Aspergillus flavus filamentous fungus
      7. G. pseudocandidus= Galactomyces pseudocandidus yeast
      8. C. glabrata= Candida glabrata yeast
      9. PDB= Potato Dextrose Broth negative control for yeast
      10. B. thuringiensis= Bacillus thuringiensis bacteria
      11. P. rettgeri= Providencia rettgeri bacteria
      12. LB= Luria Broth negative control for bacteria
      13. E. faecalis= Enterococcus faecalis bacteria
      14. S. marsescens= Serratia marcescens bacteria
      15. E. faecalis dnaK= Enterococcus faecalis bacteria with null allele in dnak gene, k12 strain background
      16. E. faecalis k12= Enterococcus faecalis bacteria of k12 WT strain
   5. Replicate: Replicate number within each sample set of a given Gt x Treatment x Day combination
   6. DayCollected: Date flies were collected and set aside for experiment
   7. DayInfected: Date flies were initially infected (Day 0)
   8. OD600: Optical density at 600 nm for a given culture (if bacteria or yeast) or conidia/mL for filamentous fungi.
      1. Cells left blank for negative controls, which are at a baseline OD600 of 0
   9. Day# Columns: Surviving flies on Day# post-infection. D0 indicates day 0, the day of infection. All vials start with 10 flies at D0.
      1. Cells left blank indicate the end of a given experiment. Some experimental survival rates were recorded for longer than others, depending on the pathogen species and experimental replicate. Blank cells indicate days survival was no longer recorded.

Information for File R: Figures4A_B.Male_FemaleInfections.csv

1. Description: Infection survival data corresponding to experiments done in Figures 4 and S9.
2. Variables, data codes, formats, & abbreviations:
   1. Vial: Replicate number, out of total on spreadsheet, indicating a single unique biological replicate out of all data.
   2. Gt: Genotype, grouped by general descriptor of Mtk or other relevant genotype
      1. 1 bp del: Mtk null allele strain with single base pair deletion
      2. 6 bp del: Mtk null allele strain with 6 base pair deletion
      3. 17 bp del: Mtk null allele strain with 17 bp deletion
      4. R1/R2/R3: Independently-derived CRISPR Mtk arginine (R, or edit) allele strains, 3 independent strains total (R1/R2/R3)
      5. P1/P2/P3: Independently-derived CRISPR Mtk proline (P, or unedit) allele strains, 3 independent strains total (P1/P2/P3)
      6. Spz: Strain with null allele for spatzle gene
      7. MyD88: Strain with null allele for myd88 gene
   3. MtkAllele: Broad grouping of similar allele types
      1. Null= deletion or Mtk null strains
      2. Edit= arginine (R) Mtk strains
      3. Unedit= proline (P) Mtk strains
      4. Spz= spatzle gene null strain
      5. MyD88= myd88 gene null strain
   4. Line: Unique number identifiers corresponding to each independent Mtk fly strain tested, or Spz or MyD88 strains.
   5. Treatment: Infection or control treatment used for a given sample
      1. B. bassiana= Beauveria bassiana filamentous fungus
      2. A. fumigatus= Apergillus fumigatus filamentous fungus
      3. 20% Glycerol= 20% Glycerol negative infection control for filmentous fungi
   6. Replicate: Replicate number within each sample set of a given Gt x Treatment x Day x Sex combination
   7. Sex: Sex of flies from the given sample (F for females, M for males)
   8. DayCollected: Date flies were collected and set aside for experiment
   9. DayInfected: Date flies were initially infected (Day 0)
   10. Day# Columns: Surviving flies on Day# post-infection. D0 indicates day 0, the day of infection. All vials start with 10 flies at D0.

Information for File S: Figures5A-C.MicrobialLoad.csv

1. Description: Data from microbial load experiment corresponding to Figure 5.
2. Variables, data codes, formats, & abbreviations:
   1. Plate#: Indicates an individual sample across all replicate experiments.
   2. Block: Indicates an experimental replicate, with the entire experiment replicated 3 times (Blocks A, B, C).
   3. Replicate: Individual samples of a given FlyGenotype x Microbe x DateInfected combination (3 replicates for each grouping: a, b, or c).
   4. FlyGenotype:
      1. 1 bp del: Mtk null allele strain with single base pair deletion
      2. 6 bp del: Mtk null allele strain with 6 base pair deletion
      3. 17 bp del: Mtk null allele strain with 17 bp deletion
      4. R1/R2/R3: Independently-derived CRISPR Mtk arginine (R, or edit) allele strains, 3 independent strains total (R1/R2/R3)
      5. P1/P2/P3: Independently-derived CRISPR Mtk proline (P, or unedit) allele strains, 3 independent strains total (P1/P2/P3)
      6. Spz: Strain with null allele for spatzle gene
      7. MyD88: Strain with null allele for myd88 gene
   5. MtkAllele:
      1. Null= deletion or Mtk null strains
      2. Edit= arginine (R) Mtk strains
      3. Unedit= proline (P) Mtk strains
      4. MyD88= myd88 gene null strain
   6. FlyLine: Unique number identifiers corresponding to each independent Mtk fly strain tested or MyD88 strain.
   7. Sex: M for males (all male in this experiment)
   8. NumberFlies: Number of individual flies pooled for each sample (3 for all samples)
   9. Microbe: Pathogen species used to infect the flies
      1. C. glabrata= Candida glabrata yeast
      2. P. rettgeri= Providencia rettgeri bacteria
      3. E. faecalis= Enterococcus faecalis bacteria
   10. MicrobeStrain: Strain number of each microbe, uniquely used within the Unckless lab.
   11. Media: Media used to grow the indicated pathogen and the vehicle used for infection
       1. PDB= Potato Dextrose Broth
       2. LB= Luria Broth
   12. Temp: Temperature used to grow pathogens
       1. 30: 30 degrees Celsius
       2. 37: 37 degrees Celsius
   13. DateInfected: Date the flies were infected.
   14. HomogenateVol: Volume of liquid PBS used to homogenize the pooled flies in each sample, in microliters
   15. DilFactor: Dilution factor of a homogenized sample, if the sample was diluted in PBS before plating
   16. Colonies: Total number of microbial colonies or CFU’s from each sample.
       1. Only filled if counted by hand. Blank cells indicate a colony counter was used (done for later replicates)
   17. CFU.fly: Total number of microbial colonies or CFU’s, calculated per fly
       1. ”-“ symbol if no colonies grew
       2. Blank cells for later replicates, as this calculation was no longer used.
   18. CFU.mL: Total number of microbial colonies or CFU’s, calculated per mL of homogenate
       1. All samples from all replicates have this calculation, if microbes grew. This was the calculation used for the figure. Blank cells indicate no microbes grew from the sample.
   19. CFU.mL (direct readout): Total number of microbial colonies or CFU’s, calculated per mL of homogenate, as estimated by a robotic colony counter
       1. Blank cells in the first replicate before the counter was used, and blank in later replicates if no microbes grew in the sample.
   20. Final column: Replicate number for each given Microbe x DateInfected combination

Information for File T: FigureS5.AdditionalInfections.csv

1. Description: Infection survival data for male flies infected with additional microbial pathogens, corresponding to Figure S5.
2. Variables, data codes, formats, & abbreviations:
   1. Vial: Replicate number, out of total on spreadsheet, indicating a single unique biological replicate out of all data.
   2. Gt: Genotype, grouped by general descriptor of Mtk or other relevant genotype
      1. 1 bp del: Mtk null allele strain with single base pair deletion
      2. 6 bp del: Mtk null allele strain with 6 base pair deletion
      3. 17 bp del: Mtk null allele strain with 17 bp deletion
      4. R1/R2/R3: Independently-derived CRISPR Mtk arginine (R, or edit) allele strains, 3 independent strains total (R1/R2/R3)
      5. P1/P2/P3: Independently-derived CRISPR Mtk proline (P, or unedit) allele strains, 3 independent strains total (P1/P2/P3)
      6. Spz: Strain with null allele for spatzle gene
      7. MyD88: Strain with null allele for myd88 gene
   3. MtkAllele: Broad grouping of similar allele types
      1. Null= deletion or Mtk null strains
      2. Edit= arginine (R) Mtk strains
      3. Unedit= proline (P) Mtk strains
      4. Spz= spatzle gene null strain
      5. MyD88= myd88 gene null strain
   4. Line: Unique number identifiers corresponding to each independent Mtk fly strain tested, or Spz or MyD88 strains.
   5. Treatment: Infection or control treatment used for a given sample
      1. Note: The spreadsheet contains data from infections that were not ultimately included in the publication. Abbreviations explained here are those that pertain to the published data.
      2. Negative= Negative control
         1. LB (Luria Broth) for bacteria corresponding to the “Negative control” (performed on same day)
         2. YPD (Yeast extract, peptone, dextrose media) for yeast corresponding to the “Negative control” (performed on same day)
      3. Ly. fusiformis= Lysinibacillus fusiformis bacteria
      4. S. succinus= Staphylococcus succinus bacteria
      5. S. sciuri= Staphylococcus sciuri bacteria
      6. C. albicans= Candida albicans yeast
      7. L. brevis= Lactococcus brevis bacteria
      8. L. plantarum= Lactococcus plantarum bacteria
      9. YPD= Yeast extract, peptone, dextrose media, negative control for C. albicans
      10. LB= Luria broth media, negative control for most bacteria
      11. MRS= deMan-Rogosa-Sharpe media, negative control for Lactococcus bacteria
   6. Replicate: Replicate number within each sample set of a given Gt x Treatment x Day combination
   7. DayCollected: Date flies were collected and set aside for experiment
   8. DayInfected: Date flies were initially infected (Day 0)
   9. OD600: Optical density at 600 nm for a given culture (if bacteria or yeast) or conidia/mL for filamentous fungi.
      1. Cells filled with “0” for negative controls, which are at a baseline OD600 of 0.
   10. Day# Columns: Surviving flies on Day# post-infection. D0 indicates day 0, the day of infection. All vials start with 10 flies at D0.
       1. Cells left blank indicate the end of a given experiment. Some experimental survival rates were recorded for longer than others, depending on the pathogen species and experimental replicate. Blank cells indicate days survival was no longer recorded.

Information for File U: FigureS7.FemaleInfections.csv

1. Description: Data for female infection survival, corresponding to Figures S7 and S8.
2. Variables, data codes, formats, & abbreviations:
   1. Vial: Replicate number, out of total on spreadsheet, indicating a single unique biological replicate out of all data.
   2. Gt: Genotype, grouped by general descriptor of Mtk or other relevant genotype
      1. 1 bp del: Mtk null allele strain with single base pair deletion
      2. 6 bp del: Mtk null allele strain with 6 base pair deletion
      3. 17 bp del: Mtk null allele strain with 17 bp deletion
      4. R1/R2/R3: Independently-derived CRISPR Mtk arginine (R, or edit) allele strains, 3 independent strains total (R1/R2/R3)
      5. P1/P2/P3: Independently-derived CRISPR Mtk proline (P, or unedit) allele strains, 3 independent strains total (P1/P2/P3)
      6. Spz: Strain with null allele for spatzle gene
      7. MyD88: Strain with null allele for myd88 gene
   3. MtkAllele: Broad grouping of similar allele types
      1. Null= deletion or Mtk null strains
      2. Edit= arginine (R) Mtk strains
      3. Unedit= proline (P) Mtk strains
      4. Spz= spatzle gene null strain
      5. MyD88= myd88 gene null strain
   4. Line: Unique number identifiers corresponding to each independent Mtk fly strain tested, or Spz or MyD88 strains.
   5. Treatment: Infection or control treatment used for a given sample
      1. P. rettgeri= Providencia rettgeri bacteria
      2. E. faecalis= Enterococcus faecalis bacteria
      3. LB= Luria Broth, negative control for bacteria
      4. C. glabrata= Candida glabrata yeast
      5. PDB= Potato dextrose broth, negative control for yeast
      6. B. bassiana= Beauveria bassiana filamentous fungus
      7. A. fumigatus= Aspergillus fumigatus filamentous fungus
      8. 20% Glycerol= 20% glycerol negative control for filamentous fungi
   6. Replicate: Replicate number within each sample set of a given Gt x Treatment x Day combination
   7. DayCollected: Date flies were collected and set aside for experiment
   8. DayInfected: Date flies were initially infected (Day 0)
   9. OD600: Optical density at 600 nm for a given culture (if bacteria or yeast) or conidia/mL for filamentous fungi.
      1. Cells left blank for negative controls, which are at a baseline OD600 of 0
   10. Day# Columns: Surviving flies on Day# post-infection. D0 indicates day 0, the day of infection. All vials start with 10 flies at D0.
       1. Cells left blank indicate the end of a given experiment. Some experimental survival rates were recorded for longer than others, depending on the pathogen species and experimental replicate. Blank cells indicate days survival was no longer recorded.

Information for File V: FigureS11B.GeneExpression.csv

1. Description: Mtk gene expression data for experiment underlying Figure S11B.
2. Variables, data codes, formats, & abbreviations:
   1. Sample#: Number for each unique sample measured
   2. Allele: Mtk allele measured for all samples
   3. Strain: Unique number identifiers corresponding to each independent Mtk fly strain tested
   4. StrainType: Broad grouping of similar allele types
      1. Deletion= Mtk null strains
      2. Edit= arginine (R) Mtk strains
      3. Unedit= proline (P) Mtk strains
   5. Sex: Sex of flies from a given sample (F for female, M for male)
   6. Treatment: Infection or control treatment used for a given sample
      1. B. bassiana= Beauveria bassiana filamentous fungus
      2. Control= negative control (not infected)
      3. Deltact= 2^-(deltaCt) calculation for each of the samples from qPCR