Raw data from: Experimental evolution can enhance benefits of rhizobia to novel legume hosts
Quides, Kenjiro et al. (2021), Raw data from: Experimental evolution can enhance benefits of rhizobia to novel legume hosts, Dryad, Dataset, https://doi.org/10.5061/dryad.ksn02v73h
Legumes preferentially associate with and reward beneficial rhizobia in root nodules, but the processes by which rhizobia evolve to provide benefits to novel hosts remain poorly understood. Using cycles of in planta and in vitro evolution, we experimentally simulated lifestyles where rhizobia repeatedly interact with novel plant genotypes with which they initially provide negligible benefits. Using a fullfactorial replicated design, we independently evolved two rhizobia strains in associations with each of two Lotus japonicus genotypes that vary in regulation of nodule formation. We evaluated phenotypic evolution of rhizobia by quantifying fitness, growth effects, and histological features on hosts, and molecular evolution via genome resequencing. Rhizobia evolved enhanced host benefits and caused changes in nodule development in one of the four host-symbiont combinations, that appeared to be driven by reduced costs during symbiosis, rather than increased nitrogen fixation. Descendent populations included genetic changes that could alter rhizobial infection or proliferation in host tissues, but lack of evidence for fixation of these mutations weakens the results. Evolution of enhanced rhizobial benefits occurred only in a subset of experiments, suggesting a role for host-symbiont genotype interactions in mediating the evolution of enhanced benefits from symbionts.
Shoot biomass and number of nodules formed during passaging
Summary of data collected from both replicate passage lineages for each host and symbiont combination
in vitro growth rate measured for ancestral and derived symbionts.
Raw data from the greenhouse experiment comparing ancestral and derived phenotypes
Nodule culturing data from the greenhouse experiment
Carbon and Nitrogen isotope data from leaves of plants in the greenhouse experiment
Raw nodule histology data from the greenhouse experiment
University of California, Riverside, Award: EEOB Department Loomer and Newell Awards
Chapman University, Award: Grand Challenges Initiative
National Science Foundation, Award: DEB 1738009