Data from: Estimating bloodstain age in the short term based on DNA fragment length using nanopore sequencer
Data files
Apr 02, 2024 version files 10.89 GB
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10days.zip
485.07 MB
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14days.zip
463.24 MB
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1day.zip
794.67 MB
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21days.zip
320.24 MB
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28days.zip
620.04 MB
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2days.zip
404.58 MB
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2months.zip
738.75 MB
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3days.zip
327.56 MB
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3months.zip
351.29 MB
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4months.zip
355.13 MB
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5days.zip
232.42 MB
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6months.zip
743.56 MB
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7days.zip
405.56 MB
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alignment_summary.zip
135.61 MB
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README.md
8 KB
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w_1day.zip
1.40 GB
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w_2days.zip
974.34 MB
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w_3days.zip
717.73 MB
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w_5days.zip
555.03 MB
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w_8days.zip
861.37 MB
Abstract
We used a nanopore sequencer to quantify DNA fragments > 10,000 bp in size and then evaluated their relationship with short-term bloodstain age. Moreover, DNA degradation was investigated after bloodstains were wetted once with water. Bloodstain samples on cotton gauze were stored at room temperature and low humidity for up to 6 months. Bloodstains stored for 1 day were wetted with nuclease-free water, allowed to dry, and stored at room temperature and low humidity for up to 1 week. The proportion of fragments > 20,000 bp in dry bloodstains tended to decrease over time, particularly for fragments > 50,000 bp in size. This trend was modeled using a power approximation curve, with the highest R2 value (0.6475) noted for fragments > 50,000 bp in size; lower values were recorded for shorter fragments. The proportion of longer fragments was significantly reduced in bloodstains that were dried after being wetted once, and there was significant difference in fragments > 50,000 bp between dry conditions and once-wetted. This result suggests that even temporary exposure to water causes significant DNA fragmentation, but not extensive degradation. Thus, bloodstains that appear fresh but have a low proportion of long DNA fragments may have been wetted previously. Our results indicate that evaluating the proportion of long DNA fragments yields information on both bloodstain age and the environment in which they were stored.
README
These fastq files were used for analysis in this study.
A file with a "*" after it indicates that the files contain DNA CS (DNA control strand; 3.6 kb standard amplicon mapping the 3' end of the Lambda genome) sequences for quality check. The read count percentage calculations were performed after excluding these sequences.
A folder with a "w" prefix indicates that the fastq data are from the experiments (figure 2) in which the bloodstain was wetted with water.
"Alignment summary" folder includes the results of mapping with hg38 by the MinKNOW software.
Folder PATH listing
+---1day
| AJM454_pass_bc2cd138_0.fastq(*)
| ALB645_pass_20c53053_0.fastq(*)
| ALL990_pass_1a2f28eb_0.fastq(*)
| ANN476_pass_ed34f4ba_46227b6b_0.fastq(*)
+---2days
| ALB394_pass_929d1638_0.fastq(*)
| ALR682_pass_50fad96d_0.fastq(*)
| ANN386_pass_6e9ecc23_89da19c2_0.fastq(*)
| ANR037_pass_7afa0862_06d9d000_0.fastq(*)
+---3days
| AJM407_pass_ab643349_0.fastq(*)
| ALB405_pass_5c9ecc26_0.fastq(*)
| ANU671_pass_9e0f6da0_2ed6595f_0.fastq(*)
| ANX060_pass_0a211314_54dba230_0.fastq(*)
+---5days
| ALC054_pass_aa864ef9_0.fastq(*)
| ALL960_pass_d4836cab_0.fastq(*)
| AMX219_pass_03cca76e_c5c9e514_0.fastq
| ANK566_pass_ca4d85a9_79ee0342_0.fastq(*)
+---7days
| AJM455_pass_b8e0c36f_0.fastq(*)
| ALB710_pass_bc12d776_0.fastq(*)
| ALR175_pass_3dbed4ef_0.fastq(*)
| ANK215_pass_09b18fef_479fc77d_0.fastq(*)
+---10days
| ALC013_pass_557321c9_0.fastq(*)
| ANM449_pass_ec7b8135_0169951c_0.fastq(*)
| AOL145_pass_5ce9a6fb_ea26ef4a_0.fastq
| AOO261_pass_7a496160_90dccef5_0.fastq
+---14days
| AJO736_pass_8dda8496_0.fastq(*)
| ALB608_pass_9cd1060c_0.fastq(*)
| ALT654_pass_7ec3ce48_0.fastq(*)
| ANN273_pass_4cbce703_117b6910_0.fastq(*)
+---21days
| ALC072_pass_4f8beeab_0.fastq(*)
| ANT028_pass_7dbff7e2_d895ac13_0.fastq(*)
| ANU496_pass_7a62ef46_77febc71_0.fastq(*)
| AOL214_pass_de7e944e_b75bcd03_0.fastq
+---28days
| AJU547_pass_25edcf12_0.fastq(*)
| ALB270_pass_5a306b17_0.fastq(*)
| ALR652_pass_48dffbe9_0.fastq(*)
| AON611_pass_eb1217db_2344207a_0.fastq
+---2months
| ALM138_pass_22a42b73_0.fastq(*)
| AOL206_pass_922ab2db_04f992cf_0.fastq
| AOL534_pass_ccc161da_3d5166e1_0.fastq
| AOM261_pass_559ad00e_0ba2bf5b_0.fastq
+---3months
| ANG280_pass_1faa73da_0.fastq(*)
| ANO532_pass_6dfc9979_0.fastq(*)
| ANO756_pass_b7acb270_0.fastq(*)
| AOL584_pass_0540ecf7_ada5001a_0.fastq
+---4months
| ANG698_pass_253d9022_0.fastq(*)
| ANP242_pass_343f75a8_0.fastq(*)
| ANQ483_pass_48ae7bfa_0.fastq(*)
| AOT468_pass_8e792725_1286f3c4_0.fastq
+---6months
| ANG702_pass_54ea346b_0.fastq(*)
| ANH875_pass_0934c646_0.fastq(*)
| ANP446_pass_90fea4ca_0.fastq(*)
| AOV830_pass_91b38c3f_78d37fb2_0.fastq
+---w_1day
| FAW77777_pass_barcode08_8ca636cc_e83af5d2_0.fastq
| FAW81892_pass_barcode01_f810f4db_dc345a2f_0.fastq
| FAW81892_pass_barcode09_f810f4db_dc345a2f_0.fastq
| FAW81892_pass_barcode14_f810f4db_dc345a2f_0.fastq
+---w_2days
| FAW77777_pass_barcode09_8ca636cc_e83af5d2_0.fastq
| FAW81892_pass_barcode02_f810f4db_dc345a2f_0.fastq
| FAW81892_pass_barcode10_f810f4db_dc345a2f_0.fastq
| FAW81892_pass_barcode15_f810f4db_dc345a2f_0..fastq
+---w_3days
| FAW77777_pass_barcode10_8ca636cc_e83af5d2_0.fastq
| FAW81892_pass_barcode03_f810f4db_dc345a2f_0.fastq
| FAW81892_pass_barcode11_f810f4db_dc345a2f_0.fastq
| FAW81892_pass_barcode16_f810f4db_dc345a2f_0.fastq
+---w_5days
| FAW77777_pass_barcode11_8ca636cc_e83af5d2_0.fastq
| FAW81892_pass_barcode04_f810f4db_dc345a2f_0.fastq
| FAW81892_pass_barcode12_f810f4db_dc345a2f_0.fastq
| FAW81892_pass_barcode17_f810f4db_dc345a2f_0.fastq
+---w_8days
| FAW77777_pass_barcode12_8ca636cc_e83af5d2_0.fastq
| FAW81892_pass_barcode07_f810f4db_dc345a2f_0.fastq
| FAW81892_pass_barcode13_f810f4db_dc345a2f_0.fastq
| FAW81892_pass_barcode18_f810f4db_dc345a2f_0.fastq
+---alignment summary
+---1 day
| AJM454_pass_bc2cd138_0.txt
| ALB645_pass_20c53053_0.txt
| ALL990_pass_1a2f28eb_0.txt
| ANN476_pass_ed34f4ba_46227b6b_0.txt
+---2 days
| ALB394_pass_929d1638_0.txt
| ALR682_pass_50fad96d_0.txt
| ANN386_pass_6e9ecc23_89da19c2_0.txt
| ANR037_pass_7afa0862_06d9d000_0.txt
+---3 days
| AJM407_pass_ab643349_0.txt
| ALB405_pass_5c9ecc26_0.txt
| ANU671_pass_9e0f6da0_2ed6595f_0.txt
| ANX060_pass_0a211314_54dba230_0.txt
+---5 days
| ALC054_pass_aa864ef9_0.txt
| ALL960_pass_d4836cab_0.txt
| AMX219_pass_03cca76e_c5c9e514_0.txt
| ANK566_pass_ca4d85a9_79ee0342_0.txt
+---7 days
| AJM455_pass_b8e0c36f_0.txt
| ALB710_pass_bc12d776_0.txt
| ALR175_pass_3dbed4ef_0.txt
| ANK215_pass_09b18fef_479fc77d_0.txt
+---10 days
| ALC013_pass_557321c9_0.txt
| ANM449_pass_ec7b8135_0169951c_0.txt
| AOL145_pass_5ce9a6fb_ea26ef4a_0.txt
| AOO261_pass_7a496160_90dccef5_0.txt
+---14 days
| AJO736_pass_8dda8496_0.txt
| ALB608_pass_9cd1060c_0.txt
| ALT654_pass_7ec3ce48_0.txt
| ANN273_pass_4cbce703_117b6910_0.txt
+---21 days
| ALC072_pass_4f8beeab_0.txt
| ANT028_pass_7dbff7e2_d895ac13_0.txt
| ANU496_pass_7a62ef46_77febc71_0.txt
| AOL214_pass_de7e944e_b75bcd03_0.txt
+---28 days
| AJU547_pass_25edcf12_0.txt
| ALB270_pass_5a306b17_0.txt
| ALR652_pass_48dffbe9_0.txt
| AON611_pass_eb1217db_2344207a_0.txt
+---2 months
| ALM138_pass_22a42b73_0.txt
| AOL206_pass_922ab2db_04f992cf_0.txt
| AOL534_pass_ccc161da_3d5166e1_0.txt
| AOM261_pass_559ad00e_0ba2bf5b_0.txt
+---3 months
| ANG280_pass_1faa73da_0.txt
| ANO532_pass_6dfc9979_0.txt
| ANO756_pass_b7acb270_0.txt
| AOL584_pass_0540ecf7_ada5001a_0.txt
+---4 months
| ANG698_pass_253d9022_0.txt
| ANP242_pass_343f75a8_0.txt
| ANQ483_pass_48ae7bfa_0.txt
| AOT468_pass_8e792725_1286f3c4_0.txt
+---6 months
| ANG702_pass_54ea346b_0.txt
| ANH875_pass_0934c646_0.txt
| ANP446_pass_90fea4ca_0.txt
| AOV830_pass_91b38c3f_78d37fb2_0.txt
+---w_1day
| FAW77777_pass_barcode08_8ca636cc_e83af5d2_0.txt
| FAW81892_pass_barcode01_f810f4db_dc345a2f_0.txt
| FAW81892_pass_barcode09_f810f4db_dc345a2f_0.txt
| FAW81892_pass_barcode14_f810f4db_dc345a2f_0.txt
+---w_2days
| FAW77777_pass_barcode09_8ca636cc_e83af5d2_0.txt
| FAW81892_pass_barcode02_f810f4db_dc345a2f_0.txt
| FAW81892_pass_barcode10_f810f4db_dc345a2f_0.txt
| FAW81892_pass_barcode15_f810f4db_dc345a2f_0..txt
+---w_3days
| FAW77777_pass_barcode10_8ca636cc_e83af5d2_0.txt
| FAW81892_pass_barcode03_f810f4db_dc345a2f_0.txt
| FAW81892_pass_barcode11_f810f4db_dc345a2f_0.txt
| FAW81892_pass_barcode16_f810f4db_dc345a2f_0.txt
+---w_5days
| FAW77777_pass_barcode11_8ca636cc_e83af5d2_0.txt
| FAW81892_pass_barcode04_f810f4db_dc345a2f_0.txt
| FAW81892_pass_barcode12_f810f4db_dc345a2f_0.txt
| FAW81892_pass_barcode17_f810f4db_dc345a2f_0.txt
+---w_8days
FAW77777_pass_barcode12_8ca636cc_e83af5d2_0.txt
FAW81892_pass_barcode07_f810f4db_dc345a2f_0.txt
FAW81892_pass_barcode13_f810f4db_dc345a2f_0.txt
FAW81892_pass_barcode18_f810f4db_dc345a2f_0.txt