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Diagnosis of prion diseases by RT-QuIC results in improved surveillance

Citation

Appleby, Brian (2020), Diagnosis of prion diseases by RT-QuIC results in improved surveillance, Dryad, Dataset, https://doi.org/10.5061/dryad.kwh70rz0g

Abstract

Objective:  We present the National Prion Disease Pathology Surveillance Center’s (NPDPSC) experience using cerebrospinal fluid (CSF) real time quaking induced conversion (RT-QuIC) as a diagnostic test, examine factors associated with false negative RT-QuIC results, and investigate RT-QuIC’s impact on prion disease surveillance. 

 

Methods:  Between May 2015-April 2018, the NPDPSC received 10,498 CSF specimens that were included in the study.   Sensitivity and specificity analyses were performed using 567 autopsy verified cases.  Prion disease type, demographic characteristics, specimen color, and time variables were examined for association with RT-QuIC results.  The effect of including positive RT-QuIC cases in prion disease surveillance was examined.

 

Results: The diagnostic sensitivity and specificity of RT-QuIC across all prion diseases was 90.3% and 98.5%, respectively.  Diagnostic sensitivity was lower for fatal familial insomnia, Gerstmann-Sträussler-Scheinker disease, sporadic fatal insomnia, variably protease sensitive prionopathy, and the VV1 and MM2 subtypes of sCJD.  Individuals with prion disease and negative RT-QuIC results were younger, had elevated tau levels, and non-elevated 14-3-3 levels compared to RT-QuIC positive cases. Sensitivity was high throughout the disease course.  Some cases that initially tested RT-QuIC negative had a subsequent specimen test positive.  Including positive RT-QuIC cases in surveillance statistics increased laboratory-based case ascertainment of prion disease by 90% over autopsy alone. 

 

Conclusions: RT-QuIC has high sensitivity and specificity for diagnosing prion diseases.  Sensitivity limitations are associated with prion disease type, age, and related CSF diagnostic results. RT-QuIC greatly improves laboratory-based prion disease ascertainment for surveillance purposes.  

 

Classification of Evidence:  This study provides Class III evidence that 2nd generation real time quaking-induced conversion (RT-QuIC) identifies prion disease with sensitivity of 90.3% and specificity of 98.5%, among patients being screened for these diseases due to concerning symptoms. 

Methods

Using STARD guidelines

Funding

Centers for Disease Control and Prevention, Award: NU38CK000480