Immigration of Sogatella furcifera from the Greater Mekong Subregion into northern China revealed by 2b-RAD sequencing
Cite this dataset
Dong, Zhaoke et al. (2020). Immigration of Sogatella furcifera from the Greater Mekong Subregion into northern China revealed by 2b-RAD sequencing [Dataset]. Dryad. https://doi.org/10.5061/dryad.kwh70rz1c
Abstract
Methods
Sogatella furcifera individuals were sampled in various geographic regions (China and Southeast Asia). These comprised six sites in Yunnan Province; four sites in Shandong Province; two sites in Laos, Cambodia, and Vietnam, respectively; and one site in Myanmar and Thailand, respectively. Samples were put in 95% ethanol and stored at -20°C until DNA extraction. Insect DNA was extracted using the TIAMamp Micro DNA Kit (Tiangen, Beijing, China) according to the manufacturer protocol. The 2b-RAD sequencing and genotyping were outsourced to Shanghai OE Biotech Ltd. (Shanghai, China). The genotype data contained information for each locus and each individual were obtained as vcf file.
Funding
Shandong Modern Agricultural Technology & Industry System, Award: SDAIT-17-07
National Natural Science Foundation of China, Award: 31860504
First Class Grassland Science Discipline Programme in Shandong Province of China
Key Research and Development Program of Yunnan Province, Award: 2019IB007