Data from: A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech
Data files
Oct 10, 2017 version files 3.29 GB
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Fig2.zip
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Fig3.zip
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Fig4.zip
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Fig5.zip
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functions.zip
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README_for_Fig2.txt
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README_for_Fig3.txt
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README_for_Fig4.txt
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README_for_Fig5.txt
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README_for_functions.txt
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README_for_Supplementary.txt
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Supplementary.zip
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Abstract
Studies of neuronal network emergence during sensory processing and motor control are greatly promoted by technologies that allow us to simultaneously record the membrane potential dynamics of a large population of neurons in single cell resolution. To achieve whole-brain recording with the ability to detect both small synaptic potentials and action potentials, we developed a voltage-sensitive dye (VSD) imaging technique based on a double-sided microscope that can image two sides of a nervous system simultaneously. We applied this system to the segmental ganglia of the medicinal leech Hirudo verbana. Double-sided VSD imaging enabled simultaneous recording of membrane potential events from almost all of the identifiable neurons. Using data obtained from double-sided VSD imaging we analyzed neuronal dynamics in both sensory processing and generation of behavior and constructed functional maps for identification of neurons contributing to these processes.