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Data from: A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech

Citation

Tomina, Yusuke; Wagenaar, Daniel A (2017), Data from: A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech, Dryad, Dataset, https://doi.org/10.5061/dryad.m20kh

Abstract

Studies of neuronal network emergence during sensory processing and motor control are greatly promoted by technologies that allow us to simultaneously record the membrane potential dynamics of a large population of neurons in single cell resolution. To achieve whole-brain recording with the ability to detect both small synaptic potentials and action potentials, we developed a voltage-sensitive dye (VSD) imaging technique based on a double-sided microscope that can image two sides of a nervous system simultaneously. We applied this system to the segmental ganglia of the medicinal leech Hirudo verbana. Double-sided VSD imaging enabled simultaneous recording of membrane potential events from almost all of the identifiable neurons. Using data obtained from double-sided VSD imaging we analyzed neuronal dynamics in both sensory processing and generation of behavior and constructed functional maps for identification of neurons contributing to these processes.

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Funding

National Science Foundation, Award: R01NS094403