Data from: Redox mediated carbon monoxide release from a manganese carbonyl—implications for physiological CO delivery by CORMs
Data files
Oct 07, 2021 version files 1.31 MB
Abstract
We evaluated the dynamics of hydrogen peroxide reactions with metal carbonyls. specifically a water-soluble manganese carbonyl. These files are the raw data for the quantitative mechanistic investigation of the H2O2 oxidation of the water-soluble model complex fac-[Mn(CO)3(Br)(bpCO2)]2–, (A, bpCO22– = 2,2’-bipyridine-4,4’-dicarboxylate dianion). The method of intial rates was utilized in the experimental studies thus raw spectral data for the figures and results presented in RSOS-211022 were included. These data demonstrated pH-dependent kinetics. All the raw absorbance data used to determine the initial reaction rates and observed rate constant from pH 6.1-7.4, 8-32 mM [H2O2]. and 19.5- 42oC are present. For soluble homogenous experiments, UV-Vis, Fluorescence assay, and FT-IR spectral data for conditions included in the manuscript. For insoluble products of the experiments, X-ray diffraction and ICP-AES raw data are included.
There are several untested hypotheses about the mechanisms of the reaction of manganese carbonyl with reactive oxygen species. We found that intracellular pH greatly influences the reaction rate and may be a control for redox-mediated CO release from mangense CORMs. Since the lysosome is more acidic and mitochondrial matrix is more basic than the cytosol it is likely different local rates of CO release would be observed. Furthermore, in considering the localization and diffusion of H2O2 in biological systems, it is likely that a relatively slow reaction with manganese carbonyls (compared to catalase) can exhibit physiological effects. The cytotoxic effects of Mn(I) carbonyls have been associated with the generation of hydroxyl radicals, however we founs no appreciable quantities of hydroxyl radicals compared to known catalysts. Thus we conclude that these cytotoxic effects are more likely due to released CO interacting with heme-proteins vital to cellular respiration. Our mechanistic and kinetic findings provide literature precedence for researchers examining physiological effects of Mn(I) carbonyls.
Methods
The data was collected using primarily UV-Vis scpectroscopy. Some of the data was collected using XRD, NMR, EPR, and IR spectroscopy; as well as GC-TCD and voltammetry.
Usage notes
The data can be used entirely as is. The analyses the authors used is presented in the manuscript and should enable someone to re-analyze this raw data similarly.
| ph7.4_8mMH2O2 | absorbance data at 408 nm for the reaction of A (at var. concentration) with H2O2 ([H2O2]total = 8 mM) at pH 7.4 and 36.8°C. |
| pH7.4_16mMH2O2 | absorbance data at 408 nm for the reaction of A (at var. concentration) with H2O2 ([H2O2]total = 16 mM) at pH 7.4 and 36.8°C. |
| pH7.4_32mMH2O2 |
absorbance data at 408 nm for the reaction of A (at var. concentration) with H2O2 ([H2O2]total = 32 mM) at pH 7.4 and 36.8°C. |
| pH6.1_8mMH2O2 | absorbance data at 408 nm for the reaction of A (at var. concentration) with H2O2 ([H2O2]total = 8 mM) at pH 6.1 and 36.8°C. |
| pH6.1_16mMH2O2 | absorbance data at 408 nm for the reaction of A (at var. concentration) with H2O2 ([H2O2]total = 16 mM) at pH 6.1 and 36.8°C. |
| pH6.1_32mMH2O2 | absorbance data at 408 nm for the reaction of A (at var. concentration) with H2O2 ([H2O2]total = 32 mM) at pH 6.1 and 36.8°C. |
| pH6.8_32mMH2O2 | absorbance data at 408 nm for the reaction of A (at var. concentration) with H2O2 ([H2O2]total = 32 mM) at pH 6.8 and 36.8°C. |
| pH7.1_32mMH2O2 | absorbance data at 408 nm for the reaction of A (at var. concentration) with H2O2 ([H2O2]total = 32 mM) at pH 7.1 and 36.8°C. |
| pH7.7_32mMH2O2 | absorbance data at 408 nm for the reaction of A (at var. concentration) with H2O2 ([H2O2]total = 32 mM) at pH 7.7 and 36.8°C. |
| pH8_32mMH2O2 | absorbance data at 408 nm for the reaction of A (at var. concentration) with H2O2 ([H2O2]total = 32 mM) at pH 8 and 36.8°C. |
| 19.5CvaryH2O2 | absorbance data at 408 nm for the reaction of A with H2O2 ([H2O2]total = variable) at pH 7.4 and 19.5°C. |
| 25.5CvaryH2O2 | absorbance data at 408 nm for the reaction of A with H2O2 ([H2O2]total = variable) at pH 7.4 and 25.5°C. |
| 31.5CvaryH2O2 | absorbance data at 408 nm for the reaction of A with H2O2 ([H2O2]total = variable) at pH 7.4 and 31.5°C. |
| 33.9CvaryH2O2 | absorbance data at 408 nm for the reaction of A with H2O2 ([H2O2]total = variable) at pH 7.4 and 33.9°C. |
| 42CvaryH2O2 | absorbance data at 408 nm for the reaction of A with H2O2 ([H2O2]total = variable) at pH 7.4 and 42°C. |
| UV-Vis FigS1 | UV-visible spectral data for fac-Mn(CO)3(Br)(bpCO2H) (90 µM) and bpCO2H (100 µM) in pH 7.4 phosphate buffered solution (0.118 M) at 25 °C. |
| 100mMpyridineFigureS16 | UV-visible spectral data recorded for a solution of A in pH 7.4 phosphate buffer before and after addition of pyridine. |
| 32mMH2O2afterpyrFigureS17 | UV-visible spectral data recorded for a solution of A substituted with pyridine in pH 7.4 phosphate buffer before and after addition of 32 mM H2O2 |
| XRDFigureS3 | X-ray diffraction of white solid precipitate collected from the reaction of A with H2O2 at in phosphate buffered solution |
| ICP | ICP-AES raw data of white solid precipitate collected from the reaction of A with H2O2 at in phosphate buffered solution |
| EmissionFigureS15 |
Emission spectral data of coumarin-3-carboxylic acid (345 µM) in phosphate buffer solution after a 3 h reactions under the following conditions: CuCl2 (45 µM) plus H2O2 (32 mM). A (45 µM) plus H2O2 (32 mM). H2O2 (32 mM) only. A (45 µM) only. CuCl2 (45 µM) only. (T = 25 °C). |
| EPRFigureS5-6 | Processed data for X-Band EPR spectrum of a phosphate buffered solution containing A and H2O2 |
| CVFigureS7-8 | Cyclic voltammograms of A in deaerated phosphate buffer solution scanned from 0 to 1.3V and from 0 to -1.6 V using a glassy carbon electrode and of a pH 7.4 solution of A and H2O2 in deaerated phosphate buffer at 37°C scanned from 0 to -0.85 V at different elapsed times after mixing indicating formation of O2 |
| IRFigureS10 | Temporal infrared spectral changes of a DMSO solution of A and H2O2 and 5% aqueous phosphate buffer at 25°C. |
Variables for the columns in the sheets are as follows.
Unless otherwise mentioned time values are in seconds, wavelength is in nm, and wavenumber is in cm-1
"EX 1" through "EX 117" in sheets titled pHX.X_YmMH2O2 are absorbance values.
"ligand" and "MnbpyCOOH" in sheet UV-vis FigS1 are absorbance values.
"blank" and "100mMPyr_1" through "100mMPyr_68" in sheet 100mMpyridineFigureS16 are absorbance values. "298 nm" is the absorbance value for all the data at that wavelength.
"32H2O2_100mMpyr_1" through "32H2O2_100mMpyr_100" in sheet 32mMH2O2afterpyrFigureS17 are absorbance values.
"degrees" in sheet XRDFigureS3 are the 2 θ angle whiel the "counts" are the number representing intensity.
The sheet ICP is the raw AES files. The wavelength and intensity of emission are reported. For examples 'Mn2576' had an emission intensity average(ave) = 328400 at wavelength 257.610 nm. The rest of the raw file is coded similarly.
In sheet EmissionFigureS15, "wl6" is in wavelengh. "{Mn}", "H2O2", "{Mn} + H2O2", "CuCl", and "Cu + H2O2" are all emission intensity counts.
"g" are magnetic field strength and all other columns are signl intensity in sheet EPRFigureS5-6.
In sheet CVFigureS7-8, any column title that starts "I_" is current im amps. The columbs titled "potential" are the applied potential in volts.
In sheet IRFigureS10, "WN1" through "WN15" are in cm-1 and "ABS1" through "ABS15" are the corresponding absorbance values.