Spreadsheets for determining the immunoreactivity of radioimmunoconjugates
Data files
Jan 03, 2024 version files 57.53 KB
Abstract
In the published manuscript for which this data set was created, we provide detailed protocols for the measurement of the immunoreactivity of radiolabeled antibodies. To provide some context, full-length antibodies and smaller antibody fragments have been viewed as promising vectors for the delivery of radionuclides to tumor tissues for decades. The creation of radioimmunoconjugates is predicated on the direct attachment of a radionuclide (i.e. a radiohalogen) or the initial bioconjugation of a chelator that can then be used to coordinate a radiometal. These two approaches have traditionally relied upon random reactions with residues within the immunoglobulin; however, stochastic modification strategies inevitably risk the integrity of the biomolecule itself and, as a result, can provide radioimmunoconjugates with subpar in vivo behavior. The initial evaluation of any novel radiolabeled antibody or antibody fragment should therefore involve the determination of the immunoconjugate's ability to bind its cognate antigen. These spreadsheets are companions to a manuscript in which we provide detailed protocols for three assays that can be used to determine the immunoreactivity of a radioimmunoconjugate: (1) a cell-based linear extrapolation assay; (2) a cell-based antigen saturation assay; and (3) a resin- or bead-based assay.
These are spreadsheets that accompany our guide to measuring the immunoreactivity of radioimmunoconjugates.
README
Summary of experimental efforts underlying this dataset
These are spreadsheets that accompany our guide to measuring the immunoreactivity of radioimmunoconjugates that appears in Molecular Imaging and Biology. The spreadsheets are currently filled with representative data collected according to the methods described in the Supplementary Materials that accompany the companion article in question. The spreadsheets are meant to be filled in with new raw data generated by readers of our protocols.
Description of file structure and contents
There are three Microsoft Excel spreadsheet files. The spreadsheets are currently filled with representative data collected according to the methods described in the Supplementary Materials that accompany the companion article in question. The spreadsheets are formatted in order to provide immunoreactivity values once users have filled them with data collected according to to the protocols described in the companion article in Molecular Imaging and Biology.
Definitions of all variables, abbreviations, missing data codes, and units
The following abbreviations are used. PBS = phosphate-buffered saline; CPM = counts-per-minute; Avg = Average; T/B = tube-to-background ratio; IR = immunoreactive fraction; %IR = percent immunoreactivity; std. deviation = standard deviation. There are no undefined variables, missing data codes, or missing units.
Links to other publicly accessible locations of the data
There are no links to other publicly accessible locations of the data.
Other sources, if any, that the data was derived from
There are no other sources of this data.
Any other details that may influence reuse or replication efforts
These issues are discussed at length in the companion article that is published in Molecular Imaging and Biology.