Data from: Enhanced competitive advantage of invasive plants by growth-defense trade-off: Evidence from phytohormone metabolism and transcriptomic analysis
Data files
Jan 28, 2025 version files 13.91 MB
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Data1_Plant_height.xlsx
12.84 KB
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Data2_Total_biomass.xlsx
12.78 KB
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Data3_Relative_growth_rate.xlsx
12.77 KB
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Data4_Auxin_content.xlsx
12.68 KB
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Data5_Gibberellin_content.xlsx
12.65 KB
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Data6_Jasmonic_acid_content.xlsx
12.69 KB
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Data7_Salicylic_acid_content.xlsx
12.71 KB
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Data8_Transcriptome.xlsx
13.80 MB
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Data9_qRT-PCR.xlsx
12.43 KB
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README.md
3.10 KB
Abstract
Invasive plants often exhibit a competitive advantage over native species, posing a serious threat to them. Phytohormone signaling can regulate the entire process of invasive plant growth and defense in ways that have largely unexplored for invaders in competitive conditions. Here, we planted an invasive species, Flaveria bidentis L., and three native species (Setaria viridis, Artemisia annua, and Xanthium sibiricum) as monocultures (two seedlings each) or mixtures (one F. bidentis with one native) in the greenhouse, then employed phytohormone metabolomics and transcriptomics to investigate the changes in hormone content and their gene expression patterns in F. bidentis during competitive processes with native plants. Our results revealed that during competition processes with three native species, the levels of growth-promoting hormones, auxin (IAA) and gibberellins (GA3), significantly increased in F. bidentis. These hormone increases were accompanied by the key up-regulation of genes in the auxin and gibberellin signaling pathways (e.g., ARF and GID1), which effectively spurred increases in plant height and biomass. Conversely, the levels of defense-related hormones, jasmonic acid (JA) and salicylic acid (SA), were significantly reduced, along with the down-regulation of genes in their signaling pathways, including COI1 and TGA. These decreases in defense-related hormones and gene expression alleviated the antagonistic effects on growth-promoting hormones, enabling F. bidentis to grow more rapidly and enhance its competitive advantage. Synthesis and applications. Our findings reveal that trade-offs between growth and defense hormone signaling pathways enhance the competitive advantage of invasive alien species over native ones. This insight into the invasive success of exotic species provides a foundation for developing targeted control strategies. By specifically regulating hormone signaling pathways, the competitiveness of invasive species could be dampened, thereby mitigating the threat to native ecosystems.
README: Enhanced competitive advantage of invasive plants by growth-defense trade-off: Evidence from phytohormone metabolism and transcriptomic analysis
https://doi.org/10.5061/dryad.mgqnk998t
Description of the data and file structure
This dataset contains nine .xlsx files, each focusing on different plant parameters. The files are named and structured as follows:
Files and variables
File:
File 1: Data1_Plant height : This file records plant height measurements
Variable: Plant Height
Unit: Centimeters (cm)
File 2: Data2_Total biomass: This file records plant biomass measurements
- Variable: Biomass
- Unit: Grams (g)
File 3: Data3_Relative growth rate: This file records plant growth rate measurements
- Variable: Growth Rate
- Unit: Milligrams per day (mg/day)
File 4: Data4_Auxin content: This file records plant auxin content
- Variable: Auxin content
- Unit: Nanograms per gram (ng/g)
File 5: Data5_Gibberellin content: This file records plant gibberellin content
- Variable: Gibberellin content
- Unit: Nanograms per gram (ng/g)
File 6: Data6_Jasmonic acid content: This file records plant jasmonic acid content
- Variable: Jasmonic content
- Unit: Nanograms per gram (ng/g)
File 7: Data7_Salicylic acid content: This file records plant salicylic acid content
- Variable: Salicyc content
- Unit: Nanograms per gram (ng/g)
File 8: Data8_Transcriptome: This file records plant gene expression
Variable: Gene Expression
Unit: Fragments Per Kilobase of transcript per Million mapped fragments (FPKM)
Column 1:Gene ID
Column 2-7: Gene expression (FPKM)
Column 8: log2FoldChange
Column 9: p-value
Column 10: Regulation
File 9: Data9_qRT-PCR: This file contains quantified gene expression levels measured by qRT-PCR
Variable: Gene Expression
Unit: Relative Quantification (2^(-ΔΔCt))
Description:
Abbreviations:
Fm: F. bidentis monoculture.
Sm: S. viridis monoculture.
Am: A. annua monoculture.
Xm: *X. sibiricum *monoculture.
F/F:S:F. bidentis in the mixture between F. bidenti*s and *S. viridis.
S/F:S: S. viridis in the mixture between F. bidentis and S. viridis.
F/F:A: F. bidentis in the mixture between F. bidentis and A. annua.
A/F:A: A. annua in the mixture between F. bidentis and A. annua.
F/F:X: F. bidentis in the mixture between F. bidentis and X. sibiricum.
X/F:X: X. sibiricum in the mixture between F. bidentis and X. sibiricum
The overall variables used in the dataset are as follows:
Plant height (cm)
Total biomass (g)
Relative growth rate (mg/day)
auxin content (ng/g)
Gibberellin content (ng/g)
Jasmonic acid content (ng/g)
Salicylic acid content (ng/g)
log2FoldChange of gene expression
Relative Expression ( 2^(-ΔΔCt))
Access information
Other publicly accessible locations of the data:
- No
Data was derived from the following sources:
- no