Data from: SK2 channels in cerebellar Purkinje cells contribute to excitability modulation in motor learning-specific memory traces

Grasselli, Giorgio, University of Chicago

Boele, Henk-Jan, Erasmus MC

Titley, Heather K., University of Chicago

Bradford, Nora, University of Chicago

van Beers, Lisa, Erasmus MC

Jay, Lindsey, University of Chicago

De Zeeuw, Chris I., Erasmus MC

Schonewille, Martijn, Erasmus MC

Hansel, Christian, University of Chicago, https://orcid.org/0000-0001-5750-7097

Beekhof, Gerco, Erasmus University Medical Center

Busch, Silas, University of Chicago

giorgio.grasselli@gmail.com, chansel@bsd.uchicago.edu

Published Dec 20, 2019 on Dryad. https://doi.org/10.5061/dryad.mh4f7n3

Cite this dataset

Grasselli, Giorgio et al. (2019). Data from: SK2 channels in cerebellar Purkinje cells contribute to excitability modulation in motor learning-specific memory traces [Dataset]. Dryad. https://doi.org/10.5061/dryad.mh4f7n3

Abstract

Neurons store information by changing synaptic input weights. In addition, they can adjust their membrane excitability to alter spike output. Here, we demonstrate a role of such ‘intrinsic plasticity’ in behavioral learning in a mouse model that allows us to detect consequences of absent excitability modulation, without alterations in synaptic plasticity. SK2-type, calcium-dependent K+ conductances are involved in excitability control as they contribute to the afterhyperpolarization (AHP) following spike bursts. SK2 channels are downregulated in activity-dependent intrinsic plasticity in cerebellar Purkinje cells. To study the relevance of excitability adjustment in cerebellar learning, we generated and tested mice with a Purkinje cell-specific SK2 knockout (L7-SK2). Deletion of SK2 channels enhanced Purkinje cell excitability and selectively prevented intrinsic plasticity. L7-SK2 mice showed impairment of eyeblink conditioning, but intact vestibulo-ocular reflex gain adaptation. Thus, cell-autonomous plasticity of membrane excitability can be isolated from synaptic plasticity and is essential for specific learned motor behaviors.

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