Data from: Genome scan identifies flowering-independent effects of barley HsDry2.2 locus on yield traits under water deficit

Merchuk-Ovnat, Lianne, Agricultural Research Organization

Silberman, Roi, Hebrew University of Jerusalem

Laiba, Efrat, Agricultural Research Organization

Maurer, Andreas, Martin Luther University Halle-Wittenberg

Pillen, Klaus, Martin Luther University Halle-Wittenberg

Faigenboim, Adi, Agricultural Research Organization

Fridman, Eyal, Agricultural Research Organization

Published Jan 10, 2019 on Dryad. https://doi.org/10.5061/dryad.mr070

Cite this dataset

Merchuk-Ovnat, Lianne et al. (2019). Data from: Genome scan identifies flowering-independent effects of barley HsDry2.2 locus on yield traits under water deficit [Dataset]. Dryad. https://doi.org/10.5061/dryad.mr070

Abstract

Increasing crop productivity under climate change requires the identification, selection and utilization of novel alleles for breeding. We analyzed the genotype and field phenotype of the barley HEB-25 multi-parent mapping population under well-watered and water-limited (WW and WL) environments for two years. A genome-wide association study (GWAS) for genotype by-environment interactions was performed for ten traits including flowering time (HEA) and plant grain yield (PGY). Comparison of the GWAS for traits per-se to that for QTL-by-environment interactions (QxE), indicates the prevalence of QxE mostly for reproductive traits. One QxE locus on chromosome 2, Hordeum spontaneum Dry2.2 (HsDry2.2), showed a positive and conditional effect on PGY and grain number (GN). The wild allele significantly reduced HEA, however this earliness was not conditioned by water deficit. Furthermore, BC2F1 lines segregating for the HsDry2.2 showed the wild allele confers an advantage over the cultivated in PGY, GN and harvest index as well as modified shoot morphology , longer grain filling period and reduced senescence (only under drought), therefore suggesting adaptation mechanism against water deficit other than escape. This study highlights the value of evaluating wild relatives in search of novel alleles and clues to resilience mechanism underlying crop adaptation to abiotic stress.

Usage notes

LOM et al JXB_PHENO_GENO-2

Genotypic and phenotypic data for the HEB-25 barley mapping population grown under well-watered (WW) and water-limited (WL) environments for two years (2015 and 2016). The genotypic data: 11, homozygous for the cultivated Hordeum vulgare allele; 12, heterozygous; 22, homozygous for the wild donor (H. spontaneum). Phenotype: Heading time (HEA), defined as the time between sowing to time at which the first spike of 50% of the plants in a plot reaches BBCH49 (first awns visible), was recorded based on daily inspection. Days from sowing to stage BBCH87 (hard dough: grain content solid: fingernail impression held) was recorded as maturity (MAT). At maturity, plant height (HEI) was measured from the soil surface to the base of the three first spikes per plot. At full grain maturity and after plants were fully dried, all aboveground biomass was harvested and weighed to determine total dry matter (TDM). Notably, all the free-thrashing material (app. ¼ of the material) was caged between BBCH49 and BBCH87 to avoid loss of spikes. Spikes were then threshed and weighed to determine plant grain yield (PGY). Finally, grains were counted to estimate grain number per mini-plot (GN) and average grain weight (GW). Harvest index (HI) was calculated as the ratio between PGY and TDM. Vegetative dry matter (VDW) was calculated by subtracting PGY from TDM. The grain-filling period (GFP) was calculated by subtracting HEA from MAT. Trait values were adjusted based on the ratio between population mean values in the two years. The adjusted HEB means across years were used in the GWAS.

Location

54m above sea level

E34°47′

Rehovot

Israel

N31°54′