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Dryad

The 153 readable DNA sequences of a diet research on Eurasian otter of Kinmen Island

Data files

This dataset is embargoed . Please contact Nian-Hong Jang-Liaw at moc.liamg@wt.ocat with any questions.

Lists of files and downloads will become available to the public when released.

Abstract

It is well known that Eurasian otters principally feed on fishes and crustaceans, however, their detailed diet taxonomies are not fully understood. I performed a series of Sanger-sequencing reactions, utilizing nine primer sets for Eurasian otter diet research. In these data materials, in total 153 readable DNA sequences of a diet research on Eurasian otter of Kinmen Island are presented. Such information provides a very detailed insight of feeding behavior of the Eurasian otter in Kinmen.

These sequences were sequenced from 49 DNA samples extracted from fresh spraints collected from Kinmen from April 2017 to November 2018. Each DNA sample was PCR-amplified 9 times and with 9 primer sets. Eight of them are published universal COI primers and targeted toward different prey taxa. Besides, I designed a group of COIII primers for the introduced tilapia, the most abundant prey species in Kinmen Island.

In total, 16 prey types were identified across all 49 samples. Furthermore, another identified sequence group comprised “non-food species”, including bacteria, amoeba, diatoms, small insects (e.g. ants and mosquitos) and small invertebrates, as well as the otters’ sequences themselves. The detailed information of these 153 sequences including collection sites, primers, identified food/non-food species, linkage of blast results, etc. are listed in S2.xlxs; for the sequences themselves are listed in S3.txt.