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A green method of extracting and recovering flavonoids from Acanthopanax senticosus using deep eutectic solvents

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Aug 25, 2021 version files 673.19 KB

Abstract

In recent years, green extraction of bioactive compounds from herbal medicines has generated widespread interest. Deep eutectic solvents (DES) have widely replaced traditional organic solvents in the extraction process. In this study, the efficiencies of eight tailor-made DESs in extracting flavonoids from Acanthopanax senticosus (AS) were compared. Response surface methodology (RSM) was employed to optimize the influencing parameters including ultrasonic power, HBA-HBD ratio, water content, solid-liquid ratio, extraction temperature and extraction time. DES composed of glycerol and levulinic acid at a 1:1 ratio was established as the most suitable extraction medium. Optimal conditions were ultrasonic power of 500W, water content of 28%, solid-liquid ratio of 1:18 g·mL-1, extraction temperature of 55℃ and extraction time of 73 minutes. The extraction yield of AS total flavonoids reached 23.928±0.071 mg·g-1, which was 86.3% and 43.8% higher compared with traditional solvent soak and ethanol reflux extraction methods, respectively. Macroporous resin (D-101, HPD-600, S-8 and AB-8) was used to recover flavonoids from extracts. The AB-8 resin showed higher adsorption/desorption performance, with a recovery rate of total flavonoids of up to 71.56±0.256%. In addition, DES solvent could be efficiently recovered through this process and reused. In summary, ultrasonic-assisted DES combined with the macroporous resin enrichment method is exceptionally effective in extracting flavonoids from AS and provides a promising environmentally friendly and recyclable strategy for flavonoid extraction from natural plant sources.