We examined whether warming, acidification, and different food availability regimes interacted to affect the survival, development, and growth of larval crown-of-thorns starfish, Acanthaster sp. (CoTS). Larvae were reared in all combinations of two temperatures (26, 30 °C), two pH levels (pH 8.0, 7.6), and three food regimes (‘low’ ration: 1,000 cells mL^-1; ‘switch’ ration: 1,000 cells mL^-1 until day 11, followed by 50,000 cells mL^-1; and ‘high’ ration: 50 000 cells mL^-1). 

See Methods section of the related paper "Variable food alters responses of larval crown-of-thorns starfish to ocean warming but not acidification" Communication Biology for details.

Mos, B., Mesic, N. & Dworjanyn, S.A. Variable food alters responses of larval crown-of-thorns starfish to ocean warming but not acidification. Commun Biol 6, 639 (2023). https://doi.org/10.1038/s42003-023-05028-1

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