Drosophila melanogaster wounding in the wild

https://doi.org/10.5061/dryad.mw6m90640

The dataset consists of two data files, one containing information on wounding on individual wild-collected Drosophila melanogaster adult flies, the other file contains information on mites that were found on individual wild-collected Drosophilidae flies.

Description of the data and file structure

Note that in the csv files and names, the following abbreviations are used:

Combined damage HTLA : Combined damage found on the head, thorax, legs and abdomen

1. File List:

damage.R The R code required to process the data in the csv files and produce the data figures for the study

damage_data.csv Wounding found on individual wild-collected flies

mite.csv Mite infestations on individual wild-collected flies

mites_prob.csv Number of mite infestation on wild-collected flies across sex, season and sites

2. Relationship between files, if important: The data that is presented in the two csv files was collected from the same wild fly collections. These collections were carried out in 2021, across three seasons and three sites in Berlin and Brandenburg.

3. Are there multiple versions of the dataset? no

METHODOLOGICAL INFORMATION

1. Description of methods used for collection/generation of data:

The fly and data collection was carried out in the methods described in How frequently are insects wounded in the wild? A case study using Drosophila melanogaster.

2. Methods for processing the data: This information is given in the accompanying .R script.

3. Instrument- or software-specific information needed to interpret the data:

No specific software is needed to interpret the data.

4. Describe any quality-assurance procedures performed on the data:

At data entry, the data was double-checked for accuracy.

DATA-SPECIFIC INFORMATION FOR: damage_data.csv

1. Number of variables: 27

2. Number of cases/rows: 1246

3. Variable List:

Each row contains data for an individual fly and indicates the presence or absence of various types of wounding or damage

site Location of the fly collection

season Time of the fly collection. 1 = Early summer (24th June-21st July), 2 = Late summer (24th August-6th September), 3 = Autumn (4th-8th October).

sample_id Identification number of the fly

sex Sex of the fly. f = Female, m = Male.

species Species of the fly. dmel = Drosophila melanogaster, dsim = Drosophila simulans.

head_dmg Whether the fly had head damage or not. 1 = yes, 0 = no.

missing_leg Whether the fly had leg damage or not. 1 = yes, 0 = no.

black_spots_on_legs Whether the fly had melanized black spots on the legs or not. 1 = yes, 0 = no.

total_leg_dmg Whether the fly had any damage and/or melanized spots on the legs or not. 1 = yes, 0 = no.

thorax_black_spots Whether the fly had melanized black spots on the thorax or not. 1 = yes, 0 = no.

ventral_abdomen_black_spots Whether the fly had melanized black spots on the ventral abdomen or not. 1 = yes, 0 = no.

dorsal_abdomen_black_spots Whether the fly had melanized black spots on the dorsal abdomen or not. 1 = yes, 0 = no.

total_abdomen_black_spots Whether the fly had melanized black spots on the ventral and/or dorsal abdomen or not. 1 = yes, 0 = no.

all_dmg_presence_absence_no_parasitoid Whether the fly had any type of damage/wound or not. Absence or presence of parasitoid not included. 1 = yes, 0 = no.

all_dmg_sum_no_parasitoid Total number of damage/wound had found on the fly. Absence or presence of parasitoid not included.

notches_on_veins_total Whether the fly had notches in an area with veins on either the left or/and right side of the wing or not. 1 = yes, 0 = no.

notches_not_on_veins_total Whether the fly had notches in an area without veins on either the left or/and right side of the wing or not. 1 = yes, 0 = no.

area_on_veins_total Whether the fly had area in an area with veins on either the left or/and right side of the wing or not. 1 = yes, 0 = no.

area_not_on_veins_total Whether the fly had area in an area without veins on either the left or/and right side of the wing or not. 1 = yes, 0 = no.

tear_on_veins_total Whether the fly had tear in an area with veins on either the left or/and right side of the wing or not. 1 = yes, 0 = no.

tear_not_on_veins_total Whether the fly had tear in an area without veins on either the left or/and right side of the wing or not. 1 = yes, 0 = no.

wing_dmg_on_veins Whether the fly had any wing damage an area with veins or not. 1 = yes, 0 = no.

wing_dmg_not_on_veins Whether the fly had any wing damage an area without veins or not. 1 = yes, 0 = no.

wing_dmg_total Whether the fly had any type of wing damage or not. 1 = yes, 0 = no.

copulatory_wounds Whether the fly had copulatory wounds or not. 1 = yes, 0 = no.

parasitoid Whether the fly had any encapsulated parasitoids or not. 1 = yes, 0 = no.

number_parasitoid Total number of melanised patches (encapsulated parasitoids) under the fly cuticle.

4. Missing data codes:

Missing data are denoted as “NA”

DATA-SPECIFIC INFORMATION FOR: mite_data.csv

1. Number of variables: 10

2. Number of cases/rows: 56

3. Variable List:

Each row contains data for an individual fly and indicates the presence or absence of various types of wounding or damage

site Location of the fly collection.

season Time of the fly collection. 1 = Early summer (24th June-21st July), 2 = Late summer (24th August-6th September), 3 = Autumn (4th-8th October).

fly_sample_id Identification number of the fly.

fly_species Species of the fly.

fly_sex Sex of the fly. f = Female, m = Male.

melanized_spots Whether the fly had melanised black spots after removing the mite from the fly body or not. 1 = yes, 0 = no.

mite_sample_id Identification number of the mite.

mite_species Species of the mite.

attachment_place Mite attachment place on the fly body.

number_of_mites Total number of mites on the fly.

4. Missing data codes:

Missing data are denoted as “NA”

DATA-SPECIFIC INFORMATION FOR: mite_prob.csv

1. Number of variables: 10

2. Number of cases/rows: 56

3. Variable List:

Each row contains data for an individual fly and indicates the presence or absence of various types of wounding or damage

site Location of the fly collection.

season Time of the fly collection. 1 = Early summer (24th June-21st July), 2 = Late summer (24th August-6th September), 3 = Autumn (4th-8th October).

sex Sex of the fly.

yes_mites The number of flies found with a mite attached to their body.

no_mites The number of flies not found with a mite attached to their body.

4. Missing data codes:

No missing data.

Sharing/Access information

NA

Code/Software

R code needed for analyses and figures as shown in the manuscript, can be found in the file: damage.R