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Pigmentation Genes Show Evidence of Repeated Divergence and Multiple Bouts of Introgression in Setophaga Warblers

Citation

Toews, David et al. (2020), Pigmentation Genes Show Evidence of Repeated Divergence and Multiple Bouts of Introgression in Setophaga Warblers, Dryad, Dataset, https://doi.org/10.5061/dryad.n02v6wwvv

Abstract

Species radiations have long served as model systems in evolutionary biology. However, it has only recently become possible to study the genetic bases of the traits responsible for diversification, and only in a small number of model systems. Here we use genomes of 36 species of North, Central, and South American warblers to highlight the role of pigmentation genes—involved in melanin and carotenoid processing—in the diversification of this group. We show that agouti signaling protein (ASIP) and beta-carotene oxygenase 2 (BCO2) are predictably divergent between species that differ in the distribution of melanin and carotenoid in their plumages, respectively. Among species, sequence variation at ASIP broadly mirrors the species' phylogenetic history, consistent with repeated, independent mutations generating melanin-based variation. In contrast, BCO2 variation is highly discordant from the species tree, with evidence of cross-lineage introgression among species like the yellow warbler (Setophaga petechia) and magnolia warbler (S. magnolia) with extensive carotenoid-based coloration. We also detect introgression of a small part of the BCO2 coding region (<3 kb) in S. discolor and S. vitellina, including an amino acid substitution that is unique to warblers but otherwise highly conserved across birds. Lateral transfer of carotenoid-processing genes has been documented in arthropods, but introgression of BCO2 as demonstrated here—presumably adaptive—represents the first example of carotenoid gene transfer among vertebrates. These contrasting genomic patterns show that both independent evolution in a common set of genes and past gene flow have fueled plumage diversification in this colorful avian radiation.

Methods

See supplemental methods for how the genomic data was collected.

Usage Notes

Information for samples sequenced for the study, as well as NCBI Accession numbers for each sample. 

Column heading information:

  • Genus & Species: latin names based off Lovette et al. (2010; doi:10.1016/j.ympev.2010.07.018).
  • ID: the ID unique ID number given to the sample. Note that the "filename" for the reads uploaded to NCBI may differ from this sample name.
  • ID_type: either "museum voucher" (obtain additional sample information from the museum identified), "USGS_band" (additional locality and sampling information can be obtained from the USGS bird banding database), or "project_ID" (samples collected for unique projects on rare birds; contact the collector for additional sample information).
  • Museum or Collector: The identifier of the museum or collector that contributed the sample.
  • Institution: Additional information if the museum or collector was associated with a broader institution (such as a University).
  • Accession: This is the NCBI accession number.
  • Additional_ID: For CUMV samples this represents the additional "tissue ID" value associated with the sample. For blood samples collected by "David Toews" these represent unique record IDs, in addition to the band number.
  • Original publication: DOI for which publication was the data for each sample was first associated with.

Funding

Pennsylvania State University

Huck Institutes of the Life Sciences

Cornell Lab of Ornithology

Eberly College of Science