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Major oscillations in spontaneous home‑cage activity in C57BL/6 mice housed under constant conditions

Cite this dataset

Ulfhake, Brun; Rullman, Eric; Pernold, Karin (2021). Major oscillations in spontaneous home‑cage activity in C57BL/6 mice housed under constant conditions [Dataset]. Dryad.


The mouse is the most important mammalian model in life science research and the behavior of the mouse is a key read-out of experimental interventions and genetic manipulations. To serve this purpose a solid understanding of the mouse normal behavior is a prerequisite. Using 14–19 months of cumulative 24/7 home-cage activity recorded with a non-intrusive technique, evidence is here provided for a highly significant circannual oscillation in spontaneous activity (1–2 SD of the mean, on average 65% higher during peak of highs than lows; P = 7E−50) of male and female C57BL/6 mice held under constant conditions. The periodicity of this hitherto not recognized oscillation is in the range of 2–4 months (average estimate was 97 days across cohorts of cages). It off-sets responses to environmental stimuli and co-varies with the feeding behavior but does not significantly alter the preference for being active during the dark hours. The absence of coordination of this rhythmicity between cages with mice or seasons of the year suggest that the oscillation of physical activity is generated by a free-running intrinsic oscillator devoid of external timer. Due to the magnitude of this rhythmic variation it may be a serious confounder in experiments on mice if left unrecognized.


Collected via DVC from home-cage. Raw data from the 12 electrodes are averaged across electrode per min. Data covers +14 months of activity in 14 cages collected in 3 csv files.

Usage notes

All 10 data sets (one for each cage used) arranged in the three groups are available as three comma delimited csv files at: Dryad (

All data files have the following column headings for each cage:

Example from file CC III




dt (local)





2016-11-23 09:19





2016-11-23 09:20



Where board is the cage ID, GlobalAve is the average number of activations min-1 across all twelve electrodes, age is the age of the animals inside the cage, dt is local date followed by time stamp (CET), and n_count is number of samples min-1.

Lights-on and Lights-off, respectively, happened every day at 04:00 (h:min; zeitgeber time 0) and 16:00 (h:min; zeitgeber time 12) dt (local).


Karolinska Institutet