Heatwave resilience of juvenile white sturgeon is associated with epigenetic and transcriptional alterations
Data files
Sep 19, 2023 version files 20.86 KB
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CTMax_data.csv
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DNA_methylation_data.csv
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hypoxia_data.csv
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mRNA_data.csv
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README.md
Abstract
Heatwaves are increasing in frequency and severity, posing a significant threat to organisms globally. In aquatic environments, heatwaves are often associated with low environmental oxygen, which is a deadly combination for fish. However, surprisingly little is known about the capacity of fishes to withstand these interacting stressors. This issue is particularly critical for species of extreme conservation concern such as sturgeon. We assessed the tolerance of juvenile white sturgeon from an endangered population to heatwave exposure and investigated how this exposure affects tolerance to additional acute stressors. We measured whole-animal thermal and hypoxic performance and underlying epigenetic and transcriptional mechanisms. Sturgeon exposed to a simulated heatwave had increased thermal tolerance and exhibited complete compensation for the effects of acute hypoxia. These changes were associated with an increase in mRNA levels involved in thermal and hypoxic stress (hsp90a, hsp90b, hsp70 and hif1a) following these stressors. Global DNA methylation was sensitive to heatwave exposure and rapidly responded to acute thermal and hypoxia stress over the course of an hour. These data demonstrate that juvenile white sturgeon exhibit substantial resilience to heatwaves, associated with improved cross-tolerance to additional acute stressors and involving rapid responses in both epigenetic and transcriptional mechanisms.
README: Heatwave resilience of juvenile white sturgeon is associated with epigenetic and transcriptional alterations
Data associated with the article - Heatwave resilience of juvenile white sturgeon is associated with epigenetic and transcriptional alterations at Scientific Reports: https://doi.org/10.1038/s41598-023-42652-7.
Data files description
- CTMax data This file contains data on maximum thermal tolerance of white sturgeon prior to and after the heatwave at both 70% and 100% air saturation
- Hypoxia data This file contains data on the hypoxia tolerance (oxygen% at the time of LOE) of white sturgeon prior to and after the heatwave at 13C, 17C, and 20C.
- mRNA data This file contains mRNA levels in two tissues (gill and heart) for four genes, HSP90a, HSP90b, HSP70, and HIF1a in control samples (Day 0, Day 10, and Day 20) and post-acute stressor (CTMax or Hypoxia trials). Data are expressed as deltadeltaCT and normalized to reference genes and the control group (Day 0, non-stressed fish).
- Global DNA methylation This file contains data on the DNA methylation % in two tissues (gill and heart) in control samples (Day 0, Day 10, and Day 20) and post-acute stressor (CTMax or Hypoxia trials).
Description of the variables present in the data files:
- Sample order/number: the order the fish were sampled during a trial or run in an assay
- Treatment: the respective acclimation treatment (Day 0, Day 10, or Day 20)
- Fish ID: the unique fish number identifier
- DO(%) during trial: the dissolved oxygen % saturation during the CTMax trial (70 or 100%)
- CTMax (C): the temperature at which the fish LOE
- Temperature during trial (C): the temperature during hypoxia trials (13, 17, or 20C)
- DO% at LOE: the dissolved oxygen % at the time the fish LOE
- Tissue: the tissue used, gill or heart
- Control or trial: whether the sample was a control (baseline) sample or a post-trial sample (CTMax or hypoxia)
- -HSP90a, HSP90b, HSP70, HIF1a: these are the genes measured in the study
- -DNA methylation %: this refers to the % methylation of a given tissue for control samples or post-trial samples