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4D light sheet imaging, computational reconstruction, and cell tracking in mouse embryos -- example data (raw .czi and fused .klb light sheet images of mouse E7.5)

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Dec 12, 2024 version files 26.05 GB

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Abstract

This dataset contains example .czi (Zeiss Z.1 Lightsheet format) image files and corresponding and multi-view fused .klb dataset, containing 3 hours of time lapse imaging of a mouse embryo at E7.5 / EHF, for processing and analysis. To be published with "4-dimension light sheet imaging and cell tracking in mouse embryos" in STAR Protocols. The dataset is intended for users to rehearse our comprehensive workflow for in toto capture, processing, and analysis of multi-view LSFM experiments using the ex vivo mouse embryo as a model system of development. Our protocol describes imaging on commercial LSFM instruments, and recites computational analysis in discrete and accessible chunks, using open source software.