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Cytb gene sequences of Fejervarya species from Lesser Sunda, Indonesia and other Asian countries

Citation

Hasan, Mahmudul (2022), Cytb gene sequences of Fejervarya species from Lesser Sunda, Indonesia and other Asian countries, Dryad, Dataset, https://doi.org/10.5061/dryad.np5hqbzxf

Abstract

Cyt b gene sequences of Fejervarya species were done and later submitted to DDBJ. Then, we received accession number which are used in our manuscript "Postmating isolation and evolutionary relationships among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by crossing experiments and mtDNA Cytb sequences analyses". These are genuine data. There is no conflict of interest of these data.

Methods

A total of 27 frogs belonging to the genus Fejervarya were used for genetic research. Genomic DNA for PCR was extracted from clipped toes using the DNAeasy Tissue Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions. The extracted DNA solutions were used to amplify a partial region of the Cytb gene corresponding to nucleotide positions 14396–15063 in the Hoplobatrachus tigerinus complete mtDNA sequence (accession no. AP011543, Alam et al. 2010). PCR amplification and sequencing were performed using the primers Fow-1-1 and Rev-1 (Hasan et al. 2012) to obtain a fragment of ca. 667 bp. The resultant nucleotide sequences were aligned using the ClustalW program (Thompson et al. 1994). A phylogeny was constructed using the maximum likelihood (ML) method implemented in Treefinder (Jobb 2008) and branch support was evaluated by a bootstrap analysis with 100 replicates. The resultant sequence data were deposited in the DDBJ database under accession nos. LC706527–LC706542. Additional Cytb data were obtained from GenBank for tree construction.

Funding

Ministry of Education, Japan, Award: 17570082

Ministry of Education, Japan, Award: 20510216