Graphene oxide (GO) is a derivative of graphene nanosheet which is the most promising material of the decade in biomedical research. In particular, it has been known as an antimicrobial nanomaterial with good biocompatibility. In this study, we have synthesized and characterize GO and checked its antimicrobial property against different Gram-negative and Gram-positive multi drug resistant (MDR) hospital superbugs grown in solid agar-based nutrient plates with and without human serum through the utilization of agar well diffusion method, live/dead fluorescent staining and genotoxicity analysis. The main focus of the antimicrobial activity analysis is to distinguish the killing performance of GO in artificial and blood containing media because after injection into the bloodstream the activity of GO may be modified by adsorption of blood proteins or other biomolecules. No significant changes in antibacterial activity were found in these two different conditions. We also compare the bactericidal capability of GO with some commonly administrated antibiotics and in all cases the degree of inhibition is found to be higher. The data presented here are novel and show that GO is an effective bactericidal agents against different superbugs and can be used as a future antibacterial agent.

Spectroscopic Characterization of GO

1.The diluted aqueous solutions of GO were taken in crystal cuvette and placed in Hitachi (U-2001, Tokyo, Japan) for UV-vis measurement. The cuvettes were out of all kinds of finger print and spot to get accurate result. DI water was used as reference for all the tests.

2. Ringaku Mini Flex, 600, Japan was used to analyze XRD at 40 mA and 40 kV within the range of 2θ = 5–50° using Copper K-α radiation.

3. In Raman spectroscopy an invia laser-Raman spectrometer (The Renishaw, UK) containing 514 argon ion laser was run.

4. Nicolet NEXUS 470 was applied to obtain FTIR spectra of graphite and GO. KBr was used to prepare sample pellets for the experiment.

5. The TGA was conducted by Shimadzu TG 50 instrument, Japan at 10 °C min ^-1 heating rate between 25-700°C temperature range. 

All the experiments were conducted several times util get the best results. All the data were collected form the machines by CD and export to my computer. Finally the graphs were  prepared by microsoft excel and put to the manuscript.

 

Everything is ready to use.