Characterization of Salix nigra floral insect community and activity of three native Andrena bees
DiFazio, Stephen et al. (2022), Characterization of Salix nigra floral insect community and activity of three native Andrena bees, Dryad, Dataset, https://doi.org/10.5061/dryad.nvx0k6drr
Data was collected using a combination of specimen collection with hand trapping, visual observations, and pan traps as described in "The very handy manual: how to catch and identify bees and manage a collection" (https://www.usgs.gov/media/files/how-catch-and-identify-bees-and-manage-a-collection). Floral VOCs were collected using a using a dynamic headspace method with nylon oven bags which had polytetrafluoroethylene (PTFE) ports that were fixed and connected to chemical traps consisting of 65 mm long and 3 mm internal diameter glass tubes packed with 20 mg of Super Q adsorbent (80/100 mesh size, DVB/ ethylvinylbenzene polymer, Alltech Associates Inc., Deerfield, IL, USA). VOC samples were then analyzed with a Thermo Trace 1310 GC coupled to a Thermo ISQ MS with electron ionization (EI) at 70.0 eV at 250 °C. Phenolic glycoside chemistry data was obtained from flash frozen floral and leaf tissue analyzed on UHPLC [Waters Acquity I-Class UPLC with a photodiode array detector (PDA) and a 3100 SQ mass spectrometer (MS), Milford, MA, USA] with commercially available standards of salicin (Sigma-Aldrich), and salicortin, tremuloidin, and tremulacin that had been previously isolated and purified from aspen foliage.
Data is ideal for looking at relationships between insect composition and chemistry composition using mantel tests, but total volatile output could be compared among subsequent years to determine how changing temperatures in early season may influence volatilization of catkin scent and overall insect activity on flowers.
National Science Foundation, Award: 1542509