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Data from: A novel technique to develop thoracic spinal laminectomy and a methodology to assess the functionality and welfare of the contusion SCI rat model

Citation

Harikrishnan, Vijayakumar Sreelatha; Lissy, Kalyana Krishnan; Abelson, Klas Stig Peter (2019), Data from: A novel technique to develop thoracic spinal laminectomy and a methodology to assess the functionality and welfare of the contusion SCI rat model, Dryad, Dataset, https://doi.org/10.5061/dryad.p1m7167

Abstract

This study reports the advantage of a novel technique to employ motorised dental burr to assist laminectomy over the conventional manual technique at T11-T12 vertebra level in rat model. Twenty four female rats were randomly assigned to 4 groups: (1) conventionally-laminectomised (2) dental burr assisted laminectomised (3) conventionally-laminectomised with spinal cord contusion and (4) dental burr assisted laminectomised with spinal cord contusion. Postoperative bodyweights, rat grimace scale (RGS), open cage activity, rearing and Basso Beattie Bresnahan (BBB) score was studied at 1, 7, 14, 21 and 28th postoperative day and area of spinal tissue affected was evaluated histologically. Laminectomised and spinal cord injured rats from dental burr groups showed significantly more weight gain and less weight loss respectively in comparison with respective conventionally-laminectomised groups at various time points. Significantly higher RGS score was noticed in conventionally-laminectomised animals on day one in comparison to the burr assisted laminectomy and presence of pain was evident until 14th day in the conventionally spinal injured group. The BBB score did not differ between groups, whereas laminectomy groups showed more resting time than spinal injury groups. Dental burr assisted laminectomy group showed consistently more overall rearing score in comparison to conventional-laminectomy group at all time points. This study suggests that the use of dental burr assisted technique to perform laminectomy will bring refinement by producing less pain, aiding in better recovery, removing procedural artifacts without affecting the outcome of the model and may adopted elsewhere for better welfare.

Usage Notes

Location

India