Plant diversity and soil legacy independently affect the plant metabolome and induced responses following herbivory
Data files
Oct 17, 2023 version files 1.26 MB
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Constitutive_Metabolome_Ger_pra_PPI_leaves.csv
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Constitutive_Metabolome_Ger_pra_PPI_roots.csv
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Constitutive_Metabolome_Ger_pra_PSI_leaves.csv
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Constitutive_Metabolome_Ger_pra_PSI_roots.csv
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Constitutive_Metabolome_Leu_vul_PPI_leaves.csv
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Constitutive_Metabolome_Leu_vul_PPI_roots.csv
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Constitutive_Metabolome_Leu_vul_PSI_leaves.csv
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Constitutive_Metabolome_Leu_vul_PSI_roots.csv
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Constitutive_Metabolome_Meta-Data_PPI.csv
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Constitutive_Metabolome_Meta-Data_PSI.csv
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Constitutive_Metabolome_Ran_acr_PPI_leaves.csv
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Constitutive_Metabolome_Ran_acr_PPI_roots.csv
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Constitutive_Metabolome_Ran_acr_PSI_leaves.csv
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Constitutive_Metabolome_Ran_acr_PSI_roots.csv
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Herbivore-induced_Metabolome_Ger_pra_PPI.csv
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Herbivore-induced_Metabolome_Ger_pra_PSI.csv
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Herbivore-induced_Metabolome_Leu_vul_PPI.csv
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Herbivore-induced_Metabolome_Leu_vul_PSI.csv
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Herbivore-induced_Metabolome_Meta-Data_PPI.csv
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Herbivore-induced_Metabolome_Meta-Data_PSI.csv
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Herbivore-induced_Metabolome_Ran_acr_PPI.csv
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Herbivore-induced_Metabolome_Ran_acr_PSI.csv
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README.md
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Abstract
Plant and soil biodiversity can have significant effects on herbivore resistance mediated by plant metabolites. Here, we disentangled the independent effects of plant diversity and soil legacy on constitutive and herbivore-induced plant metabolomes of three plant species in two complementary microcosm experiments.
First, we grew plants in sterile soil with three different plant diversity levels. Second, single plant species were grown on soil with different plant diversity-induced soil legacies. We infested a subset of all plants with Spodoptera exigua larvae, a generalist leaf-chewing herbivore, and assessed foliar and root metabolomes.
Neither plant diversity nor soil legacy had significant effects on overall foliar, root, or herbivore-induced metabolome composition. Herbivore-induced metabolomes, however, differed from those of control plants. We detected 139 significantly regulated metabolites by comparing plants grown in monocultures with conspecifics growing in plant or soil legacy mixtures. Moreover, plant-plant and plant-soil interactions regulated 141 metabolites in herbivore-induced plants.
Taken together, plant diversity and soil legacy independently alter the concentration and induction of plant metabolites, thus affecting the plant’s defensive capability. This is a first step towards disentangling plant and soil biodiversity effects on herbivore resistance, thereby improving our understanding of the mechanisms that govern ecosystem functioning.
README: Plant diversity and soil legacy independently affect the plant metabolome and induced responses following herbivory.
The data presented here covers plant individual and tissue-specific metabolite data as well as plant individual and tissue-specific biomass.
For detailed information how the data was acquired, please see the associated manuscript in Ecology and Evolution.
Description of the Data and file structure
General information: n/a denotes not available information, e.g., a metabolite that was not detected in a particular sample, or an empty cell in a remarks column for there were no remarks to be made during measurement
Dataset: Constitutive Metabolome_Meta-Data_PPI.csv
Column name Description
Composition Realized plant species community; Ger - Geranium pratense; Leu - Leucanthemum vulgare; Ran - Ranunculus acris
Sample.species Plant species name
Tissue Sampled plant tissue
Pot_Nr Running microcosm number
Diversity Plant species richness of the microcosm
Pos_1 Plant species position in any given microcosm
Pos_2 Plant species position in any given microcosm
Pos_3 Plant species position in any given microcosm
Pos_4 Plant species position in any given microcosm
Pos_5 Plant species position in any given microcosm
Pos_6 Plant species position in any given microcosm
Pos_7 Plant species position in any given microcosm
Pos_8 Plant species position in any given microcosm
Pos_9 Plant species position in any given microcosm
Pos_10 Plant species position in any given microcosm
Pos_11 Plant species position in any given microcosm
Pos_12 Plant species position in any given microcosm
Ger Number of planted Geranium individuals in any given microcosm
Leu Number of planted Leucanthemum individuals in any given microcosm
Ran Number of planted Ranunculus individuals in any given microcosm
sample.date Sample date
DWmg Dry weight in mg
Remarks Remarks
Dataset: Constitutive Metabolome_Meta-Data_PSI.csv
Column name Description
Composition Realized plant species community; Ger - Geranium pratense; Leu - Leucanthemum vulgare; Ran - Ranunculus acris
Sample.species Plant species name
Tissue Sampled plant tissue
Pot_Nr Running microcosm number
Diversity Plant species richness of the microcosm
Plot_ID Plot number of the Trait-Based Experiment used for sampling the live soil inoculum
sample.date Sample date
DWmg Dry weight in mg
Remarks Remarks
Dataset: Herbivore-induced Metabolome_Meta-Data_PPI.csv
Column name Description
Composition Realized plant species community; Ger - Geranium pratense; Leu - Leucanthemum vulgare; Ran - Ranunculus acris
Sample.species Plant species name
Pot_Nr Running microcosm number
Treatment Indicator if the sampled plant was a control plant or a herbivore-induced plant
Diversity Plant species richness of the microcosm
Pos_1 Plant species position in any given microcosm
Pos_2 Plant species position in any given microcosm
Pos_3 Plant species position in any given microcosm
Pos_4 Plant species position in any given microcosm
Pos_5 Plant species position in any given microcosm
Pos_6 Plant species position in any given microcosm
Pos_7 Plant species position in any given microcosm
Pos_8 Plant species position in any given microcosm
Pos_9 Plant species position in any given microcosm
Pos_10 Plant species position in any given microcosm
Pos_11 Plant species position in any given microcosm
Pos_12 Plant species position in any given microcosm
Ger Number of planted Geranium individuals in any given microcosm
Leu Number of planted Leucanthemum individuals in any given microcosm
Ran Number of planted Ranunculus individuals in any given microcosm
sample.date Sample date
DWmg Dry weight in mg
Remarks Remarks
Dataset: Herbivore-induced Metabolome_Meta-Data_PSI.csv
Column name Description
Composition Realized plant species community; Ger - Geranium pratense; Leu - Leucanthemum vulgare; Ran - Ranunculus acris
Sample.species Plant species name
Pot_Nr Running microcosm number
Treatment Indicator if the sampled plant was a control plant or a herbivore-induced plant
Diversity Plant species richness of the microcosm
Plot_ID Plot number of the Trait-Based Experiment used for sampling the live soil inoculum
sample.date Sample date
DWmg Dry weight in mg
Remarks Remarks
Datasets: Constitutive Metabolome species_PPI files (e.g., Constitutive Metabolome_Ger_pra_PPI_leaves.csv)
Column name Description
Sample.species Plant species name
Composition Realized plant species community; Ger - Geranium pratense; Leu - Leucanthemum vulgare; Ran - Ranunculus acris
Tissue Sampled plant tissue
Pot_Nr Running microcosm number
Diversity Plant species richness of the microcosm
sample.date Sample date
DWmg Dry weight in mg
richness Number of extracted and measured metabolites
shannon Shannon diversity of secondary metabolites
simpson Simpson diversity of secondary metabolites
ENS.shannon Shannon diversity of secondary metabolites expressed as effective number
ENS.simpson Simpson diversity of secondary metabolites expressed as effective number
The remaining columns Metabolite concentration (Intensity per mg dry weight); Column name first contains mass-to-charge ratio then retention time
Datasets: Constitutive Metabolome species_PSI files (e.g., Constitutive Metabolome_Ger_pra_PSI_leaves.csv)
Column name Description
Sample.species Plant species name
Composition Realized plant species community; Ger - Geranium pratense; Leu - Leucanthemum vulgare; Ran - Ranunculus acris
Tissue Sampled plant tissue
Pot_Nr Running microcosm number
Diversity Plant species richness of the microcosm
Plot_ID Plot number of the Trait-Based Experiment used for sampling the live soil inoculum
sample.date Sample date
DWmg Dry weight in mg
richness Number of extracted and measured metabolites
shannon Shannon diversity of secondary metabolites
simpson Simpson diversity of secondary metabolites
ENS.shannon Shannon diversity of secondary metabolites expressed as effective number
ENS.simpson Simpson diversity of secondary metabolites expressed as effective number
The remaining columns Metabolite concentration (Intensity per mg dry weight); Column name first contains mass-to-charge ratio then retention time
Datasets: Herbivore-induced Metabolome species_PPI files (e.g., Herbivore-induced Metabolome_Ger_pra_PPI.csv)
Column name Description
Sample.species Plant species name
Composition Realized plant species community; Ger - Geranium pratense; Leu - Leucanthemum vulgare; Ran - Ranunculus acris
Pot_Nr Running microcosm number
Treatment Indicator if the sampled plant was a control plant or a herbivore-induced plant
Diversity Plant species richness of the microcosm
sample.date Sample date
DWmg Dry weight in mg
richness Number of extracted and measured metabolites
shannon Shannon diversity of secondary metabolites
simpson Simpson diversity of secondary metabolites
ENS.shannon Shannon diversity of secondary metabolites expressed as effective number
ENS.simpson Simpson diversity of secondary metabolites expressed as effective number
The remaining columns Metabolite concentration (Intensity per mg dry weight); Column name first contains mass-to-charge ratio then retention time
Datasets: Herbivore-induced Metabolome species_PSI files (e.g., Herbivore-induced Metabolome_Ger_pra_PSI.csv)
Column name Description
Sample.species Plant species name
Composition Realized plant species community; Ger - Geranium pratense; Leu - Leucanthemum vulgare; Ran - Ranunculus acris
Pot_Nr Running microcosm number
Treatment Indicator if the sampled plant was a control plant or a herbivore-induced plant
Diversity Plant species richness of the microcosm
Plot_ID Plot number of the Trait-Based Experiment used for sampling the live soil inoculum
sample.date Sample date
DWmg Dry weight in mg
richness Number of extracted and measured metabolites
shannon Shannon diversity of secondary metabolites
simpson Simpson diversity of secondary metabolites
ENS.shannon Shannon diversity of secondary metabolites expressed as effective number
ENS.simpson Simpson diversity of secondary metabolites expressed as effective number
The remaining columns Metabolite concentration (Intensity per mg dry weight); Column name first contains mass-to-charge ratio then retention time