Data from: The origin of the odorant receptor gene family in insects

Brand, Philipp, University of California, Davis

Robertson, Hugh M., University of Illinois at Urbana Champaign

Lin, Wei, University of California, Davis

Pothula, Ratnasri, University of Tennessee at Knoxville

Klingeman, William E., University of Tennessee at Knoxville

Jurat-Fuentes, Juan Luis, University of Tennessee at Knoxville

Johnson, Brian R., University of California, Davis

Published Jul 26, 2019 on Dryad. https://doi.org/10.5061/dryad.p2t8170

Cite this dataset

Brand, Philipp et al. (2019). Data from: The origin of the odorant receptor gene family in insects [Dataset]. Dryad. https://doi.org/10.5061/dryad.p2t8170

Abstract

The origin of the insect odorant receptor (OR) gene family has been hypothesized to have coincided with the evolution of terrestriality in insects. Missbach et al. (2014) suggested that ORs instead evolved with an ancestral OR co-receptor (Orco) after the origin of terrestriality and the OR/Orco system is an adaptation to winged flight in insects. We investigated genomes of the Collembola, Diplura, Archaeognatha, Zygentoma, Odonata, and Ephemeroptera, and find ORs present in all insect genomes but absent from lineages predating the evolution of insects. Orco is absent only in the ancestrally wingless insect lineage Archaeognatha. Our new genome sequence of the zygentoman firebrat Thermobia domestica reveals a full OR/Orco system. We conclude that ORs evolved before winged flight, perhaps as an adaptation to terrestriality, representing a key evolutionary novelty in the ancestor of all insects, and hence a molecular synapomorphy for the Class Insecta.

Usage notes

Thermobia domestica genome assembly v 1.0

The Thermobia domestica genome assembly is based on a single individual from a laboratory colony. Briefly, we sequenced the DNA extracted from a single T. domestica individual for assembly with the DISCOVAR v1 pipeline (Weisenfeld et al., 2014). Quality assessment of the resulting assembly was based on standard N statistics, k-mer distribution analysis, and the BUSCO v2 pipeline (Simão et al., 2015) as previously described (Brand et al., 2017).

Tdom_v1.0.tgz

OR sequences of annotated species

FASTA format proteins for the newly described ORs of Ladona fulva, E. danica, T. domestica, M. hrabei. Suffixes, which are not part of the gene/protein name but indicate features of the gene model, are C – C-terminus missing, F – assembly was repaired, I – internal regions missing, J – gene model spans scaffolds, P – pseudogene.

OR_sequences.fa

OR alignment used for gene tree reconstruction (Figure 2., Figure S2)

CLUSTALX v2 alignment of all newly described ORs together with the 3 T. domestica ORs described by Missbach et al. 2014 and several neopteran Orcos.

OR_alignment.fa

Funding

National Science Foundation, Award: IOS-1456678,