Seven polymorphic microsatellite loci were scored in 292 specimens of Ae. japonicus collected in Belgium and Germany to investigate the population genetic structure of this invasive. The objectives of the study were to determine (i) if the mosquito specimens collected along the Belgian border were introduced from the nearby existing West German population, and (ii) if the population at Natoye (Belgium) resulted from a new introduction event and/or from a few undetected specimens that escaped elimination.

Aedes japonicus specimens were collected in the framework of different successive projects undertaken to monitor the introduction and establishment of exotic mosquito species in Belgium and Germany. DNA was extracted from legs, abdomens or eggs using either the NucleoSpin® Tissue DNA extraction kit (Macherey-Nagel) or the QIAamp DNA Micro kit (Qiagen). Specimens were genotyped for seven microsatellite loci developed for Ae. japonicus, using the two multiplexes as presented in Widdel et al. 2005 (sized on a 3130XL Genetic Analyzer, using GeneScanTM 500 LIZ as size standard). Microsatellite length variation visualization and determination was performed using Geneious® R11.

GENERAL INFORMATION
1. Title of Dataset: 
MICROSATELLITE GENOTYPES OF AEDES JAPONICUS COLLECTED IN BELGIUM AND GERMANY

2. Author Information

    A. Principal Investigator Contact Information

        Name: NATHALIE SMITZ
        Institution: ROYAL MUSEUM FOR CENTRAL AFRICA
        Address: LEUVENSESTEENWEG 17 3080 TERVUREN BELGIUM
        Email: NATHALIE.SMITZ@AFRICAMUSEUM.BE

    B. Associate or Co-investigator Contact Information

        Name: WIM VAN BORTEL
        Institution: OUTBREAK RESEARCH TEAM, INSTITUTE OF TROPICAL MEDICINE
        Address: NATIONALESTRAAT 155 2000 ANTWERP BELGIUM
        Email: WVANBORTEL@ITG.BE

    C. Alternate Contact Information

        Name: DEBLAUWE ISRA
        Institution: OUTBREAK RESEARCH TEAM, INSTITUTE OF TROPICAL MEDICINE
        Address: NATIONALESTRAAT 155 2000 ANTWERP BELGIUM
        Email: IDEBLAUWE@ITG.BE

3. Date of data collection (single date, range, approximate date): 

AEDES JAPONICUS SPECIMENS WERE COLLECTED FROM 2012 UNTIL 2019, DURING EXOTIC MOSQUITO MONITORING PROGRAMS.
MICROSATELLITE GENOTYPES WERE GENERATED IN 2020.

4. Geographic location of data collection: 

BELGIUM AND GERMANY: EXACT LOCATION DETAILS ARE DISPLAYED IN THE EXCEL SHEET FOR EACH PROCESSED SPECIMEN.
LABPRATORY MANIPULATIONS WERE REALISED IN THE ROYAL MUSEUM FOR CENTRAL AFRICA.

5. Information about funding sources that supported the collection of the data: 

This work in Belgium is part of the MEMO project, funded by the Flemish, Walloon and Brussels regional governments and the Federal Public Service (FPS) Public Health, Food Chain Safety and Environment in the context of the National Environment and Health Action Plan (NEHAP) (Belgium). 
The Barcoding Facility for Organisms and Tissues of Policy Concern (BopCo - http://bopco.myspecies.info/) is financed by the Belgian Science Policy Office (Belspo) as Belgian federal in-kind contribution to the European Research Infrastructure Consortium “LifeWatch”. 
The Outbreak Research Team of the Institute of Tropical Medicine is funded by the Department of Economy, Science and Innovation of the Flemish government.

SHARING/ACCESS INFORMATION

1. Was data derived from another source? 

NO

2. Recommended citation for this dataset: 

PLEASE CITE THE PUBLICATION OF SMITZ ET AL., IN WHICH THE DRYAD DOI IS CITED FIRST.

DATA & FILE OVERVIEW

1. File List: 

Smitz et al. Aedes_japonicus_microsatellite_genotypes.xls: EXCEL SHEET WITH THE MICROSATELLITE GENOTYPES FOR EACH PROCESSED SPECIMEN, INCLUDING COLLECTION LOCATION AND YEAR.

2. Additional related data collected that was not included in the current data package: 

NAD4 FRAGMENT WAS ALSO SCORED IN 278 SPECIMENS AND DEPOSITED IN GENBANK WITH ACCESSION NUMBERS: MT462702 - MT462979.

3. Are there multiple versions of the dataset?

NO

METHODOLOGICAL INFORMATION
1. Description of methods used for collection/generation of data: 

Aedes japonicus specimens were collected in the framework of different successive projects undertaken to monitor the introduction and establishment of exotic mosquito species in Belgium and Germany.
DNA was extracted from legs, abdomens or eggs using either the NucleoSpin® Tissue DNA extraction kit (Macherey-Nagel) or the QIAamp DNA Micro kit (Qiagen).

2. Methods for processing the data: 

Specimens were genotyped for seven microsatellite loci developed for Ae. japonicus, using the two multiplexes presented in Widdel et al. 2005.

3. Instrument- or software-specific information needed to interpret the data: 

Microsatellite length variation visualization and determination was performed using Geneious® R11.

4. People involved with sample collection, processing, analysis and/or submission: 

Additional collaborators are: Katrien De Wolf, Helge Kampen, Francis Schaffner, Jacobus De Witte, Anna Schneider, Ingrid Verlé, Adwine Vanslembrouck, Wouter Dekoninck, Kenny Meganck, Sophie Gombeer, Ann Vanderheyden, Marc De Meyer, Thierry Backeljau, Doreen Werner, Ruth Müller

DATA-SPECIFIC INFORMATION FOR: Smitz et al. Aedes_japonicus_microsatellite_genotypes.xls

1. Number of variables: 12

2. Number of rows: 586 (diploid organism-> two rows per individual)

3. Variable List: 

Column A: "Sample Code" - code attribuated to each specimen
Column B: "Country" - either Germany or Belgium, Europe
Column C: "State / Province" - State where the specimen was collected in Germany, and province where the specimen was collected in Belgium
Column D: "Locality" - name of the city where the specimen was collected
Column E: "Collection year" - year of collection (2012 until 2019)
Column F - L: "Microsatellite genotypes" - size of each microsatellite loci (N = 7), sized on a 3130XL Genetic Analyzer, using GeneScanTM 500 LIZ as size standard

4. Missing data code: -9