Data from: Amyloid β induces hormetic-like effects through major stress pathways in a C. elegans model of Alzheimer’s disease
Data files
Dec 06, 2024 version files 43.86 KB
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DataSet_Licthy_et_al_2024.xlsx
36.10 KB
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README.md
7.77 KB
Abstract
Amyloid β (Aβ) is a peptide known for its characteristic aggregates in Alzheimer’s Disease and its ability to induce a wide range of detrimental effects in various model systems. However, Aβ has also been shown to induce some beneficial effects, such as antimicrobial properties against pathogens. In this work, we explore the influence of Aβ in stress resistance in a C. elegans model of Alzheimer’s Disease. We found that C. elegans that express human Aβ exhibit increased resistance to heat and anoxia, but not to oxidative stress. This beneficial effect of Aβ was driven from Aβ in neurons, where the level of induction of Aβ expression correlated with stress resistance levels. Transcriptomic analysis revealed that this selective stress resistance was mediated by the Heat Shock Protein (HSPs) family of genes. Furthermore, neuropeptide signaling was necessary for Aβ to induce stress resistance, suggesting neuroendocrine signaling plays a major role in activating organismal stress response pathways. These results highlight the potential beneficial role of Aβ in cellular function, as well as its complex effects on cellular and organismal physiology that must be considered when using C. elegans as a model for Alzheimer’s Disease.
README: Data for Amyloid β Induces Hormetic-Like Effects Through Major Stress Pathways in a C. elegans Model of Alzheimer’s Disease
Data includes results from experiments using a C. elegans strain that expresses human amyloid-beta fluorescently labeled at substoichiometric ratios.
Contact Adriana San Miguel ([asanmig@ncsu.edu]) with any questions. This manuscript has been accepted for publication (12/2024):
Lichty, J.D.; Mane, H., Yarmey, V.R., San Miguel, A. Amyloid β Induces Hormetic-Like Effects Through Major Stress Pathways in a C. elegans Model of Alzheimer’s Disease. PLOS One (accepted).
Data files include all data presented in the above-referenced manuscript.
DataSet_Lichty_et_al_2024.xlsx - Tabs Figure 1B, Figure 1C, Figure 1D
variables | units | description |
---|---|---|
Date | date | Date experiment was performed |
Biological Replicate | categorical | Biological replicates are individually cultured and age synchronized populations |
Strain | categorical | Strain used in the experiment |
Technical Replicate | categorical | Subpopulations obtained from the biological replicate |
Alive | number | Animals that crawled spontaneously or upon prodding |
Dead | number | Animals identified as dead by lack of movement upon prodding |
Missing | number | Animals not identified during counting |
Strains:
N2 = WT
JKM2 = Aβ+
JKM3 = Aβ-
DataSet_Lichty_et_al_2024.xlsx - Tab Figure 2B, 2C
variables | units | description |
---|---|---|
Date | date | Date experiment was performed |
Biological Replicate | categorical | Biological replicates are individually cultured and age synchronized populations |
Strain | categorical | Strain used in the experiment |
Time Spent at 25C | number | Duration of exposure to 25C (hours) prior to stress assay |
Technical Replicate | categorical | Subpopulations obtained from the biological replicate |
Alive | number | Animals that crawled spontaneously or upon prodding |
Dead | number | Animals identified as dead by lack of movement upon prodding |
Missing | number | Animals not identified during counting |
Strains:
N2 = WT
CL2355 = nAβ
CL4176 = mAβ
DataSet_Lichty_et_al_2024.xlsx - Tab Figure 3A
variables | units | description |
---|---|---|
Date | date | Date experiment was performed |
Biological Replicate | categorical | Biological replicates are individually cultured and age synchronized populations |
Strain | categorical | Strain used in the experiment |
Technical Replicate | categorical | Subpopulations obtained from the biological replicate |
Alive | number | Animals that crawled spontaneously or upon prodding |
Dead | number | Animals identified as dead by lack of movement upon prodding |
Missing | number | Animals not identified during counting |
Strains:
N2 = WT
OH438 = nGFP
DataSet_Lichty_et_al_2024.xlsx - Tab Figure 3B
variables | units | description |
---|---|---|
Date | date | Date experiment was performed |
Biological Replicate | categorical | Biological replicates are individually cultured and age synchronized populations |
Strain | categorical | Strain used in the experiment |
NAC Concentration | number | Concentration of N-acetyl cysteine used in the experiment (mM) |
Technical Replicate | categorical | Subpopulations obtained from the biological replicate |
Alive | number | Animals that crawled spontaneously or upon prodding |
Dead | number | Animals identified as dead by lack of movement upon prodding |
Missing | number | Animals not identified during counting |
Strains:
N2 = WT
JKM2 = Aβ+
JKM3 = Aβ-
DataSet_Lichty_et_al_2024.xlsx - Tab Figure 4A, 4B, 4C
variables | units | description |
---|---|---|
Gene | categorical | Gene assessed in expression assay |
Strain | categorical | Strain used in the experiment |
Count | number | Transcripts obtained for the gene using Nanostring gene expression assay |
Strains:
N2 = WT
JKM2 = Aβ+
JKM3 = Aβ-
DataSet_Lichty_et_al_2024.xlsx - Tab Figure 4D
variables | units | description |
---|---|---|
Date | date | Date experiment was performed |
Biological Replicate | categorical | Biological replicates are individually cultured and age synchronized populations |
Strain | categorical | Strain used in the experiment |
RNAi Treatment | categorical | Gene targeted by RNAi or none (Control) |
Technical Replicate | categorical | Subpopulations obtained from the biological replicate |
Alive | number | Animals that crawled spontaneously or upon prodding |
Dead | number | Animals identified as dead by lack of movement upon prodding |
Missing | number | Animals not identified during counting |
Strains:
NL2099 = rrf-3(-)
ASM35 = Aβ+;rrf-3(-) This strain is a cross of JKM2 and NL2099 generated in this work.