Supplemental Movie 1: Adapted rotating-disc rheometry measurement. Left panel is a recording of the rheometry measurement for an untreated 5 d S. gordonii biofilm. Right panel indicates the corresponding torque – angular velocity data collection. Individual frames from the time-lapse depicting separate biofilm detachment events are displayed in Fig 2. Timestamp is indicated in the top left-hand corner (min : s). Playback rate is at 15 fps.

Supplemental Movie 2: Transformed data collection. Left panel is the same recording depicted in movie S1. Right panel depicts the corresponding torque – angular velocity data that has been linearized and transformed to emphasis the changes in torque. Individual frames from the time lapse depicting separate biofilm detachment events are displayed in Fig 2. Timestamp is indicated in the top left-hand corner (min : s). Playback rate is at 15 fps.