Data from: High-density cultivation of microalgae continuously fed with unfiltered water from a recirculating aquaculture system
Egloff, Sophia, University of Zurich
Tschudi, Fridolin, University of Zurich
Schmautz, Zala, University of Zurich
Refardt, Dominik, University of Zurich
Published Jul 11, 2019 on Dryad.
Cite this dataset
Egloff, Sophia; Tschudi, Fridolin; Schmautz, Zala; Refardt, Dominik (2019). Data from: High-density cultivation of microalgae continuously fed with unfiltered water from a recirculating aquaculture system [Dataset]. Dryad. https://doi.org/10.5061/dryad.p9m57n7
Water from recirculating aquaculture systems (RAS) has been shown to be a suitable growth medium for microalgae and their cultivation can, therefore, be used to reduce RAS emissions. However, while efficient wastewater treatment is possible, the nutrient content of RAS water limits attainable microalgae biomass densities to 1–2 g l−1 at best, which requires frequent harvesting of microalgae. We have taken advantage of the constant evaporation of water from an open thin-layer photobioreactor (200 l volume, 18 m2 illuminated surface, artificial supply of CO2) to continuously add water from RAS to a microalgae culture and thereby provide nutrients for continued growth while evaporating all water. To test for a possible inhibitory effect of RAS water on microalgae growth, components of mineral medium were omitted stepwise in subsequent cultivations and replaced by RAS water as the only source of nutrients. This approach showed that microalgae can be grown successfully for up to three weeks in RAS water without additional nutrients and that high (20 g l−1) biomass densities can be attained. While growth in wastewater did not reach productivities measured in mineral medium, analysis of growth data suggested that this reduction was not due to an inhibitory effect of the RAS water but due to an insufficient supply rate of nutrients, even though RAS water contained up to 158 mg l−1 NO3-N. It is, therefore, concluded that this method can be used to fully treat the wastewater discharge of a RAS. Furthermore, because both water evaporation from and microalgae growth in the photobioreactor correlated positively with each other due to their shared dependency on solar radiation, supply of nutrients continuously adjusts to changes in demand. It is estimated that the area of a photobioreactor required to treat all emissions of a RAS requires approximately 6.5 times the area of the latter.
Raw data for cultivation no. 1
Raw data of cultivation no. 2
Raw data for cultivation no. 3
Raw data for cultivation no. 4
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Raw data of cultivation no. 6
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Raw data for cultivation no. 8
Raw data for cultivation no. 9
Local sunrise and sunset data
R script to make all raw data ready for analysis
The script requires the raw data in csv format and data on sunrise/sunset times. The script truncates the data correctly, removes false values, renames variables and saves all data as all.Data.RData
R script to carry out statistical analyses and produce figures
This r script opens the file all.Data.RData (produced via the script 1_prepare_data.R) and conducts all statistical analyses and figures.