https://doi.org/10.5061/dryad.pc866t1wd

Hugues Beaufrère, University of California - Davis
Lisa Pacumio,University of California - Davis
Leonardo Susta, University of Guelph
Danielle Tarbert, University of Tennessee
Mélanie Ammersbach, University of California - Davis
Kevin Keel, University of California - Davis

Abstract

Forty-eight adult bearded dragons were used in this study to explore the associations between plasma metabolites, plasma biochemsitry, and plasma liporproteins and hepatic fat accumulation.
Plasma was submitted for untargeted primary metabolomics using gas chromatography time-of-flight mass spectrometry, a biochemistry panel, and a lipoprotein panel determined by high-resolution polyacrylamide gel electrophoresis. Hepatic lipid content was quantified by liver attenuation measurements from computed tomography images and digital image analysis of standardized histologic sections of the liver. Fibrosis was quantified by digital image analysis on Masson’s trichrome stained histologic sections. Severity was determined from pathologic review of liver sections according to a standardized grading system.

Usage notes

• Bearded dragons with other liver disease (N in other.liver.disease column) were excluded from analysis.
• Biochemistry was performed at the UC Davis - School of Veterinary Medicine - Veterinary Medicine Teaching Hospital - Veterinary Diagnostic Laboratory.
• Insulin measurement was performed at the University of Michigan - College of Veterinary Medicine - Veterinary Diagnostic Laboratory
• Metabolomics was performed at the UC Davis - West Coast Metabolomics Center
• Lipoprotein profiling was performed at the UC Davis - Comparative Clinical Lipidology Laboratory
• Histologic grade and severity score of hepatic liver accumulation were obtained according to a published grading system in this species: https://pubmed.ncbi.nlm.nih.gov/36250570/

Datasets included:

1. Dataset including 48 bearded dragons with histologic grade of hepatic fat accumulation, the presence of other liver disease, weight in grams, age in years, computed tomography liver attenuation in Hounsfield units, fat and fibrosis percentage determined by digital image analysis, and all measurands. The measurement units of analytes and metabolites are as follow: -Insulin: uIU/mL -Triglycerides, calcium, uric acid, cholesterol: mg/dL -Na, K, Cl: mmol/L -Bile acids: umol/L -AST, ALT, ALP, CK, LDH, GLDH: IU/mL -all others: peak intensity on mass spectrometry
2. Same dataset with lipoprotein panel, some having missing values (failed electrophoretic migration). Lipoproteins are measured in either percentage (when suffix ".perc" is present) or in mg/dL of cholesterol concentration.

Abbreviations:

• HU: Hounsfield unit
• GLDH: glutamate dehydrogenase
• LDH: lactate dehydrogenase
• AST: aspartate aminotransferase
• ALP: alkaline phosphatase
• ALT: alanine transaminase
• CK: creatine kinase
• VLDL: very low density lipoprotein
• LDL: low density lipoprotein
• HDL: high density lipoprotein
• UDP-glucuronic acid: uridine diphosphate glucuronic acid