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Quantitative Multiplex Immunoprecipitation (QMI) analysis of protein interaction network rearrangements during homeostatic plasticity

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Apr 16, 2021 version files 1.02 GB

Abstract

Neurons maintain constant levels of excitability using homeostatic synaptic scaling, which adjusts the strength of all of a neuron’s postsynaptic inputs to compensate for large changes in overall activity. Here, we asked how prolonged changes in activity affect network-level protein interactions at the synapse. We assessed a glutamatergic synapse protein interaction network (PIN) composed of 380 binary associations among 21 protein members to identify protein complexes altered by activity manipulation in vitro or sensory deprivation in vivo. In cultured cortical neurons, we observed widespread bidirectional PIN alterations during up- and downscaling that reflected rapid rearrangements of glutamate receptor co-associations via synaptic scaffold remodeling. Sensory deprivation of the barrel cortex caused unique PIN rearrangements, including changes in co-associations between the glutamate receptor mGluR5 and the kinase Fyn, consistent with emerging models of experience dependent plasticity involving multiple types of homeostatic responses. Mice lacking Homer1 or Shank3B did not undergo normal PIN rearrangements, suggesting that these Autism Spectrum Disorder (ASD)-linked proteins serve as structural hubs for synaptic homeostasis. This dataset contains the raw XML and PBX (bioplex protocol) files necessary for performing adaptive non-parametric analysis with an emprical alpha cutoff (ANC) to identify high-confidence IP_Probe pairs, here called "PiSCES" for proteins in shared complexes detected by surface epitopes, that are signifianctly differently associated between two conditions.