This dataset submission consists of a DNA matrix and two tree files. The phylogeny file of UCE loci for 39 taxa was required to construct a Maximum Likelihood tree. The tree file was needed to examine the topology and the topology was used to examine the limits of two taxonomically challenging genera within the tribe Pepsini, Cryptocheilus and Heterodontonyx. The time-calibrated tree was built with MCMCTree program to estimate the divergence pattern and timing of the group.