Bloodmeal metabarcoding of the argasid tick (Ornithodoros turicata Dugès) reveals extensive vector-host associations

Balasubramanian, Sujata1 ; Busselman, Rachel1 ; Fernandez-Santos, Nadia1 ; Grunwald, Andrew2 ; Wolff, Nicholas1 ; Hathaway, Nicholas3 ; Hillhouse, Andrew1 ; Bailey, Jeffrey4 ; Teel, Pete1; Ferreira, Francisco1 ; Hamer, Sarah1 ; Hamer, Gabriel1

Published Jan 31, 2024 on Dryad. https://doi.org/10.5061/dryad.pg4f4qrx0

Data files

Jan 31, 2024 version files 45.26 GB

GCSNAIndex-1_S1_L001_R1_001.fastq.gz

170.70 MB
GCSNAIndex-1_S1_L001_R2_001.fastq.gz

196.03 MB
GCSNAIndex-1_S1_L002_R1_001.fastq.gz

165 MB
GCSNAIndex-1_S1_L002_R2_001.fastq.gz

191.42 MB
GCSNAIndex-1_S1_L003_R1_001.fastq.gz

170.11 MB
GCSNAIndex-1_S1_L003_R2_001.fastq.gz

196.97 MB
GCSNAIndex-1_S1_L004_R1_001.fastq.gz

168.14 MB
GCSNAIndex-1_S1_L004_R2_001.fastq.gz

195.07 MB
GCSNAIndex-2_S2_L001_R1_001.fastq.gz

164.06 MB
GCSNAIndex-2_S2_L001_R2_001.fastq.gz

191.45 MB
GCSNAIndex-2_S2_L002_R1_001.fastq.gz

158.61 MB
GCSNAIndex-2_S2_L002_R2_001.fastq.gz

186.72 MB
GCSNAIndex-2_S2_L003_R1_001.fastq.gz

164.06 MB
GCSNAIndex-2_S2_L003_R2_001.fastq.gz

192.64 MB
GCSNAIndex-2_S2_L004_R1_001.fastq.gz

161.94 MB
GCSNAIndex-2_S2_L004_R2_001.fastq.gz

190.64 MB
GCSNAIndex-3_S3_L001_R1_001.fastq.gz

159.30 MB
GCSNAIndex-3_S3_L001_R2_001.fastq.gz

186.88 MB
GCSNAIndex-3_S3_L002_R1_001.fastq.gz

153.85 MB
GCSNAIndex-3_S3_L002_R2_001.fastq.gz

182.52 MB
GCSNAIndex-3_S3_L003_R1_001.fastq.gz

159.17 MB
GCSNAIndex-3_S3_L003_R2_001.fastq.gz

188.40 MB
GCSNAIndex-3_S3_L004_R1_001.fastq.gz

156.80 MB
GCSNAIndex-3_S3_L004_R2_001.fastq.gz

185.77 MB
GCSNAIndex-4_S4_L001_R1_001.fastq.gz

176.42 MB
GCSNAIndex-4_S4_L001_R2_001.fastq.gz

202.23 MB
GCSNAIndex-4_S4_L002_R1_001.fastq.gz

170.66 MB
GCSNAIndex-4_S4_L002_R2_001.fastq.gz

197.54 MB
GCSNAIndex-4_S4_L003_R1_001.fastq.gz

176.12 MB
GCSNAIndex-4_S4_L003_R2_001.fastq.gz

203.33 MB
GCSNAIndex-4_S4_L004_R1_001.fastq.gz

173.82 MB
GCSNAIndex-4_S4_L004_R2_001.fastq.gz

201.16 MB
GCSNAIndex-5_S5_L001_R1_001.fastq.gz

161.27 MB
GCSNAIndex-5_S5_L001_R2_001.fastq.gz

186.72 MB
GCSNAIndex-5_S5_L002_R1_001.fastq.gz

155.95 MB
GCSNAIndex-5_S5_L002_R2_001.fastq.gz

182.49 MB
GCSNAIndex-5_S5_L003_R1_001.fastq.gz

161.08 MB
GCSNAIndex-5_S5_L003_R2_001.fastq.gz

187.98 MB
GCSNAIndex-5_S5_L004_R1_001.fastq.gz

158.57 MB
GCSNAIndex-5_S5_L004_R2_001.fastq.gz

185.54 MB
GCSNAIndex-6_S6_L001_R1_001.fastq.gz

146.84 MB
GCSNAIndex-6_S6_L001_R2_001.fastq.gz

170.37 MB
GCSNAIndex-6_S6_L002_R1_001.fastq.gz

141.96 MB
GCSNAIndex-6_S6_L002_R2_001.fastq.gz

166.38 MB
GCSNAIndex-6_S6_L003_R1_001.fastq.gz

146.95 MB
GCSNAIndex-6_S6_L003_R2_001.fastq.gz

171.91 MB
GCSNAIndex-6_S6_L004_R1_001.fastq.gz

144.49 MB
GCSNAIndex-6_S6_L004_R2_001.fastq.gz

169.17 MB
GCSNAIndex-7_S7_L001_R1_001.fastq.gz

149.47 MB
GCSNAIndex-7_S7_L001_R2_001.fastq.gz

170.76 MB
GCSNAIndex-7_S7_L002_R1_001.fastq.gz

144.72 MB
GCSNAIndex-7_S7_L002_R2_001.fastq.gz

166.80 MB
GCSNAIndex-7_S7_L003_R1_001.fastq.gz

149.76 MB
GCSNAIndex-7_S7_L003_R2_001.fastq.gz

172.28 MB
GCSNAIndex-7_S7_L004_R1_001.fastq.gz

147.05 MB
GCSNAIndex-7_S7_L004_R2_001.fastq.gz

169.39 MB
LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz

1.28 GB
LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz

1.53 GB
LA-ST_BM_Pool_02_S7_L001_R1_001.fastq.gz

1.04 GB
LA-ST_BM_Pool_02_S7_L001_R2_001.fastq.gz

1.21 GB
LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz

1.47 GB
LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz

1.83 GB
LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz

2.10 GB
LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz

2.45 GB
LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz

1.98 GB
LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz

2.37 GB
LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz

1.33 GB
LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz

1.61 GB
LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz

2.43 GB
LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz

2.90 GB
LA-ST_BM_Pool_08_S13_L001_R1_001.fastq.gz

1.42 GB
LA-ST_BM_Pool_08_S13_L001_R2_001.fastq.gz

1.64 GB
LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz

1.28 GB
LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz

1.50 GB
LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz

1.87 GB
LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz

2.38 GB
README.md

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Abstract

Molecular methods to understand host feeding patterns of arthropod vectors are critical to assess exposure risk to vector-borne disease and unveil complex ecological interactions. We build on our prior work discovering the utility of PCR-Sanger sequencing bloodmeal analysis that work well for soft ticks (Acari: Argasidae), unlike for hard ticks (Acari: Ixodidae), thanks to their unique physiology that retains prior bloodmeals for years. Here, we apply bloodmeal metabarcoding using amplicon deep sequencing to identify multiple host species in individual Ornithodoros turicata soft ticks collected from two natural areas in Texas, United States. Of 788 collected O. turicata, 394 were evaluated for bloodmeal source via metabarcoding, revealing 27 different vertebrate hosts (17 mammals, 5 birds, 1 reptile, and 4 amphibians) fed upon by 274 soft ticks. Information on multiple hosts was derived from 167 individual O. turicata (61%). Metabarcoding revealed mixed vertebrate bloodmeals in O. turicata while same specimens yielded only one vertebrate species using Sanger sequencing. These data reveal wide host range of O. turicata and demonstrate the value of bloodmeal metabarcoding for understanding the ecology for known and potential tick-borne pathogens circulating among humans, domestic animals and wildlife such as relapsing fever caused by Borrelia turicatae. Our results also document evidence of prior feeding on wild pig from an off-host soft tick for the first time in North America; a critical observation in the context of enzootic transmission of African swine fever virus if it were introduced to the US. This research enhances our understanding of vector-host associations and offers a promising perspective for biodiversity monitoring and disease control strategies.

README: Bloodmeal metabarcoding of the argasid tick (Ornithodoros turicata Dugès) reveals extensive vector-host associations.

https://doi.org/10.5061/dryad.pg4f4qrx0

Soft ticks (Ornithodoros turicata) were collected using sticky traps from two locations in Texas - Government Canyon State Natural Area and Laguna Atascosa National Wildlife Refuge. Soft ticks are hematorphagous and vector various viral and bacterial disease. The O. turicata sof ticks collected were identfied to life stage and species and individually analysed for the vertebrate bloodmeal to establish a host network for these soft ticks.

Description of data:

This data deposit contains raw data from next generation Illumina sequencing of metabarcoding PCR looking for blood meal hosts consumed by Ornithodoros turicata collected from two locations in Texas, USA. Primers used to amplify bloodmeals were 12s rRNA primers - forward 12 S rRNA Primer 5'CAAACTGGGATTAGATACC3' Reverse 12 S rRNA Primer 5'AGAACAGGCTCCTCTAG3', from Humair et al., 2007 (Humair, P.-F. O., Douet, V. R., Cadenas, F. M. N., & Schouls, L. M. (2007). Molecular Identiﬁcation of Bloodmeal Source in Ixodes ricinus Ticks Using 12S rDNA As a Genetic Marker. Journal of Medical Entomology, 44(5), 12). Individual amplicons are tagged with 10 digit bacodes listed below and are from Shokralla et al., 2014 (Shokralla, S., Gibson, J. F., Nikbakht, H., Janzen, D. H., Hallwachs, W., & Hajibabaei, M. (2014). Next_generation DNA barcoding: Using next_generation sequencing to enhance and accelerate DNA barcode capture from single specimens. Molecular Ecology Resources, 14(5), 892–901. https://doi.org/10.1111/1755-0998.12236.) The list of samples is presented in the table below and the file SequenceFilesandBarcodes.xlsx. Sequencing files are in fastq format. (Cock PJ, Fields CJ, Goto N, Heuer ML, Rice PM. The Sanger FASTQ file format for sequences with quality scores, and the Solexa/Illumina FASTQ variants. Nucleic Acids Res. 2010 Apr;38(6):1767-71. doi: 10.1093/nar/gkp1137. Epub 2009 Dec 16. PMID: 20015970; PMCID: PMC2847217) from Illumina Novaseq 6000. In order to derive data for indivdual ticks, sequencing read files can be unzipped and merged from R1 and R2 files for viewing or for further bioinformatics analyses following the file structure described below.

Analysis and results are published in the above mentioned paper "Bloodmeal metabarcoding of the argasid tick (Ornithodoros turicata Dugès) reveals extensive vector-host associations."

File structure:

Data from the location Laguna Atascosa National Wildlife Refuge LANWR (see manuscript) data is located in 20 files. Files have R1 and R2 fastq files which are needed for samples listed under that pool number in the table

Data from the location Government Canyon State Natural area GCSNA (see manuscript) is located in 56 files. The samples corresponding to each file are listed in the table below, 4 files each with R1 and R2 files. Data from all four files are needed for samples listed under that GCSNA index in the table

This table below is also submitted as a supplemental file SequenceFilesandBarcodes.xlsx

SampleName	SequencingData File Name R1	SequencingData File Name R2	Barcode Tag for Replicate 1	Barcode Tag for Replicate 2	Sitename/Number	Study Location
LA-1A	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	ACGAGTGCGT	ACGCTCGACA	LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-1D	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-1H	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	TCACGTACTA		LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-1I	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	TGTACTACTC		LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-1J	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	ACGACTACAG	CGTAGACTAG	LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-1K	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	TACTCTCGTG		LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-1L	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	TCGTCGCTCG		LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-1M	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	ACATACGCGT	ACGCGAGTAT	LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-1N	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-1O	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	AGACTATACT	AGCGTCGTCT	LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-1P	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	AGTACGCTAT		LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-1Q	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	CACGCTACGT	CAGTAGACGT	LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-1R	LA-ST_BM_Pool_01_S6_L001_R1_001.fastq.gz	LA-ST_BM_Pool_01_S6_L001_R2_001.fastq.gz	CGACGTGACT	TACACACACT	LA-1	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-2E	LA-ST_BM_Pool_02_S7_L001_R1_001.fastq.gz	LA-ST_BM_Pool_02_S7_L001_R2_001.fastq.gz	ACGCTCGACA		LA-2	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-2P	LA-ST_BM_Pool_02_S7_L001_R1_001.fastq.gz	LA-ST_BM_Pool_02_S7_L001_R2_001.fastq.gz	CGAGAGATAC	ATACGACGTA	LA-2	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-2X	LA-ST_BM_Pool_02_S7_L001_R1_001.fastq.gz	LA-ST_BM_Pool_02_S7_L001_R2_001.fastq.gz	TACGAGTATG	TACTCTCGTG	LA-2	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-2AA	LA-ST_BM_Pool_02_S7_L001_R1_001.fastq.gz	LA-ST_BM_Pool_02_S7_L001_R2_001.fastq.gz	TCGTCGCTCG		LA-2	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-2AD	LA-ST_BM_Pool_02_S7_L001_R1_001.fastq.gz	LA-ST_BM_Pool_02_S7_L001_R2_001.fastq.gz	ACATACGCGT		LA-2	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-2AM	LA-ST_BM_Pool_02_S7_L001_R1_001.fastq.gz	LA-ST_BM_Pool_02_S7_L001_R2_001.fastq.gz	AGTACGCTAT	ATAGAGTACT	LA-2	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-2AP	LA-ST_BM_Pool_02_S7_L001_R1_001.fastq.gz	LA-ST_BM_Pool_02_S7_L001_R2_001.fastq.gz	CGACGTGACT	TACACACACT	LA-2	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-2AQ	LA-ST_BM_Pool_02_S7_L001_R1_001.fastq.gz	LA-ST_BM_Pool_02_S7_L001_R2_001.fastq.gz	TACACGTGAT	TACAGATCGT	LA-2	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-2AY	LA-ST_BM_Pool_02_S7_L001_R1_001.fastq.gz	LA-ST_BM_Pool_02_S7_L001_R2_001.fastq.gz	TCTAGCGACT	TCTATACTAT	LA-2	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6A	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	ACGAGTGCGT	ACGCTCGACA	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6E	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	AGACGCACTC	AGCACTGTAG	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6M	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6Q	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	CATAGTAGTG		LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6V	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	TCACGTACTA	CGTCTAGTAC	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6AB	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	ACGACTACAG	CGTAGACTAG	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6AD	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	TACGAGTATG	TACTCTCGTG	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6AM	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	ACGCGAGTAT		LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6AN	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6AP	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	AGACTATACT	AGCGTCGTCT	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6AQ	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	AGTACGCTAT	ATAGAGTACT	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6AV	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	CACGCTACGT		LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6AW	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	CGACGTGACT	TACACACACT	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6AX	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	TACACGTGAT		LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6BB	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	TACGCTGTCT	TAGTGTAGAT	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6BO	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	TCTAGCGACT	TCTATACTAT	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6BX	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	ACTAGCAGTA	AGCTCACGTA	LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-6CA	LA-ST_BM_Pool_03_S8_L001_R1_001.fastq.gz	LA-ST_BM_Pool_03_S8_L001_R2_001.fastq.gz	AGTATACATA		LA-6	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7A	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	ACGAGTGCGT		LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7C	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	AGACGCACTC	AGCACTGTAG	LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7D	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7F	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	CATAGTAGTG		LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7G	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	CGAGAGATAC	ATACGACGTA	LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7H	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	TCACGTACTA		LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7I	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	TCTACGTAGC	TGTACTACTC	LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7J	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	ACGACTACAG	CGTAGACTAG	LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7L	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	TCGTCGCTCG		LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7M	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	ACGCGAGTAT		LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7N	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7P	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	AGTACGCTAT	ATAGAGTACT	LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7R	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	CGACGTGACT		LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7S	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	TACACGTGAT		LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7U	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	TCGATCACGT	TCGCACTAGT	LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7V	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	TCTAGCGACT	TCTATACTAT	LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7W	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	TGACGTATGT	TGTGAGTAGT	LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-7Y	LA-ST_BM_Pool_04_S9_L001_R1_001.fastq.gz	LA-ST_BM_Pool_04_S9_L001_R2_001.fastq.gz	ACTAGCAGTA	AGCTCACGTA	LA-7	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8B	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	ACGAGTGCGT	ACGCTCGACA	LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8D	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	AGACGCACTC	AGCACTGTAG	LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8E	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8M	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	CTCGCGTGTC	TCTCTATGCG	LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8N	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	TGATACGTCT	CATAGTAGTG	LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8BC	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	TCACGTACTA	CGTCTAGTAC	LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8BD	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	TGTACTACTC		LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8BK	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	ACGACTACAG	CGTAGACTAG	LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8BQ	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	TACGAGTATG	TACTCTCGTG	LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8BT	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	TCGTCGCTCG		LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8BX	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	ACGCGAGTAT		LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8CR	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	AGTACGCTAT		LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8DA	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	CGACGTGACT	TACACACACT	LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8DD	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	TACACGTGAT		LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8DW	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	TCGATCACGT		LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8DX	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	TCTAGCGACT		LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8DY	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	TGACGTATGT		LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8DZ	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	ACGCGATCGA		LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-8EB	LA-ST_BM_Pool_05_S10_L001_R1_001.fastq.gz	LA-ST_BM_Pool_05_S10_L001_R2_001.fastq.gz	AGTATACATA	AGTCGAGAGA	LA-8	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14A	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	ACGAGTGCGT	ACGCTCGACA	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14B	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	AGACGCACTC	AGCACTGTAG	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14C	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14D	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	CTCGCGTGTC		LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14F	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	CGAGAGATAC	ATACGACGTA	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14G	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	TCACGTACTA	CGTCTAGTAC	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14H	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	TCTACGTAGC	TGTACTACTC	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14I	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	ACGACTACAG	CGTAGACTAG	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14J	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	TACGAGTATG	TACTCTCGTG	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14K	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	TCGTCGCTCG		LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14L	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	ACATACGCGT	ACGCGAGTAT	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14M	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14N	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	AGACTATACT	AGCGTCGTCT	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14O	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	AGTACGCTAT	ATAGAGTACT	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14P	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	CACGCTACGT	CAGTAGACGT	LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-14Q	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	TACACACACT		LA-14	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-23C	LA-ST_BM_Pool_06_S11_L001_R1_001.fastq.gz	LA-ST_BM_Pool_06_S11_L001_R2_001.fastq.gz	TCTAGCGACT	TCTATACTAT	LA-23	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15A	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	ACGAGTGCGT	ACGCTCGACA	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15B	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	AGACGCACTC	AGCACTGTAG	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15C	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15D	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	CTCGCGTGTC	TCTCTATGCG	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15E	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	TGATACGTCT	CATAGTAGTG	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15G	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	TCACGTACTA	CGTCTAGTAC	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15H	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	TCTACGTAGC	TGTACTACTC	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15I	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	ACGACTACAG	CGTAGACTAG	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15J	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	TACGAGTATG	TACTCTCGTG	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15K	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	TCGTCGCTCG		LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15L	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	ACGCGAGTAT		LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15M	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15N	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	AGACTATACT	AGCGTCGTCT	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15O	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	AGTACGCTAT	ATAGAGTACT	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15Q	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	CGACGTGACT	TACACACACT	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15R	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	TACACGTGAT		LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15S	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	TACGCTGTCT	TAGTGTAGAT	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15T	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	TCGATCACGT	TCGCACTAGT	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15V	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	TGACGTATGT		LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15W	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	ACGCGATCGA		LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15X	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	ACTAGCAGTA	AGCTCACGTA	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-15Y	LA-ST_BM_Pool_07_S12_L001_R1_001.fastq.gz	LA-ST_BM_Pool_07_S12_L001_R2_001.fastq.gz	AGTATACATA	AGTCGAGAGA	LA-15	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-24D	LA-ST_BM_Pool_08_S13_L001_R1_001.fastq.gz	LA-ST_BM_Pool_08_S13_L001_R2_001.fastq.gz	ACGAGTGCGT	ACGCTCGACA	LA-24	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-24E	LA-ST_BM_Pool_08_S13_L001_R1_001.fastq.gz	LA-ST_BM_Pool_08_S13_L001_R2_001.fastq.gz	AGACGCACTC	AGCACTGTAG	LA-24	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-24F	LA-ST_BM_Pool_08_S13_L001_R1_001.fastq.gz	LA-ST_BM_Pool_08_S13_L001_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	LA-24	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-24G	LA-ST_BM_Pool_08_S13_L001_R1_001.fastq.gz	LA-ST_BM_Pool_08_S13_L001_R2_001.fastq.gz	CTCGCGTGTC	TCTCTATGCG	LA-24	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-24I	LA-ST_BM_Pool_08_S13_L001_R1_001.fastq.gz	LA-ST_BM_Pool_08_S13_L001_R2_001.fastq.gz	CGAGAGATAC	ATACGACGTA	LA-24	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-24J	LA-ST_BM_Pool_08_S13_L001_R1_001.fastq.gz	LA-ST_BM_Pool_08_S13_L001_R2_001.fastq.gz	TCACGTACTA		LA-24	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-24L	LA-ST_BM_Pool_08_S13_L001_R1_001.fastq.gz	LA-ST_BM_Pool_08_S13_L001_R2_001.fastq.gz	ACGACTACAG	CGTAGACTAG	LA-24	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-24N	LA-ST_BM_Pool_08_S13_L001_R1_001.fastq.gz	LA-ST_BM_Pool_08_S13_L001_R2_001.fastq.gz	TCGTCGCTCG		LA-24	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-24Q	LA-ST_BM_Pool_08_S13_L001_R1_001.fastq.gz	LA-ST_BM_Pool_08_S13_L001_R2_001.fastq.gz	AGACTATACT	AGCGTCGTCT	LA-24	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-24R	LA-ST_BM_Pool_08_S13_L001_R1_001.fastq.gz	LA-ST_BM_Pool_08_S13_L001_R2_001.fastq.gz	AGTACGCTAT	ATAGAGTACT	LA-24	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-24T	LA-ST_BM_Pool_08_S13_L001_R1_001.fastq.gz	LA-ST_BM_Pool_08_S13_L001_R2_001.fastq.gz	CGACGTGACT		LA-24	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-25A	LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz	LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz	ACGAGTGCGT	ACGCTCGACA	LA-25	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-25B	LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz	LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz	AGACGCACTC	AGCACTGTAG	LA-25	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-25D	LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz	LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	LA-25	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-25F	LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz	LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz	TGATACGTCT		LA-25	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-25G	LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz	LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz	CGAGAGATAC	ATACGACGTA	LA-25	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-25CS	LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz	LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz	TCACGTACTA		LA-25	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-25CT	LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz	LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz	TGTACTACTC		LA-25	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-25CU	LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz	LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz	CGTAGACTAG		LA-25	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-25CV	LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz	LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz	TACGAGTATG	TACTCTCGTG	LA-25	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-25CY	LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz	LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	LA-25	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-25DA	LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz	LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz	AGTACGCTAT		LA-25	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-25DC	LA-ST_BM_Pool_09_S14_L001_R1_001.fastq.gz	LA-ST_BM_Pool_09_S14_L001_R2_001.fastq.gz	CGACGTGACT	TACACACACT	LA-25	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26B	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	AGACGCACTC		LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26C	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26D	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	CTCGCGTGTC	TCTCTATGCG	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26E	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	TGATACGTCT		LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26F	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	CGAGAGATAC	ATACGACGTA	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26G	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	TCACGTACTA		LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26H	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	TGTACTACTC		LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26I	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	ACGACTACAG	CGTAGACTAG	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26J	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	TACGAGTATG	TACTCTCGTG	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26K	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	TCGTCGCTCG		LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26L	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	ACATACGCGT	ACGCGAGTAT	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26M	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26N	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	AGACTATACT	AGCGTCGTCT	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26O	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	AGTACGCTAT		LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26P	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	CACGCTACGT	CAGTAGACGT	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26R	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	CGACGTGACT	TACACACACT	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26T	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	TACGCTGTCT	TAGTGTAGAT	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26U	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	TCGATCACGT	TCGCACTAGT	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26V	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	TCTAGCGACT	TCTATACTAT	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
LA-26W	LA-ST_BM_Pool_10_S15_L001_R1_001.fastq.gz	LA-ST_BM_Pool_10_S15_L001_R2_001.fastq.gz	TGACGTATGT	TGTGAGTAGT	LA-26	Laguna Atascosa National Wildlife Refuge, Texas, USA
ST-07C	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	CTCGCGTGTC	TCTCTATGCG	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-14A	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	CATAGTAGTG		Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-17D	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	TCACGTACTA		Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-20A	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	TCTACGTAGC	TGTACTACTC	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-24B	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	CGTAGACTAG		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-25C	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	TACGAGTATG		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-40B	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	TAGAGACGAG	TCGTCGCTCG	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-44C	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	ACATACGCGT	ACGCGAGTAT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-45C	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-47E	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	AGACTATACT	AGCGTCGTCT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-55C	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	AGTACGCTAT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-56E	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	CACGCTACGT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-58E	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	CGACGTGACT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-60D	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	TACAGATCGT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-62C	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	TACGCTGTCT	TAGTGTAGAT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-65C	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	TCGATCACGT	TCGCACTAGT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-72A	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	TCTAGCGACT		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-76H	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	TGACGTATGT		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-78I	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	ACAGTATATA		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-80H	GCSNAIndex-1_S1_L001_R1_001.fastq.gz GCSNAIndex-1_S1_L002_R1_001.fastq.gz GCSNAIndex-1_S1_L003_R1_001.fastq.gz GCSNAIndex-1_S1_L004_R1_001.fastq.gz	GCSNAIndex-1_S1_L001_R2_001.fastq.gz GCSNAIndex-1_S1_L002_R2_001.fastq.gz GCSNAIndex-1_S1_L003_R2_001.fastq.gz GCSNAIndex-1_S1_L004_R2_001.fastq.gz	ACGCGATCGA		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-01B	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	ACGCTCGACA		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-02D	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	AGCACTGTAG		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-04B	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-08A	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	CTCGCGTGTC	ACTAGCAGTA	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-14B	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	TGATACGTCT	CATAGTAGTG	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-18A	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	TCACGTACTA	CGTCTAGTAC	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-45D	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-48D	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	AGACTATACT	AGCGTCGTCT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-55D	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	ATAGAGTACT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-57B	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	CACGCTACGT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-59A	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	CGACGTGACT	TACACACACT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-60E	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	TACAGATCGT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-62E	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	TACGCTGTCT	TAGTGTAGAT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-65D	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	TCGATCACGT	TCGCACTAGT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-72H	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	TCTAGCGACT	TCTATACTAT	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-77E	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	TGACGTATGT		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-80B	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	ACGCGATCGA		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-81B	GCSNAIndex-2_S2_L001_R1_001.fastq.gz GCSNAIndex-2_S2_L002_R1_001.fastq.gz GCSNAIndex-2_S2_L003_R1_001.fastq.gz GCSNAIndex-2_S2_L004_R1_001.fastq.gz	GCSNAIndex-2_S2_L001_R2_001.fastq.gz GCSNAIndex-2_S2_L002_R2_001.fastq.gz GCSNAIndex-2_S2_L003_R2_001.fastq.gz GCSNAIndex-2_S2_L004_R2_001.fastq.gz	ACTAGCAGTA		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-01C	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	ACGAGTGCGT	ACGCTCGACA	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-02E	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	AGCACTGTAG		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-04C	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-08C	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	CTCGCGTGTC		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-14C	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	CATAGTAGTG		Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-18B	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	TCACGTACTA		Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-44E	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	ACATACGCGT	ACGCGAGTAT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-45E	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-51A	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	AGACTATACT	AGCGTCGTCT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-55E	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	AGTACGCTAT	ATAGAGTACT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-57C	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	CACGCTACGT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-59B	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	CGACGTGACT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-60F	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	TACAGATCGT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-63C	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	TACGCTGTCT	TAGTGTAGAT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-65E	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	TCGATCACGT	TCGCACTAGT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-74E	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	TCTATACTAT		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-78C	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	TGACGTATGT		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-80F	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	ACAGTATATA		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-81I	GCSNAIndex-3_S3_L001_R1_001.fastq.gz GCSNAIndex-3_S3_L002_R1_001.fastq.gz GCSNAIndex-3_S3_L003_R1_001.fastq.gz GCSNAIndex-3_S3_L004_R1_001.fastq.gz	GCSNAIndex-3_S3_L001_R2_001.fastq.gz GCSNAIndex-3_S3_L002_R2_001.fastq.gz GCSNAIndex-3_S3_L003_R2_001.fastq.gz GCSNAIndex-3_S3_L004_R2_001.fastq.gz	ACTAGCAGTA		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-03B	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	AGCACTGTAG		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-05C	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-08E	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	CTCGCGTGTC	TCTCTATGCG	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-14D	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	CATAGTAGTG		Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-18E	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	TCACGTACTA	CGTCTAGTAC	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-22B	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	TCTACGTAGC		Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-25A	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	ACGACTACAG	CGTAGACTAG	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-41E	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	TCGTCGCTCG		Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-45A	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	ACATACGCGT	ACGCGAGTAT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-46B	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-51D	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	AGACTATACT	AGCGTCGTCT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-56A	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	AGTACGCTAT	ATAGAGTACT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-57E	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	CACGCTACGT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-59C	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	CGACGTGACT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-61C	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	TACAGATCGT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-64A	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	TACGCTGTCT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-67E	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	TCGATCACGT	TCGCACTAGT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-75E	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	TCTAGCGACT		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-81J	GCSNAIndex-4_S4_L001_R1_001.fastq.gz GCSNAIndex-4_S4_L002_R1_001.fastq.gz GCSNAIndex-4_S4_L003_R1_001.fastq.gz GCSNAIndex-4_S4_L004_R1_001.fastq.gz	GCSNAIndex-4_S4_L001_R2_001.fastq.gz GCSNAIndex-4_S4_L002_R2_001.fastq.gz GCSNAIndex-4_S4_L003_R2_001.fastq.gz GCSNAIndex-4_S4_L004_R2_001.fastq.gz	ACTAGCAGTA		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-01E	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	ACGAGTGCGT	ACGCTCGACA	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-05E	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	ATATCGCGAG	CGTGTCTCTA	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-11A	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	CTCGCGTGTC	TCTCTATGCG	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-14E	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	CATAGTAGTG		Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-19B	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	TCACGTACTA		Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-25B	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	ACGACTACAG		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-43B	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	TAGAGACGAG	TCGTCGCTCG	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-46C	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-53A	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	AGACTATACT		Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-56B	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	AGTACGCTAT	ATAGAGTACT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-58C	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	CACGCTACGT	CAGTAGACGT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-59D	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	CGACGTGACT	TACACACACT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-61E	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	TACAGATCGT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-64C	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	TACGCTGTCT	TAGTGTAGAT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-68A	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	TCGATCACGT	TCGCACTAGT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-75I	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	TCTAGCGACT	TCTATACTAT	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-82G	GCSNAIndex-5_S5_L001_R1_001.fastq.gz GCSNAIndex-5_S5_L002_R1_001.fastq.gz GCSNAIndex-5_S5_L003_R1_001.fastq.gz GCSNAIndex-5_S5_L004_R1_001.fastq.gz	GCSNAIndex-5_S5_L001_R2_001.fastq.gz GCSNAIndex-5_S5_L002_R2_001.fastq.gz GCSNAIndex-5_S5_L003_R2_001.fastq.gz GCSNAIndex-5_S5_L004_R2_001.fastq.gz	ACTAGCAGTA		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-02A	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	ACGAGTGCGT	ACGCTCGACA	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-11B	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	CTCGCGTGTC	TCTCTATGCG	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-15A	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	TGATACGTCT	CATAGTAGTG	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-19C	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	TCACGTACTA	CGTCTAGTAC	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-21E	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	TCTACGTAGC	TGTACTACTC	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-24D	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	ACGACTACAG	CGTAGACTAG	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-40D	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	TCGTCGCTCG		Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-47A	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-55B	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	AGACTATACT	AGCGTCGTCT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-56C	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	AGTACGCTAT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-58D	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	CACGCTACGT	CAGTAGACGT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-60A	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	CGACGTGACT	TACACACACT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-62B	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	TACACGTGAT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-65A	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	TACGCTGTCT	TAGTGTAGAT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-69D	GCSNAIndex-6_S6_L001_R1_001.fastq.gz GCSNAIndex-6_S6_L002_R1_001.fastq.gz GCSNAIndex-6_S6_L003_R1_001.fastq.gz GCSNAIndex-6_S6_L004_R1_001.fastq.gz	GCSNAIndex-6_S6_L001_R2_001.fastq.gz GCSNAIndex-6_S6_L002_R2_001.fastq.gz GCSNAIndex-6_S6_L003_R2_001.fastq.gz GCSNAIndex-6_S6_L004_R2_001.fastq.gz	TCGATCACGT	TCGCACTAGT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-82J	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	ACGAGTGCGT	ACGCTCGACA	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-02C	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	AGCACTGTAG		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-03D	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	CGTGTCTCTA		Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-11E	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	CTCGCGTGTC	TCTCTATGCG	Mad Crow Cave	Government Canyon State Natural Area, Texas, USA
ST-15B	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	TGATACGTCT	CATAGTAGTG	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-19E	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	TCACGTACTA	CGTCTAGTAC	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-20B	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	TCTACGTAGC	TGTACTACTC	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-24C	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	CGTAGACTAG		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-40C	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	TAGAGACGAG	TCGTCGCTCG	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-44D	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	ACATACGCGT	ACGCGAGTAT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-47D	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	ACTACTATGT	ACTGTACAGT	Bone Pile Cave	Government Canyon State Natural Area, Texas, USA
ST-56D	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	AGTACGCTAT	ATAGAGTACT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-60B	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	CGACGTGACT		Little Crevice Cave	Government Canyon State Natural Area, Texas, USA
ST-65B	GCSNAIndex-7_S7_L001_R1_001.fastq.gz GCSNAIndex-7_S7_L002_R1_001.fastq.gz GCSNAIndex-7_S7_L003_R1_001.fastq.gz GCSNAIndex-7_S7_L004_R1_001.fastq.gz	GCSNAIndex-7_S7_L001_R2_001.fastq.gz GCSNAIndex-7_S7_L002_R2_001.fastq.gz GCSNAIndex-7_S7_L003_R2_001.fastq.gz GCSNAIndex-7_S7_L004_R2_001.fastq.gz	TACGCTGTCT	TAGTGTAGAT	Little Crevice Cave	Government Canyon State Natural Area, Texas, USA

Code/Software

Data was analysed as follows

Sequences from R1 and R2 files were merged using BBMerge https://jgi.doe.gov/data-and-tools/software-tools/bbtools/

sh bbmerge.sh in1=InputFilename.fastq in2=InputFilename.fastq.gz out=OutputFilename.fastq

Merged sequencing reads were analysed using SeekDeep. Hathaway, N. J., Parobek, C. M., Juliano, J. J., & Bailey, J. A. (2018). SeekDeep: Single-base resolution de novo clustering for amplicon deep sequencing. Nucleic Acids Research, 46(4), e21–e21. https://doi.org/10.1093/nar/gkx1201

See https://seekdeep.brown.edu/ for downloads and tutorial

SeekDeep Extractor

SeekDeep extractor --fastq Filename.fastq --minLen 100 --qualCheckLevel 25 --qualCheckCutOff .75 --midWithinStart 20 --id id.txt --illumina --verbose --checkRevComplementForMids --backEndPrimerWithinStart 50

SeekDeep Qluster

SeekDeep qluster --fastq ExtractorOutputFile.fastq --illumina --verbose --writeoutinitalseqs

SeekDeep Process Clusters

SeekDeep processClusters --fastq Qlusteroutput.fastq --noErrors --illumina

Output sequences were then matched with NCBI BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) Nucelotide

The script.R file relates to the statistcal analysis presesented in the manuscript.