Ancient plant DNA reveals High Arctic greening during the Last Interglacial
Crump, Sarah (2021), Ancient plant DNA reveals High Arctic greening during the Last Interglacial, Dryad, Dataset, https://doi.org/10.5061/dryad.pk0p2ngmq
We extracted and analyzed sedimentary ancient DNA (sedaDNA) from a lake sediment core from Baffin Island, Arctic Canada, that spans the Holocene and Last Interglacial. We primarily used a metabarcoding approach, targeting the P6 loop of the chloroplast trnL (UAA) intron to identify vascular plant DNA through time. For select samples, we also prepared and sequenced single-stranded shotgun libraries. See "Materials and Methods" section of the publication for methodological details.
The metabarcoding summary table, "CrumpEtAl2021_MetabarcodingSummaryTable.csv", includes post-filtered taxonomic results based on the metabarcoding methods outlined in the publication. The zipped .fastq files are demultiplexed (one file per sample) sequences with primers and adapters removed, but no further processing steps performed. See "README_CrumpEtAl2021_DNAmetabarcodingDataset.txt" and "README_CrumpEtAl2021_ShotgunDataset.txt" for additional usage notes.
National Science Foundation, Award: 1737712
National Science Foundation, Award: 1737716
National Science Foundation, Award: 1737750
National Science Foundation, Award: 1144083
National Science Foundation, Award: 1657743