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Data from: Trade-offs between morphology and thermal niches mediate adaptation in response to competing selective pressures

Citation

Uiterwaal, Stella et al. (2020), Data from: Trade-offs between morphology and thermal niches mediate adaptation in response to competing selective pressures, Dryad, Dataset, https://doi.org/10.5061/dryad.pzgmsbcgb

Abstract

The effects of climate change - such as increased temperature variability and novel predators – rarely happen in isolation, but it is unclear how organisms cope with multiple stressors simultaneously. To explore this, we grew replicate Paramecium caudatum populations in either constant or variable temperatures and exposed half to predation. We then fit thermal performance curves (TPCs) of intrinsic growth rate (rmax) for each replicate population (N = 12) across seven temperatures (10°C - 38°C). TPCs of P. caudatum exposed to both temperature variability and predation responded only to one or the other (but not both), resulting in unpredictable outcomes. These changes in TPCs were accompanied by changes in cell morphology. Although cell volume was conserved across treatments, cells became narrower in response to temperature variability and rounder in response to predation. Our findings suggest that predation and temperature variability produce conflicting pressures on both thermal performance and cell morphology. Lastly, we found a strong correlation between changes in cell morphology and TPC parameters in response to predation, suggesting that responses to opposing selective pressures could be constrained by trade-offs. Our results shed new light on how environmental and ecological pressures interact to elicit changes in characteristics at both the individual and population levels. We further suggest that morphological responses to interactive environmental forces may modulate population-level responses, making prediction of long-term responses to environmental change challenging.

Methods

Please see the associated manuscript, "Trade-offs between morphology and thermal niches mediate adaptation in response to competing selective pressures", in Ecology and Evolution for data collection methods.

Usage Notes

CellSizeMeasurements - FlowCam data for cells from each population. "Clim" describes temperature variability of the cell's populations (0 for 29±0°C and 4 for 29±4°C). "Pred?" describes whether the population was exposed to predation ("Y") or not ("N"). Population refers to the initial starting population. Units are μm or μm2. ESD = Estimated Spherical Diameter. Please note that volume here is calculated by the FlowCam and assumes a spherical shape. 

TPCdata - Data from the thermal performance curve experiment. "Pop" refers to the intial starting population. "Clim" describes temperature variability of the cell's populations (0 for 29±0°C and 4 for 29±4°C). "Pred" describes whether the population was exposed to predation ("Y") or not ("N"). "Temp" is the temperature of the incubator. "Rep" is the Petri dish replicate number. "Count" is the final number of cells in each Petri dish. "Time in" and "Time out" are when the Petri dish went in and out of the incubator, respectively. "Initials" are the initials of the person who recorded the "Count" data for that Petri dish. 

Funding

National Science Foundation, Award: DGE-1610400

National Science Foundation, Award: IOS-1838098