Regional and fine-scale local adaptation in salinity tolerance in Daphnia inhabiting contrasting clusters of inland saline waters
Data files
Jan 17, 2024 version files 107.81 KB
Abstract
Freshwater salinisation is an important threat to biodiversity, ecosystem functioning, and the provision of ecosystem services. Therefore, understanding the capacity of species to adapt to salinity gradients is crucial. Clusters of naturally saline habitats represent ideal test cases to study the extent and scale of local adaptation to salinisation. We studied local genetic adaptation of the water flea Daphnia magna, a key component of pond food webs, to salinity in two contrasting landscapes - a dense cluster of sodic bomb crater ponds and a larger-scale cluster of soda pans. We show regional differentiation in salinity tolerance reflecting the higher salinity levels of soda pans versus bomb crater ponds. We found local adaptation to differences in salinity levels at the scale of tens of metres among bomb crater pond populations but not among geographically more distant soda pan populations. The population-level salinity tolerance range was reduced in more saline bomb crater ponds through an upward shift of the minimum salt tolerance observed across clones and a consequent gradual loss of less tolerant clones in a nested pattern. Our results show genetic adaptation to salinity gradients at different spatial scales and fine-tuned local adaptation in neighbouring habitat patches in a natural landscape.
README: Data from: Regional and fine-scale local adaptation in salinity tolerance in Daphnia inhabiting contrasting clusters of inland saline waters
https://doi.org/10.5061/dryad.pzgmsbctw
Brief summary
The data package contains two datasets:
- Brans_et_al_data_2024_raw.csv
- Brans_et_al_data_2024_EC50 and environmental variables.csv
Datasets of the recorded variables
(1) Brans_et_al_data_2024_raw.csv
This dataset contains information on the survival of Daphnia magna after 24 and 48 hours of exposure to the experimental salinity levels. For details on the experiment, sampling, and sample processing, see Brans et al. (2024).
Variable names with descriptions:
*pond type: type of original habitat of the Daphnia populations, i.e. soda pan (SP) or bomb crater pond (BC).
*pop: The ID of the pond of origin of each Daphnia population. IDs of bomb crater ponds are numbers given by the authors at the first mapping of all ponds in 2014. Abbreviations for soda pans: APME - Apetloner Meierhoflacke, HERR - Herrnsee, MSTI - Mittlerer Stinkersee, OWOR - Östliche Wörthenlacke, RUND - Runde Lacke, SECH - Sechsmadlacke, ZICK - Zicklacke. For the geographic coordinates of the habitats, see Brans et al. (2024).
*clone: The ID of each clone within the populations.
*clone_id: Unique ID of each clone.
*conc: Experimental salt (NaHCO3) concentration (g/l).
*replicate: Number of replicate on the clonal level out of 3 replicates.
*mortality_24: Number of dead individuals after 24 hours of exposure.
*mortality_48: Number of dead individuals after 48 hours of exposure.
*total amount animals: Number of Daphnia individuals used per experimental replicate.
(2) Brans_et_al_data_2024_EC50 and environmental variables.csv
This dataset contains the calculated EC50 values as well as the local environmental characteristics of the ponds.
Variable names with descriptions:
*clone_id: As in Brans_et_al_data_2024_raw.csv.
*EC50_24h: EC50 values calculated from mortality after 24 hours based on 3 replicates at the clonal level as described in Brans et al. (2024).
*EC50_48h: EC50 values calculated from mortality after 48 hours based on 3 replicates at the clonal level as described in Brans et al. (2024).
*pond type: As in Brans_et_al_data_2024_raw.csv.
*pop: As in Brans_et_al_data_2024_raw.csv.
*cond: Conductivity (mS/cm) measured at the field as described in Brans et al. (2024).
*sal: Pond salinity (g/L) calculated from conductivity as described in Brans et al. (2024).
*TSS: Concentration of total suspended solids (g/L) measured at the field as described in Brans et al. (2024).
*chla: Concentration of chlorophyll a (ug/L) measured at the field as described in Brans et al. (2024).
*pH: pH measured at the field as described in Brans et al. (2024).
*water.depth: Water depth (cm) of the ponds measured at the field as described in Brans et al. (2024).
*mean_spring: Mean spring salinity (g/L) calculated as described in Brans et al. (2024).
*mean_summer: Mean summer salinity (g/L) calculated as described in Brans et al. (2024).