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Data from: Polybrominated diphenyl ether (DE-71) exposure skews phenotypic sex ratio, and alters steroid hormone levels and steroidogenic enzyme activities in juvenile Silurana tropicalis

Citation

Fort, Douglas J. et al. (2019), Data from: Polybrominated diphenyl ether (DE-71) exposure skews phenotypic sex ratio, and alters steroid hormone levels and steroidogenic enzyme activities in juvenile Silurana tropicalis, Dryad, Dataset, https://doi.org/10.5061/dryad.q4qd540

Abstract

The impact of the brominated flame-retardant mixture, DE-71, on gonadal steroidogenesis during sexual differentiation in Silurana tropicalis was examined. A partial lifecycle study exposing S. tropicalis to varying concentrations of DE-71 (0.0, 0.65, 1.3, 2.5, and 5.0 g/L [nominal]) was conducted from early gastrula stage embryo to 150 d post-metamorphosis (dpm). Exposure of S. tropicalis to DE-71 induced liver necrosis and induced abnormal ovary development characterized by previtellogenic oocyte necrosis and arrested development of vitellogenic oocytes in females in a concentration-dependent manner. Decreased mean plasma DHT and T, gonad T, and increased mean plasma E2 levels were found in 150 dpm DE-71-treated male S. tropicalis compared to controls. Plasma E2 levels in females were not significantly altered compared to control S. tropicalis, although lower plasma and gonad T were detected. Mean gonadal CYP 19 aromatase activity in both male and female S. tropicalis exposed to DE-71 was not appreciably affected. Decreased mean male 5α-reductase and CYP17 activities in both male and females were observed compared to control frogs. Overall, these studies suggested that PBDE exposure induced liver necrosis and abnormal ovary development; and reduced circulating and gonadal androgens resulting in a phenotypic skew in sex ratio toward the female sex in S. tropicalis

Usage Notes

Funding

National Science Foundation, Award: No

Location

USA