Data from: A study of the transit amplification divisions during spermatogenesis in Oncopeltus fasciatus to assess plasticity in sperm numbers or sperm viability under different diets
Duxbury, Ashley E., University of Georgia
Weathersby, Brandie, University of Georgia
Sanchez, Zachary, University of Georgia
Moore, Patricia J., University of Georgia
Published May 07, 2019 on Dryad.
Cite this dataset
Duxbury, Ashley E.; Weathersby, Brandie; Sanchez, Zachary; Moore, Patricia J. (2019). Data from: A study of the transit amplification divisions during spermatogenesis in Oncopeltus fasciatus to assess plasticity in sperm numbers or sperm viability under different diets [Dataset]. Dryad. https://doi.org/10.5061/dryad.q57n9h7
Oncopeltus fasciatus males fed the ancestral diet of milkweed seeds prioritize reproduction over lifespan as evidenced by higher rates of fertility and shorter lifespans than males from the same population fed the adapted diet of sunflower seeds. We examined the proximate mechanisms by which milkweed-fed males maintained late-life fertility. We tested the hypothesis that older milkweed-fed males maintained fertility by producing more, higher quality sperm. Our results, that older males have more sperm, but their sperm do not have higher viability, is in general agreement with other recent studies on how nutrition affects male fertility in insects. We further examined the mechanisms by which sperm are produced by examining the progression of spermatogonial cells through the cell cycle during the transit amplification divisions. We demonstrated that diet affects the likelihood of a spermatocyst being in the S-phase or M-phase of the cell cycle. Given work in model systems, these results have implications for subtle effects on sperm quality either through replication stress or epigenetic markers. Thus, viability may not be the best marker for sperm quality and more work is called for on the mechanisms by which the germline and the production of sperm mediate the cost of reproduction.
Fecundity and Fertility data for Figure 4
Excel spreadsheet containing data collected on laboratory populations of Oncopeltus fasciatus. Data was collected for females mated to males at 2-weeks or 4-weeks post adult emergence that had been reared on either a diet of sunflower or milkweed seeds following adult emergence. The total number of eggs laid was counted. The total number of eggs that showed evidence of fertilization was also counted and the percent of eggs fertilized was calculated.
fecundity and fertility data.xls
Sperm Count data for figure 5
Excel spreadsheet with data on sperm counts of males fed a diet of either milkweed or sunflower seeds from adult emergence. Sperm counts were calculated from a subsample. Sperm counts estimate the total number of sperm stored in the seminal vesicle at the time of dissection.
spermatocyst cell division rates
Excel spreadsheet with data on the number of spermatocysts staining positive for EdU incorporation or phospho-histone H3 modification. Data taken from the testes of males fed a diet of milkweed or sunflower seeds after adult emergence.