All diseases are characterized by distinct changes in tissue molecular distribution. Molecular analysis of intact tissues traditionally requires pre-existing knowledge of, and reagents for, the targets of interest. Conversely, label-free discovery of disease-associated tissue analytes requires destructive processing for downstream identification platforms. Tissue-based analyses therefore sacrifice discovery to gain spatial distribution of known targets, or sacrifice tissue architecture for discovery of unknown targets. To overcome these obstacles, we developed a multi-modality imaging platform for discovery-based molecular histology. We apply this platform to a model of disseminated infection triggered by the important pathogen Staphylococcus aureus, leading to the discovery of infection-associated alterations in the distribution and abundance of proteins and elements in tissue. These data provide an unbiased, three-dimensional analysis of how disease impacts the molecular architecture of complex tissues, enable culture-free diagnosis of infection through imaging-based detection of bacterial and host analytes, and reveal molecular heterogeneity at the host-pathogen interface.
ICP 3D Volume
These data are LA-ICP IMS data from a mouse kidney infected with S. aureus. Each section of the mouse is numbered and data within each folder correspond to each section.
ICP Iron Reporter
These data are LA-ICP IMS data corresponding to mouse kidneys infected with S.aureus. These mice were infected with a, iron-reporter. Mice were analyzed at three time points post infection: 4, 7, and 10 days
ICP Mn Reporter
These are LA-ICP IMS data from the kidney of mice infected with S. aureus. This stain of S. aureus is responsive to manganese. Mice were analyzed at 4, 7, and 10 days post infection.
bottom up
This file represents LC-MS/MS targeted analysis of a peptide detected in MALDI IMS data, delta hemolysin. The sample is a fraction purified from mouse kidney infected with S. aureus. The samples was tryptically digested prior to analysis with LC-MS/MS
Bottom up.zip
FTICR
MALDI FTICR IMS of a mouse section containing an infectious foci. The FTICR was used to obtain accurate mass measurements from the MALDI IMS data in order to have confidence in protein identifications.
MALDI 3D Volume
These data are MALDI IMS data from a 3D mouse kidney infected with S. aureus. These were collected using a SimulTOF mass spectrometer.
Top Down
These data represent LC-MS/MS analysis of intact proteins directly extracted from infectious foci of mouse kidneys infected with S. aureus. Proteins were fragmented using ETD, and ions matching ions detected in MALDI IMS data were manually sequenced.
MALDI Iron 4 Day
These data represent MALDI IMS data of biological replicates of mice infected with S. aureus 4 days post infection. This particular strain of S. aureus has been modified with a fluorescent reporter sensitive to iron deplete conditions. Three biological replicates are tested here.
MALDI Fe Day 4.zip
MALDI Iron 7 Day
These data represent MALDI IMS data of biological replicates of mice infected with S. aureus 7 days post infection. This particular strain of S. aureus has been modified with a fluorescent reporter sensitive to iron deplete conditions. Three biological replicates are tested here.
MALDI Fe Day 7.zip
MALDI Iron 10 Day
These data represent MALDI IMS data of biological replicates of mice infected with S. aureus 10 days post infection. This particular strain of S. aureus has been modified with a fluorescent reporter sensitive to iron deplete conditions. Three biological replicates are tested here.
MALDI Fe_10DPI.zip
MALDI manganese 4 Day
These data represent MALDI IMS data of biological replicates of mice infected with S. aureus 4 days post infection. This particular strain of S. aureus has been modified with a fluorescent reporter sensitive to manganese deplete conditions. Three biological replicates are tested here.
MALDI Mn_day4.zip
MALDI manganese 7 Day
These data represent MALDI IMS data of biological replicates of mice infected with S. aureus 7 days post infection. This particular strain of S. aureus has been modified with a fluorescent reporter sensitive to manganese deplete conditions. Three biological replicates are tested here.
MALDI Mn_day7.zip
MALDI manganese 10 Day
These data represent MALDI IMS data of biological replicates of mice infected with S. aureus 4 days post infection. This particular strain of S. aureus has been modified with a fluorescent reporter sensitive to manganese deplete conditions. Three biological replicates are tested here.
MALDI Mn_10DPI.zip